Tri 3d bone software

To analyze the structural alteration of the knee joint after treadmill running and/or OVX, right femurs were harvested and fixed in 70 % ethanol. Skeletal imaging was captured using a micro-CT apparatus (Scan Xmate-E090; Comscan Techno Co., Kanagawa, Japan). Both metaphyseal and epiphyseal bone volume per tissue volume (BV/TV), trabecular spacing (Tb. Spac.), trabecular thickness (Tb. Th.), trabecular number (Tb. N.), and subchondral bone plate thickness (Sb. Pl. Th.) of the tibia were calculated using Tri/3D-BONE software (Ratoc System Engineering Co., Tokyo, Japan). The trabecular bone parameter was measured in the metaphyseal region 300–800 μm proximal from the edge of the growth plate.

The calvarial bones were observed under anesthesia using micro-computed tomography (micro-CT) (COSMO Scan GX, Rigaku, Tokyo, Japan) 4 weeks after the surgical procedure [23 (link)]. Micro-CT was performed after 18 s of exposure. The tube voltage was 90 kV, the tube current was 88 μA, and the image size was 512 × 512 pixels. Three-dimensional images, which contained horizontal sections and sagittal sections, were reconstructed and volumes of newly formed bone areas were calculated using TRI/3DBONE software (RATOC System Engineering, Tokyo, Japan).