Kod polymerase master mix

Manufactured by Merck Group

KOD polymerase master mix is a ready-to-use solution containing a high-fidelity DNA polymerase, buffer, dNTPs, and additional components optimized for efficient and accurate DNA amplification.

Automatically generated - may contain errors

Lab products found in correlation

Anti cd28 cd28.2 activating antibodies, by Thermo Fisher Scientific (2 mentions) Anti cd3 okt3, by Thermo Fisher Scientific (2 mentions) Human ab serum, by Omega Scientific (2 mentions) Polybrene, by Merck Group (2 mentions) Cell culture media, by Corning (2 mentions) Fetal bovine serum (fbs), by Corning (2 mentions) Fluorescently labeled antibodies, by BioLegend (1 mentions) Retronectin, by Takara Bio (1 mentions) Concanavalin a (cona), by Merck Group (1 mentions) Pha l, by Merck Group (1 mentions) Phytohemagglutinin l, by Merck Group (1 mentions) Poly l lysine (pll), by Merck Group (1 mentions) 7 aad, by BD (1 mentions)

2 protocols using kod polymerase master mix

T Cell Activation and Expansion Protocol

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Primers were purchased from Integrated DNA Technologies (Coralville, IA). KOD polymerase master mix and polybrene were purchased from EMD Millipore (Darmstadt, Germany). Sequencing was performed by Retrogen Inc (San Diego, CA). Fluorescently labeled antibodies and 7-AAD for flow cytometry were purchased from Biolegend (San Diego, CA), eBioscience (San Diego, CA), or Beckman Coulter (Brea, CA). Peptides were purchased from Anaspec Inc (Fremont, CA) or Thermo Fisher Scientific (Waltham, MA). Anti-CD3 (OKT3) and anti-CD28 (CD28.2) activating antibodies were purchased from eBioscience (San Diego, CA). Short-dated Proleukin was provided by Prometheus Laboratories Inc (San Diego, CA) through an investigator-initiated trial program. All other cytokines were purchased from Peprotech, Inc. (Rocky Hill, NJ). Concanavalin A was purchased from Sigma-Aldrich (St. Louis, MO). RetroNectin was purchased from Clontech Laboratories (Mountain View, CA). BioT transfection reagent was purchased from Bioland Scientific (Paramount, CA). Cell culture media, antibiotics, and fetal bovine serum were purchased from Corning (Corning, NY). Human AB serum was purchased from Omega Scientific (Tarzana, CA). Peptides were purchased from Anaspec (San Jose, CA).

Bethune M.T., Gee M.H., Bunse M., Lee M.S., Gschweng E.H., Pagadala M.S., Zhou J., Cheng D., Heath J.R., Kohn D.B., Kuhns M.S., Uckert W, & Baltimore D. (2016). Domain-swapped T cell receptors improve the safety of TCR gene therapy. eLife, 5, e19095.

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Detailed Reagents and Materials Procurement

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Peptides were purchased from Anaspec, Thermo Fisher Scientific, and Mimotopes. Fluorescent antibodies and 7-AAD used for flow cytometry were purchased from BD Biosciences, BioLegend, or eBioscience. Fluorescent peptide–MHC multimers were purchased from TCMetrix or prepared in-house as described (50 (link)) from biotinylated monomers [obtained from NIH Tetramer Core, or expressed heterologously in Escherichia coli, refolded, and biotinylated in-house as described (51 (link))]. Primers were purchased from Integrated DNA Technologies. KOD polymerase master mix and polybrene were purchased from EMD Millipore. Sequencing was performed by Retrogen Inc. Anti-CD3 (OKT3) and anti-CD28 (CD28.2) activating antibodies were purchased from eBioscience. Cytokines were purchased from Peprotech, Inc. BioT transfection reagent was purchased from Bioland Scientific. Cell culture media, antibiotics, and FBS were purchased from Corning. Human AB serum was purchased from Omega Scientific. Poly-l-lysine and PHA-L (phytohemagglutinin-L) were purchased from Sigma.

Bethune M.T., Li X.H., Yu J., McLaughlin J., Cheng D., Mathis C., Moreno B.H., Woods K., Knights A.J., Garcia-Diaz A., Wong S., Hu-Lieskovan S., Puig-Saus C., Cebon J., Ribas A., Yang L., Witte O.N, & Baltimore D. (2018). Isolation and characterization of NY-ESO-1–specific T cell receptors restricted on various MHC molecules. Proceedings of the National Academy of Sciences of the United States of America, 115(45), E10702-E10711.

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