For assays investigating apoptosis inhibition, cells were incubated with 20 μM Z-VAD-fmk for 4 hr prior to the addition of SPs. After being treated with L-, D-, and DL-SPs (1.7 × 108 particles/mL) for 48 hr, cell viability assay was measured using the ATP CellTiter-Glo® luminescent cell viability assay.
Celltiter glo luminescent cell viability assay atp
The CellTiter-Glo® Luminescent Cell Viability Assay is a quantitative method for determining the number of viable cells in a cell-based assay. The assay measures the amount of ATP present, which is an indicator of the metabolically active cells. The assay uses a proprietary thermostable luciferase to catalyze the conversion of luciferin and ATP to oxyluciferin, emitting light. The luminescent signal generated is proportional to the amount of ATP present, which correlates with the number of viable cells.
Lab products found in correlation
5 protocols using celltiter glo luminescent cell viability assay atp
Cell Viability and Apoptosis Assays with Sterile Particles
Cytotoxicity Evaluation of Compounds
Antibody and Chemical Reagents in Cell Studies
HUVEC Culture and VEGF Aptamer Analysis
Cytotoxicity and Cell Viability Assays
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