The largest database of trusted experimental protocols

Celltiter glo luminescent cell viability assay atp

Manufactured by Promega
Sourced in United States

The CellTiter-Glo® Luminescent Cell Viability Assay is a quantitative method for determining the number of viable cells in a cell-based assay. The assay measures the amount of ATP present, which is an indicator of the metabolically active cells. The assay uses a proprietary thermostable luciferase to catalyze the conversion of luciferin and ATP to oxyluciferin, emitting light. The luminescent signal generated is proportional to the amount of ATP present, which correlates with the number of viable cells.

Automatically generated - may contain errors

5 protocols using celltiter glo luminescent cell viability assay atp

1

Cell Viability and Apoptosis Assays with Sterile Particles

Check if the same lab product or an alternative is used in the 5 most similar protocols
Cell viability experiments were carried out in a 96-well format with 4 replicates. Cells were plated at a density of 5 × 104 cells per well. At 24 h following cell seeding, cells were treated with L-, D-, or DL-SPs (4.3 × 108 particles/mL) for 48 hr at 37°C in a 5% CO2 atmosphere and then assayed by using the ATP CellTiter-Glo® luminescent cell viability assay (Promega, Madison, WI) according to the manufacturer’s protocol. Luminescence intensity was measured using a microplate reader (Infinite M200, Tecan, Austria).
For assays investigating apoptosis inhibition, cells were incubated with 20 μM Z-VAD-fmk for 4 hr prior to the addition of SPs. After being treated with L-, D-, and DL-SPs (1.7 × 108 particles/mL) for 48 hr, cell viability assay was measured using the ATP CellTiter-Glo® luminescent cell viability assay.
+ Open protocol
+ Expand
2

Cytotoxicity Evaluation of Compounds

Check if the same lab product or an alternative is used in the 5 most similar protocols
The ATP CellTiter-Glo Luminescent Cell Viability Assay (Promega, Madison, WI) was used to measure cytotoxicity induced by the compounds in HCO cells and human aortic vascular smooth muscle cells (HAVSMC) (ATCC, Manassas, VA). HCO and HAVSMC cells were seeded in 96-well plates at 3000 and 7000 cells per well respectively. Cells were incubated overnight, treated with compound or DMSO vehicle and incubated for 24 h at 37 °C, 5% CO2. After incubation, plates were equilibrated for 30 min at room temperature and an equal volume of CellTiter-Glo Reagent was added. Plates were shaken for 2 min, incubated at room temperature for 10 min and the luminescent signal was read on a CLARIOstar plate reader.
+ Open protocol
+ Expand
3

Antibody and Chemical Reagents in Cell Studies

Check if the same lab product or an alternative is used in the 5 most similar protocols
The antibodies used were monoclonal GFP (Roche Applied Science, Indianapolis, IN), monoclonal actin (clone C4; Abcam, Cambridge, MA), polyclonal actin (Sigma-Aldrich), polyclonal Gaussia luciferase (New England Biolabs), polyclonal BiP (Cell Signaling, Danvers, MA), monoclonal FLAG (clone M2; Sigma-Aldrich), and monoclonal Myc (clone 4A6; Millipore). Chemicals used (with diluent in parentheses) were thapsigargin (dimethyl sulfoxide [DMSO] in in vitro studies, ethanol in in vivo studies; Sigma-Aldrich), cycloheximide (DMSO; Sigma-Aldrich), brefeldin A (ethanol; Sigma-Aldrich), dithiothreitol (water; Sigma-Aldrich), tunicamycin (DMSO; Sigma-Aldrich), cyclopiazonic acid (DMSO; Sigma-Aldrich), A23187 (DMSO; Sigma-Aldrich), TPEN (DMSO; Sigma-Aldrich), dantrolene (DMSO; Sigma-Aldrich), xestospongin C (DMSO; Sigma-Aldrich), caffeine (water; Sigma-Aldrich), glutamate (1 N HCl; Sigma-Aldrich), celecoxib (DMSO; Sigma-Aldrich), valdecoxib (DMSO; Sigma-Aldrich), and 2,5-dimethyl-celecoxib (DMSO; Sigma-Aldrich). Vehicle controls at concentrations equivalent to treatments were used in all experiments. Cell viability assays were performed with the CellTiter 96 AQueous One Solution Cell Proliferation Assay (MTS) or the CellTiter-Glo Luminescent Cell Viability Assay (ATP) following the manufacturer's instructions (Promega, Madison, WI).
+ Open protocol
+ Expand
4

HUVEC Culture and VEGF Aptamer Analysis

Check if the same lab product or an alternative is used in the 5 most similar protocols
Human Umbilical Vein Endothelial Cells (HUVECs) were purchased from ATCC (Manassas, VA). Medium 200 (M200), low serum growth supplement (LSGS), bovine serum albumin (BSA), fetal bovine serum (FBS), Geltrex, and calcein AM were purchased from ThermoFisher (Waltham, MA). VEGF-165 (VEGF, MW= 38.2 kDa) and VEGF enzyme-linked immunosorbent assay (ELISA) kit were obtained from Peprotech (Rocky Hill, NJ). CellTiter-Glo® Luminescent Cell Viability Assay (ATP) were obtained from Promega (Madison, WI). Acrydite modified VEGF (MW ~10 kDa) and c-MET (MW ~17.5 kDa) aptamers, and their complementary sequence (CS) were custom ordered from Integrated DNA technologies (Coralville, PA).
+ Open protocol
+ Expand
5

Cytotoxicity and Cell Viability Assays

Check if the same lab product or an alternative is used in the 5 most similar protocols
The Beta-Glo® Assay System, CytoTox 96® Non-Radioactive Cytotoxicity Assay, GSH-Glo™ Glutathione Assay, CellTiter-Glo® Luminescent Cell Viability Assay (ATP) and CellTiter 96® AQueous One Solution Cell Proliferation Assay (MTS) were purchased from Promega (Madison, WI, USA); graphite flakes were provided by Asbury Graphite Mills; Hoechst 33342, FITC labeled Bovine Serum Albumin (BSA), Alexa Fluor 594-conjugated wheat germ agglutinin (WGA), Propidium iodide and Image-iT® Lipid Peroxidation Kit were purchased from Life Technologies (Grand Island, NY, USA). Min-U-Sil was obtained from U.S. Silica (Frederick, MD, USA). Bronchial epithelial growth medium (BEGM) was obtained from Lonza (Mapleton, IL, USA): this medium is supplemented with a number of growth factors, including bovine pituitary extract (BPE), insulin, hydrocortisone, hEGF, epinephrine, triiodothyronine, transferrin, gentamicin/amphotericin-B and retinoic acid. Roswell Park Memorial Institute medium 1640 (RPMI 1640) was purchased from Invitrogen (Carlsbad, CA, USA). Low-endotoxin bovine serum albumin (BSA) and fetal bovine serum (FBS) were purchased from Gemini Bio-Products (West Sacramento, CA, USA).
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!