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Celltiter glo luminescent cell viability assay atp

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The CellTiter-Glo® Luminescent Cell Viability Assay is a quantitative method for determining the number of viable cells in a cell-based assay. The assay measures the amount of ATP present, which is an indicator of the metabolically active cells. The assay uses a proprietary thermostable luciferase to catalyze the conversion of luciferin and ATP to oxyluciferin, emitting light. The luminescent signal generated is proportional to the amount of ATP present, which correlates with the number of viable cells.

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Lab products found in correlation

Celltiter 96 aqueous one solution cell proliferation assay mts, by Promega (2 mentions) Fetal bovine serum (fbs), by Thermo Fisher Scientific (2 mentions) Infinite m200, by Tecan (1 mentions) Cyclopiazonic acid, by Merck Group (1 mentions) Dantrolene, by Merck Group (1 mentions) Valdecoxib, by Merck Group (1 mentions) Glutamate, by Merck Group (1 mentions) Celecoxib, by Merck Group (1 mentions) Xestospongin c, by Merck Group (1 mentions) Monoclonal flag clone m2, by Merck Group (1 mentions) 2 5 dimethyl celecoxib, by Merck Group (1 mentions) Brefeldin a, by Merck Group (1 mentions) Caffeine, by Merck Group (1 mentions) Cycloheximide (chx), by Merck Group (1 mentions) A23187, by Merck Group (1 mentions) Dithiothreitol (dtt), by Merck Group (1 mentions) Thapsigargin, by Merck Group (1 mentions) Tunicamycin, by Merck Group (1 mentions) Vegf enzyme linked immunosorbent assay elisa kit, by Thermo Fisher Scientific (1 mentions) Geltrex, by Thermo Fisher Scientific (1 mentions)

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CellTiter-Glo luminescent CellTiter-Glo assay CellTiter-Glo Luminescent assays

5 protocols using celltiter glo luminescent cell viability assay atp

1

Cell Viability and Apoptosis Assays with Sterile Particles

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Cell viability experiments were carried out in a 96-well format with 4 replicates. Cells were plated at a density of 5 × 104 cells per well. At 24 h following cell seeding, cells were treated with L-, D-, or DL-SPs (4.3 × 108 particles/mL) for 48 hr at 37°C in a 5% CO2 atmosphere and then assayed by using the ATP CellTiter-Glo® luminescent cell viability assay (Promega, Madison, WI) according to the manufacturer’s protocol. Luminescence intensity was measured using a microplate reader (Infinite M200, Tecan, Austria).
For assays investigating apoptosis inhibition, cells were incubated with 20 μM Z-VAD-fmk for 4 hr prior to the addition of SPs. After being treated with L-, D-, and DL-SPs (1.7 × 108 particles/mL) for 48 hr, cell viability assay was measured using the ATP CellTiter-Glo® luminescent cell viability assay.
Yeom J., Guimaraes P.P., Ahn H.M., Jung B., Hu Q., McHugh K., Mitchell M.J., Yun C.O., Langer R, & Jaklenec A. (2019). Chiral Effects on Supraparticles for Controllable Nanomedicine. Advanced materials (Deerfield Beach, Fla.), 32(1), e1903878.
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Cytotoxicity Evaluation of Compounds

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The ATP CellTiter-Glo Luminescent Cell Viability Assay (Promega, Madison, WI) was used to measure cytotoxicity induced by the compounds in HCO cells and human aortic vascular smooth muscle cells (HAVSMC) (ATCC, Manassas, VA). HCO and HAVSMC cells were seeded in 96-well plates at 3000 and 7000 cells per well respectively. Cells were incubated overnight, treated with compound or DMSO vehicle and incubated for 24 h at 37 °C, 5% CO2. After incubation, plates were equilibrated for 30 min at room temperature and an equal volume of CellTiter-Glo Reagent was added. Plates were shaken for 2 min, incubated at room temperature for 10 min and the luminescent signal was read on a CLARIOstar plate reader.
Esteban-Lopez M., Wilson K.J., Myhr C., Kaftanovskaya E.M., Henderson M.J., Southall N.T., Xu X., Wang A., Hu X., Barnaeva E., Ye W., George E.R., Sherrill J.T., Ferrer M., Morello R., Agoulnik I.U., Marugan J.J, & Agoulnik A.I. (2022). Discovery of small molecule agonists of the Relaxin Family Peptide Receptor 2. Communications Biology, 5, 1183.
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3

Antibody and Chemical Reagents in Cell Studies

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The antibodies used were monoclonal GFP (Roche Applied Science, Indianapolis, IN), monoclonal actin (clone C4; Abcam, Cambridge, MA), polyclonal actin (Sigma-Aldrich), polyclonal Gaussia luciferase (New England Biolabs), polyclonal BiP (Cell Signaling, Danvers, MA), monoclonal FLAG (clone M2; Sigma-Aldrich), and monoclonal Myc (clone 4A6; Millipore). Chemicals used (with diluent in parentheses) were thapsigargin (dimethyl sulfoxide [DMSO] in in vitro studies, ethanol in in vivo studies; Sigma-Aldrich), cycloheximide (DMSO; Sigma-Aldrich), brefeldin A (ethanol; Sigma-Aldrich), dithiothreitol (water; Sigma-Aldrich), tunicamycin (DMSO; Sigma-Aldrich), cyclopiazonic acid (DMSO; Sigma-Aldrich), A23187 (DMSO; Sigma-Aldrich), TPEN (DMSO; Sigma-Aldrich), dantrolene (DMSO; Sigma-Aldrich), xestospongin C (DMSO; Sigma-Aldrich), caffeine (water; Sigma-Aldrich), glutamate (1 N HCl; Sigma-Aldrich), celecoxib (DMSO; Sigma-Aldrich), valdecoxib (DMSO; Sigma-Aldrich), and 2,5-dimethyl-celecoxib (DMSO; Sigma-Aldrich). Vehicle controls at concentrations equivalent to treatments were used in all experiments. Cell viability assays were performed with the CellTiter 96 AQueous One Solution Cell Proliferation Assay (MTS) or the CellTiter-Glo Luminescent Cell Viability Assay (ATP) following the manufacturer's instructions (Promega, Madison, WI).
Henderson M.J., Wires E.S., Trychta K.A., Richie C.T, & Harvey B.K. (2014). SERCaMP: a carboxy-terminal protein modification that enables monitoring of ER calcium homeostasis. Molecular Biology of the Cell, 25(18), 2828-2839.
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4

HUVEC Culture and VEGF Aptamer Analysis

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Human Umbilical Vein Endothelial Cells (HUVECs) were purchased from ATCC (Manassas, VA). Medium 200 (M200), low serum growth supplement (LSGS), bovine serum albumin (BSA), fetal bovine serum (FBS), Geltrex, and calcein AM were purchased from ThermoFisher (Waltham, MA). VEGF-165 (VEGF, MW= 38.2 kDa) and VEGF enzyme-linked immunosorbent assay (ELISA) kit were obtained from Peprotech (Rocky Hill, NJ). CellTiter-Glo® Luminescent Cell Viability Assay (ATP) were obtained from Promega (Madison, WI). Acrydite modified VEGF (MW ~10 kDa) and c-MET (MW ~17.5 kDa) aptamers, and their complementary sequence (CS) were custom ordered from Integrated DNA technologies (Coralville, PA).
Abune L., Lee K, & Wang Y. (2022). Development of biomimetic extracellular matrix with the functions of protein sequestration and cell attachment using dual aptamer-functionalized hydrogels. ACS biomaterials science & engineering, 8(3), 1279-1289.
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5

Cytotoxicity and Cell Viability Assays

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The Beta-Glo® Assay System, CytoTox 96® Non-Radioactive Cytotoxicity Assay, GSH-Glo™ Glutathione Assay, CellTiter-Glo® Luminescent Cell Viability Assay (ATP) and CellTiter 96® AQueous One Solution Cell Proliferation Assay (MTS) were purchased from Promega (Madison, WI, USA); graphite flakes were provided by Asbury Graphite Mills; Hoechst 33342, FITC labeled Bovine Serum Albumin (BSA), Alexa Fluor 594-conjugated wheat germ agglutinin (WGA), Propidium iodide and Image-iT® Lipid Peroxidation Kit were purchased from Life Technologies (Grand Island, NY, USA). Min-U-Sil was obtained from U.S. Silica (Frederick, MD, USA). Bronchial epithelial growth medium (BEGM) was obtained from Lonza (Mapleton, IL, USA): this medium is supplemented with a number of growth factors, including bovine pituitary extract (BPE), insulin, hydrocortisone, hEGF, epinephrine, triiodothyronine, transferrin, gentamicin/amphotericin-B and retinoic acid. Roswell Park Memorial Institute medium 1640 (RPMI 1640) was purchased from Invitrogen (Carlsbad, CA, USA). Low-endotoxin bovine serum albumin (BSA) and fetal bovine serum (FBS) were purchased from Gemini Bio-Products (West Sacramento, CA, USA).
Li R., Guiney L.M., Chang C.H., Mansukhani N.D., Ji Z., Wang X., Liao Y.P., Jiang W., Sun B., Hersam M.C., Nel A.E, & Xia T. (2018). The Surface Oxidation of Graphene Oxide Determines Membrane Damage, Lipid Peroxidation, and Cytotoxicity in Macrophages in a Pulmonary Toxicity Model. ACS nano, 12(2), 1390-1402.
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