Ginsenoside rb1

Ginsenoside Rb1 was purchased from Beijing Solarbio Technology Co., Ltd. (Beijing, China) with product number SA9790 and purity ≥ 98%. Influenza A virus antigen and IIV4 were purchased from Zhejiang Tianyuan Biopharmaceutical Co., Ltd; the A/Michigan/45/2015 virus was stored in our laboratory. Aluminum hydroxide adjuvant was purchased from Thermo Fisher Scientific Co., Ltd (Waltham, MA, USA). Secondary antibodies were purchased from Southern Biotech. The cytokine kit was purchased from Beijing 4A Biotech Co., Ltd.

The five chemical components of SLBZS were analyzed by a Waters 1525 high-performance liquid chromatography (HPLC) system. SLBZS components were separated by a Sunfire C18 (4.6 mm × 250 mm, 5 μm). For each run, 10 μl was taken, and the flow rate was 1.0 ml/min. Ginsenoside Rg1(Solarbio, 724B021), ginsenoside Re (Solarbio, 717B021), ginsenoside Rb1(Solarbio, 727C021), liquiritin (Meilunbio, A0802AS), and atractylenolide III (Meilunbio, O1021AS) were selected as the standard products and were detected at a wavelength of 203 nm. The mobile phase was 0.05% phosphoric acid (A) and acetonitrile (B). The gradient procedure was as follows: 79% for 0 to 30 min; 79% to 71% for 30 to 50 min; 71% for 50 to 70 min; 71% to 52% for 70 to 100 min; and 52% to 52% for 100 to 130 min.