Log In Sign up
The largest database of trusted experimental protocols

Sf3b1

Manufactured by Fortis Life Sciences
Visit Supplier

The SF3B1 is a protein that plays a critical role in the spliceosome, a complex molecular machinery responsible for the removal of introns from pre-mRNA during the process of gene expression. This protein is essential for the proper assembly and functioning of the spliceosome, which is a fundamental step in the gene expression pathway.

Automatically generated - may contain errors

Lab products found in correlation

U2af1, by Fortis Life Sciences (3 mentions) Flag tag (flag), by Merck Group (3 mentions) Vinculin, by Merck Group (2 mentions) Snrpf, by Abcam (2 mentions) Bud31, by Proteintech (2 mentions) Prp19, by Fortis Life Sciences (2 mentions) Sf3a1, by Fortis Life Sciences (2 mentions) Eftud2, by Fortis Life Sciences (2 mentions) Eif2s1, by Abcepta (2 mentions) Eif3i p36, by BioLegend (2 mentions) C myc, by Cell Signaling Technology (2 mentions) Cleaved caspase 3, by Cell Signaling Technology (2 mentions) Alexa fluor 568 donkey anti mouse igg, by Thermo Fisher Scientific (1 mentions) Actin clone c4, by MP Biomedicals (1 mentions) Alexa fluor 488 donkey anti rabbit igg, by Thermo Fisher Scientific (1 mentions) β actin c4, by Santa Cruz Biotechnology (1 mentions) Eif2α, by Cell Signaling Technology (1 mentions) Phospho eif2α ser51, by Cell Signaling Technology (1 mentions) Phospho 4e bp1 thr37 46, by Cell Signaling Technology (1 mentions) 4e bp1, by Cell Signaling Technology (1 mentions)

Close spelling variants of this product

Anti-SF3B1 (4 citations)

5 protocols using sf3b1

1

Comprehensive Protein Analysis by Western Blot

Check if the same lab product or an alternative is used in the 5 most similar protocols
Cells were lysed in 1× SDS sample buffer (62.5mM Tris-HCl, pH6.8, 10% glycerol, 2% SDS, 2.5% β-mercaptoethanol) and heated at 95°C for 12 minutes. The following antibodies were used for Western blotting: Flag (Sigma, A8592), BUD31 (ProteinTech, 11798-1-AP), SF3B1 (Bethyl, A300-996A), Prp19 (Bethyl, A300-101A), U2AF1 (Bethyl, A302-079A), SF3A1 (Bethyl, A301-603A), EFTUD2 (Bethyl, A300-957A), SNRPF (Abcam, 154870), HER2 (Millipore, 06-562), EGFR (Cell Signaling, 2232), cleaved caspase-3 (Cell Signaling, 9664), RPS8 (Assay Biotechnology, R12-3466), EIF2S1 (Abgent, AP13469s), eIF3I (p36) (Biolegend, 646701), and c-Myc (D84C12) (Cell Signaling, 5605). Vinculin (Sigma, V9131) and ran (BD Biosciences, 610340) was used as loading control.
Hsu T.Y., Simon L.M., Neill N., Marcotte R., Sayad A., Bland C.S., Echeverria G.V., Sun T., Kurley S.J., Tyagi S., Karlin K.L., Dominguez-Vidaña R., Hartman J.D., Renwick A., Scorsone K., Bernardi R.J., Skinner S.O., Jain A., Orellana M., Lagisetti C., Golding I., Jung S.Y., Neilson J.R., Zhang X.H., Cooper T.A., Webb T.R., Neel B.G., Shaw C.A, & Westbrook T.F. (2015). The spliceosome is a therapeutic vulnerability in MYC-driven cancer. Nature, 525(7569), 384-388.
+ Open protocol
+ Expand
2

Immunostaining of Spliceosome Proteins

Check if the same lab product or an alternative is used in the 5 most similar protocols
The following antibodies were used: ACTIN (clone C4, MP Biomedicals), PRPF6 (Bethyl Laboratories), PRPF31 (clone 8E1, Abnova), PRPF4 (Abnova), BRR2 (Sigma), PRPF8 (ProteinTech), SNU114 (Novus), PRPF3 (Sigma), SF3B1 (Bethyl Laboratories), SF3B2 (Bethyl Laboratories), SNRNP70 (Sigma), Alexa Fluor 488 donkey anti-rabbit IgG (Invitrogen), and Alexa Fluor 568 donkey anti-mouse IgG (Invitrogen).
Adler A.S., McCleland M.L., Yee S., Yaylaoglu M., Hussain S., Cosino E., Quinones G., Modrusan Z., Seshagiri S., Torres E., Chopra V.S., Haley B., Zhang Z., Blackwood E.M., Singh M., Junttila M., Stephan J.P., Liu J., Pau G., Fearon E.R., Jiang Z, & Firestein R. (2014). An integrative analysis of colon cancer identifies an essential function for PRPF6 in tumor growth. Genes & Development, 28(10), 1068-1084.
+ Open protocol
+ Expand
3

Validating Splicing Factor Interactions with DNMT3A

Check if the same lab product or an alternative is used in the 5 most similar protocols
Splicing factor interactions were validated after exogenous expression of pCAG containing myc-tagged DNMT3A into HEK293T cells. Pierce anti-myc magnetic beads (Invitrogen 88843) were used to immunoprecipitate myc-tagged DNMT3A and associated proteins. The co-IP protocol was as described by the manufacturer. Binding buffer consisted of 25mM Tris-HCl pH. 8.0, 150 mM NaCl, 0.05% Tween-20. Wash buffer consisted of 125mM Tris-HCl pH. 8.0, 750mM NaCl, 0.25% Tween-20.
Cells were lysed in CytoBuster Protein Extraction Reagent for 20 minutes at room temperature. Soluble protein fraction was isolated after centrifugation at 14,000 RPM for 5 minutes at 4°C. 1X Laemlli Sample Buffer (Biorad, 161-0747) was added in a 1:1 ratio and heated at 95°C for 15 min. Eluates were run on 4-15% precast gels (Biorad 4561084) and transferred following standard western blot protocols. The following antibodies were used for western blotting: Flag (Sigma, F1804), SF3B1 (Bethyl, A300-996A), SF3B3 (Bethyl, A302-079A), DDX39B (H6) (Santa Cruz, sc-271395), c-Myc tag (9E10) (Thermo, 13-2500). β-actin (C4) (Santa Cruz, sc-47778) was used as a loading control.
Ramabadran R., Wang J.H., Reyes J.M., Guzman A.G., Gupta S., Rosas C., Brunetti L., Gundry M.C., Tovy A., Long H., Gu T., Cullen S.M., Tyagi S., Rux D., Kim J.J., Kornblau S.M., Kyba M., Stossi F., Rau R.E., Takahashi K., Westbrook T.F, & Goodell M.A. (2023). DNMT3A-coordinated splicing governs the stem state switch toward differentiation in embryonic and hematopoietic stem cells. Nature cell biology, 25(4), 528-539.
+ Open protocol
+ Expand
4

Comprehensive Protein Analysis by Western Blot

Check if the same lab product or an alternative is used in the 5 most similar protocols
Cells were lysed in 1× SDS sample buffer (62.5mM Tris-HCl, pH6.8, 10% glycerol, 2% SDS, 2.5% β-mercaptoethanol) and heated at 95°C for 12 minutes. The following antibodies were used for Western blotting: Flag (Sigma, A8592), BUD31 (ProteinTech, 11798-1-AP), SF3B1 (Bethyl, A300-996A), Prp19 (Bethyl, A300-101A), U2AF1 (Bethyl, A302-079A), SF3A1 (Bethyl, A301-603A), EFTUD2 (Bethyl, A300-957A), SNRPF (Abcam, 154870), HER2 (Millipore, 06-562), EGFR (Cell Signaling, 2232), cleaved caspase-3 (Cell Signaling, 9664), RPS8 (Assay Biotechnology, R12-3466), EIF2S1 (Abgent, AP13469s), eIF3I (p36) (Biolegend, 646701), and c-Myc (D84C12) (Cell Signaling, 5605). Vinculin (Sigma, V9131) and ran (BD Biosciences, 610340) was used as loading control.
Hsu T.Y., Simon L.M., Neill N., Marcotte R., Sayad A., Bland C.S., Echeverria G.V., Sun T., Kurley S.J., Tyagi S., Karlin K.L., Dominguez-Vidaña R., Hartman J.D., Renwick A., Scorsone K., Bernardi R.J., Skinner S.O., Jain A., Orellana M., Lagisetti C., Golding I., Jung S.Y., Neilson J.R., Zhang X.H., Cooper T.A., Webb T.R., Neel B.G., Shaw C.A, & Westbrook T.F. (2015). The spliceosome is a therapeutic vulnerability in MYC-driven cancer. Nature, 525(7569), 384-388.
+ Open protocol
+ Expand
5

Molecular Characterization of Cellular Machinery

Check if the same lab product or an alternative is used in the 5 most similar protocols
The following antibodies and plasmids were used where indicated. SF3B1 (Bethyl Laboratories A300-997A), SDE2 (Bethyl Laboratories A302-098A and A302-099A), GAPDH (Santa Cruz Biotechnologies sc-47724), U2AF1 (Bethyl Laboratories A302-079A), CUL7 (Bethyl Laboratories A300-223A), PITPNM1 (Abcam AB254959), Puromycin (EMD Millipore MABE343), Histone 4 (Active Motif #39269), eIF2α (Cell Signaling Technology 9722S), Phospho- eIF2α−Ser51 (Cell Signaling Technology 9721S), 4E-BP1 (Cell Signaling Technology 9644S), Phospho-4E-BP1-Thr37/46 (Cell Signaling Technology 2855S), Cactin (Bethyl Laboratories A303-349A), FBL (Abcam ab5821), Tubulin (Cell Signaling Technology 2125S), p54 (Santa Cruz Biotechnology sc-126) and p21 (Santa Cruz Biotechnology sc-6246). The following plasmids were used: pLKO.1.
Floro J., Dai A., Metzger A., Mora-Martin A., Ganem N.J., Cifuentes D., Wu C.S., Dalal J., Lyons S.M., Labadorf A, & Flynn R.L. (2021). SDE2 is an essential gene required for ribosome biogenesis and the regulation of alternative splicing. Nucleic Acids Research, 49(16), 9424-9443.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!