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Protease inhibitor cocktail tablets edta free

Manufactured by Merck Group
Sourced in United States

Protease Inhibitor Cocktail Tablets EDTA-free is a laboratory product manufactured by Merck Group. It is a mixture of protease inhibitors designed to prevent the degradation of proteins during sample preparation and analysis.

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2 protocols using protease inhibitor cocktail tablets edta free

1

HUWE1 Gene Silencing Protocol

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Dimethyloxallyl glycine and MG132 were purchased from Cayman. CHX, Protease Inhibitor Cocktail Tablets EDTA-free, [13C6,15N2]-l-lysine, [13C6,15N4]-l-arginine, l-lysine, and l-arginine were purchased from Sigma-Aldrich. Lipofectamine RNAiMAX was purchased from Invitrogen. HUWE1 siRNA On-TARGETplus SMARTpools and negative control siRNA were purchased from Dharmacon. Benzonase nuclease lyophilized powder (CAS 9025-65-4) was purchased from Santa Cruz.
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2

Protein and DNA Marker Acquisition Protocol

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For the protein standards, we obtained the following from GE Healthcare (Chicago, IL, USA): PageRuler™ Plus Prestained Protein Ladder (catalog number 26619), LMW SDS Marker Kit (catalog number 17044601), and Coomassie™ Brilliant Blue (catalog number 20279). The DNA markers were acquired from Thermo Fisher Scientific (Waltham, MA, USA) and GE Healthcare Bio-Sciences AB (Piscataway, NJ, USA). The protease inhibitor used in the study was Protease Inhibitor Cocktail Tablets, EDTA-Free (catalog number S8820), obtained from Sigma-Aldrich (St. Louis, MO, USA). Sephacryl S300 HR and all other necessary chemicals were also acquired from Sigma-Aldrich (St. Louis, MO, USA).
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