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Ix81 microscope

Manufactured by Molecular Devices
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The IX81 microscope is a high-performance inverted microscope designed for a wide range of biological and biomedical applications. It features a modular and flexible design, allowing for easy customization and integration with various accessories and imaging techniques.

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Lab products found in correlation

Metamorph software, by Molecular Devices (2 mentions) Ix81 fluorescent microscope, by Olympus (1 mentions) Metamorph software, by Olympus (1 mentions)

3 protocols using ix81 microscope

1

Extracellular Integrin Quantification

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Cells plated on collagen-coated coverslips were fixed but not permeabilized and subsequently incubated with integrin antibodies directed against extracellular domains. Samples were then prepared according to our standard IF protocol (above) and imaged on an Olympus IX81 microscope controlled by Metamorph software Molecular Devices) using a 0.30 N.A. 10x objective. Cells were outlined in ImageJ and integrated pixel density is reported (in A.U.).
Rotty J.D., Brighton H.E., Craig S.L., Asokan S.B., Cheng N., Ting J.P, & Bear J.E. (2017). Arp2/3 complex is required for macrophage integrin functions but is dispensable for FcR phagocytosis and in vivo motility. Developmental cell, 42(5), 498-513.e6.
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2

Quantitative F-Actin Imaging and Analysis

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After staining with fluorescent phalloidin, images were taken on an Olympus IX81 microscope controlled by Metamorph software (Molecular Devices) using a 0.30 N.A. 10x objective. Phalloidin images were imported to ImageJ and background subtracted. Cells were outlined by hand and integrated pixel density (i.e. F-actin staining), cell area and cell perimeter were measured on a per cell basis. Integrated densities are reported in arbitrary units (A.U.). Spread cell area was directly reported based on these measurements. Cell shape was determined quantitatively with the following equation: (4π*cell area)/(cell perimeter2). A circle would have a value of 1, while a straight line would have a value of 0.
Rotty J.D., Brighton H.E., Craig S.L., Asokan S.B., Cheng N., Ting J.P, & Bear J.E. (2017). Arp2/3 complex is required for macrophage integrin functions but is dispensable for FcR phagocytosis and in vivo motility. Developmental cell, 42(5), 498-513.e6.
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3

Histological Analysis of Iron Deposition and Oxidative Stress in Mouse Hearts

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Mouse hearts were removed and arrested in diastole by using 1 mol/L KCl, fixed with 10% buffered formalin, and embedded in paraffin. Thin sections (5 μm) were used for Prussian blue, picro‐sirius red (PSR), and Masson trichrome staining as described previously.30, 38 Briefly, tissue sections were deparaffinized in xylene and alcohol grades, rehydrated in water, and subjected to staining as described previously.30 The deposition of iron was visualized as blue depositions using bright‐field microscopy. Myocardial collagen content was evaluated by using PSR staining and visualization using an Olympus IX81 microscope and image analysis using MetaMorph software (Molecular Devices, Sunnyvale, CA) as described previously.30, 35, 38 4‐Hydroxynonenal (4‐HNE) immunofluorescence detected an important marker of iron‐induced lipid peroxidation as previously described.30 We also performed dihydroethidium (DHE) and dichlorodihydrofluorescein (DCF) fluorescence staining as described previously,19, 35 which were visualized using an Olympus IX81 fluorescent microscope and quantified using the MetaMorph software.
Das S.K., Patel V.B., Basu R., Wang W., DesAulniers J., Kassiri Z, & Oudit G.Y. (2017). Females Are Protected From Iron‐Overload Cardiomyopathy Independent of Iron Metabolism: Key Role of Oxidative Stress. Journal of the American Heart Association: Cardiovascular and Cerebrovascular Disease, 6(1), e003456.
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