Smooth muscle actin (SMA) expression in explanted cells was analyzed by immunofluorescence. SMA primary antibody (Sigma A2547, dilution 1:500) was diluted in 2% bovine serum albumin (BSA; EMD Chemicals, San Diego, CA) in PBS. Primary antibodies were detected using 1:500 Alexa Fluor® 568 conjugated mouse secondary antibody (Life Technologies A11004, Waltham, MA) and nuclei were detected with 1:10,000 4’,6-diamidino-2-phenylindole,dihydrochloride (DAPI; Life Technologies D1306) diluted in 2% BSA in PBS. Imaging was performed on a Leica SP8 confocal microscope (Wetzlar, Germany) or a Leica DMIRB epi-fluorescent microscope (Wetzlar, Germany).
Sma primary antibody
Manufactured by Merck Group
SMA primary antibody is a laboratory reagent used in immunochemical techniques to detect the presence of smooth muscle actin (SMA), a protein found in the contractile apparatus of smooth muscle cells. It is commonly used as a marker for the identification and characterization of smooth muscle cells in various tissues and cell cultures.
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Lab products found in correlation
2 protocols using sma primary antibody
1
Immunofluorescence Analysis of Smooth Muscle Actin
2
Immunofluorescence Analysis of Smooth Muscle Actin
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