Avance 3 hd 500 mhz nmr

Manufactured by Bruker

Sourced in United States

The AVANCE III HD 500 MHz NMR is a high-performance nuclear magnetic resonance (NMR) spectrometer designed for laboratory use. It operates at a frequency of 500 MHz and is capable of performing various NMR experiments to analyze the chemical structure and properties of samples.

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Lab products found in correlation

Qci cryoprobe, by Bruker (1 mentions) Tsp d4, by Merck Group (1 mentions) Tsp d4, by Bruker (1 mentions) Anhydrous dmf, by Thermo Fisher Scientific (1 mentions) Dimethyl sulfoxide (dmso), by Thermo Fisher Scientific (1 mentions) Nmr solvents, by Cambridge Isotopes (1 mentions) Ch2cl2, by MBraun (1 mentions) Ch3oh, by Thermo Fisher Scientific (1 mentions) Combiflash rf 200, by Teledyne (1 mentions)

Close spelling variants of this product

Avance III (981 citations) Avance III HD (325 citations) Avance 500 (319 citations) Avance III 500 (190 citations) Avance III 500 MHz (142 citations) Avance 500 MHz (103 citations) Avance III HD 500 (68 citations) Avance III HD 500 MHz (42 citations) 500 MHz NMR (28 citations) Avance III HD 500 MHz NMR spectrometer (19 citations)

3 protocols using avance 3 hd 500 mhz nmr

NMR Analysis of RAM 2-45 Protein

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NMR data was acquired on a Bruker AVANCE III HD 500 MHz NMR spectrometer equipped with a 5 mm QCI-F probe. The ¹H–¹⁵N HSQC included suppression of the water by the WATERGATE pulse sequence (27 (link),28 ). ¹⁵N-labeled RAM 2–45 protein was expressed in Escherichia coli BL21 Codon Plus RIL strain grown in minimal medium supplemented with ¹⁵NH₄CL and purified as for X-ray crystallography at a concentration of 230 μM in 25 mM PIPES (pH 6.5, 175 mM NaCl, 25 mM KCl, 10% glycerol, 1 mM TCEP and 10% D2O). RMNT 165–476 (unlabeled)—RAM 2–45 (¹⁵N-labeled) complex was prepared as described for X-ray crystallography at a concentration of 160 μM in buffer conditions identical to monomeric RAM. The sample volume was 200 μl in a 3 mm NMR tube and temperature of the sample during acquisition was 25°C.

Varshney D., Petit A.P., Bueren-Calabuig J.A., Jansen C., Fletcher D.A., Peggie M., Weidlich S., Scullion P., Pisliakov A.V, & Cowling V.H. (2016). Molecular basis of RNA guanine-7 methyltransferase (RNMT) activation by RAM. Nucleic Acids Research, 44(21), 10423-10436.

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Structural Elucidation of LAK2 via NMR

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LAK2 (25 mg as formate) was dissolved in 0.6 mL of deuterium oxide containing 0.05% 3-(trimethylsilyl)propionic-2,2,3,3-d₄ acid, sodium salt (TSP-d₄, Sigma-Aldrich). Nuclear magnetic resonance (NMR) measurements were performed using an AVANCE III HD 500 MHz NMR spectrometer equipped with a QCI CryoProbe (Bruker, Billerica, MA, USA) at 298 K. The ¹H- and ¹³C-NMR chemical shifts were recorded relative to the internal reference TSP-d₄. The molecular structure of LAK2 was determined based on the NMR spectra of one-dimensional (1D)-¹H, 1D-¹³C, ¹H–¹H COSY, NOESY, ¹H–¹³C edited-HSQC, ¹H–¹³C HMBC, 1,1-ADEQUATE, and ¹H–¹⁵N HMBC. Instrument operation, data processing, and data analysis were performed using the TopSpin software 3.6 (Bruker).

Shigeta T., Sasamoto K, & Yamamoto T. (2020). A novel crosslinked type of advanced glycation end-product derived from lactaldehyde. Heliyon, 6(11), e05337.

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Detailed Analytical Procedures for Chemical Synthesis

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All chemicals were obtained from commercial sources and used without further
purification unless otherwise noted. Anhydrous DMF, CH₃OH, DMSO and EtOH
were purchased from Fisher Scientific. Anhydrous THF, acetone, CH2Cl2, CH3CN, and
ether were obtained using a solvent purification system (mBraun Labmaster 130). NMR
solvents were purchased from Cambridge Isotope Laboratories (Andover, MA). All 1H,
13C, 19F and 31P NMR spectra were obtained either on a JEOL ECX 400 MHz NMR,
operated at 400 and 100 MHz, respectively, or a Bruker AVANCE III HD 500 MHz NMR,
operated at 500 and 125 MHz, respectively, and referenced to internal
tetramethylsilane (TMS) at 0.0 ppm. The spin multiplicities are indicated by the
symbols s (singlet), d (doublet), dd (doublet of doublets), t (triplet), q
(quartet), m (multiplet), and br (broad). Reactions were monitored by thin-layer
chromatography (TLC) using 0.25 mm Whatman Diamond silica gel 60-F254 pre-coated
plates. Purification was performed on a Teledyne Isco CombiFlash Rf 200,and eluted
with the indicated solvent system. Yields refer to chromatographically and
spectroscopically (¹H and ¹³C NMR) homogeneous materials. Mass
Spectra were recorded at the UMBC MCAC for nominal using Bruker APOLLO™ II
ESI/APCI - MALDI Dual Source for apex(R)-Qe FTMS or Johns Hopkins Mass Spectrometry
Facility for high resolution using VG Analytical VG-70SE Magnetic Sector Mass
Spectrometer.

Ku T., Lopresti N., Shirley M., Mori M., Marchant J., Heng X., Botta M., Summers M.F, & Seley-Radtke K.L. (2019). Synthesis of distal and proximal fleximer base analogues and evaluation in the nucleocapsid protein of HIV-1. Bioorganic & Medicinal Chemistry, 27(13), 2883-2892.

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