Anti β actin antibody mab1501

DHT, bicalutamide, cyclohexamide, tolbutamide, and minoxidil were obtained from Sigma Chemical Co. (St. Louis, MO). The cell lines PC-3, LNCaP, LAPC4, and HepG2 were purchased from American Type Culture Collection (Manassas, VA). Human hair dermal papillae cells (HHDPCs) were purchased from ScienCell Research Laboratories (San Diego, CA). The plasmids pSG5-AR, pSG5-GR, Gal-4-ARA54C, Gal-4-peptide, pCDNA3-AR-N, pCDNA3-AR-C, pCMX-VP16-AR, MMTV-Luc, PSA-Luc, and pG5-Luc were constructed as described previously [21 (link), 22 (link)]. Anti-AR (N-20) and anti-PSA (C-19) antibodies were purchased from Santa Cruz Biotechnology, Inc. (Santa Cruz, CA). The anti-β-actin antibody (MAB1501) was obtained from Millipore (Billerica, MA).

Antibodies were used to neutralize TRAIL signaling and for immunoblotting analysis. Anti-TRAIL antibody (B-T24) for neutralization was purchased from Diaclone (Besançon cedex, France). For immunoblotting analysis, antibodies against caspase 3 (9662), caspase 9 (9508), Bcl-2 (2876), Bcl-XL (2764), and Mcl-1 (5453) were purchased from Cell Signaling Technology (Danvers, MA, USA). Anti-caspase-8 (ALX-804-477) antibody was purchased from Enzo Life Sciences (Farmingdale, NY, USA) and anti-β-actin antibody (MAB1501) was purchased from Millipore (Thermo Fisher Scientific). Anti-XIAP antibody (sc-55,551) was purchased from Santa Cruz Biotechnology Inc. (Santa Cruz, CA, USA).