C 12212

Manufactured by PromoCell

Sourced in Germany

The C-12212 is a laboratory equipment item. It is a device used for a specific function in a laboratory setting. No further details about its core function or intended use are provided, as an unbiased and factual description cannot be given without the risk of extrapolation.

Automatically generated - may contain errors

Lab products found in correlation

C 12281, by PromoCell (1 mentions) Cc 3162, by Lonza (1 mentions) Axiovert 200m inverted microscope, by Zeiss (1 mentions) Pcs 100 011, by American Type Culture Collection (1 mentions) Cc 2545, by Lonza (1 mentions) C 12203, by PromoCell (1 mentions) Fetal bovine serum (fbs), by Omega Scientific (1 mentions) C 22111, by PromoCell (1 mentions) Hdmec, by Lonza (1 mentions) Egm 2, by PromoCell (1 mentions) Hdmec, by PromoCell (1 mentions)

4 protocols using c 12212

Rickettsia Infection Protocol in Vero and HDMEC Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols

Vero76 cells (Vero, ATCC CCl-81) were maintained in RPMI 1640 supplemented with 5% fetal bovine serum and were grown at 37°C. Vero cell-Rickettsia infections were performed using M199 media supplemented with 2% FBS and grown at 34°C.
Primary human dermal microvascular endothelial cells from adult or juvenile donors were purchased from PromoCell (C-12212) (Heidelberg, Germany) and maintained in Endothelial Growth Media MV 2 (PromoCell C-22121). The cells were grown at 37°C, 5% CO₂ in a humidified incubator and were maintained, stored, and split according to manufacturer’s instructions. HDMEC-Rickettsia infections were performed in the same media but at 34°C.

Fitzsimmons L., Bublitz D., Clark T, & Hackstadt T. (2024). Rickettsia rickettsii virulence determinants RARP2 and RapL mitigate IFN-β signaling in primary human dermal microvascular endothelial cells. mBio, 15(4), e03450-23.

+ Open protocol

+ Expand

Endothelial Cell Culture and Characterization

Check if the same lab product or an alternative is used in the 5 most similar protocols

Human ECs were purchased from different vendors to capture a broad spectrum of sampling. Specifically, we obtained HUVECs (C2519A; Lonza; C-12203; PromoCell), HSaVECs (HSVEC/A; VEC-Technologies; cAP-0019; Angio-Proteomie), HAECs (PCS-100-011; ATCC; 6100; ScienCell), HIAECs (CC-2545; Lonza; cAP-0020; Angio-Proteomie), HBMVECs (ACBRI 376 V; Cell-Systems; cAP-0002; Angio-Proteomie), HUMVECs (C-12295; PromoCell; 7000; ScienCell), HLMVECs (3000; ScienCell; C-12281; PromoCell), HAMVECs (7200; ScienCell), and HDMECs (C-12212; PromoCell; 2000; ScienCell). All types of ECs were cultured in EGM-2 endothelial growth medium (CC-3162; Lonza) supplemented with 5% FBS (Omega Scientific) and were maintained at 37°C with 5% CO₂. Similar EC passages 4–6 were used for final experiments. Human dermal fibroblasts were a gift from W. Lowry (University of California, Los Angeles, Los Angeles, CA), expanded in DMEM supplemented with 10% FBS, and cultured in EMG-2 medium over 48 h before RNA expression experiments. Brightfield images were acquired by using an Axiovert 200M inverted microscope (Zeiss).

Hilfenhaus G., Nguyen D.P., Freshman J., Prajapati D., Ma F., Song D., Ziyad S., Cuadrado M., Pellegrini M., Bustelo X.R, & Iruela-Arispe M.L. (2018). Vav3-induced cytoskeletal dynamics contribute to heterotypic properties of endothelial barriers. The Journal of Cell Biology, 217(8), 2813-2830.

+ Open protocol

+ Expand

Cultivation of Human Dermal Microvascular Endothelial Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols

Human dermal microvascular endothelial cells (HDMECs) were obtained commercially from Promocell (C-12212). Cell passage was designated as P1 upon arrival from the manufacturer. HDMECs were cultured in tissue culture polystyrene flasks coated with 0.1% gelatin (30 min at 37°C) with Endothelial Growth Medium-2 (EGM2; Lonza CC-3162 or Promocell C-22111) for expansion and in EV-depleted EGM2 for experiments. EGM2 was depleted of EVs as previously described [19 ]. Briefly, fetal calf serum (FCS) or fetal bovine serum (FBS) provided in the EGM2 kits were centrifuged at 100,000 x g for 16 h, sterile filtered with a 0.2 μm syringe filter, and combined with other components of the kit to make EV-depleted EGM2.

Patel D.B., Luthers C.R., Lerman M.J., Fisher J.P, & Jay S.M. (2018). Enhanced extracellular vesicle production and ethanol-mediated vascularization bioactivity via a 3D-printed scaffold-perfusion bioreactor system. Acta biomaterialia, 95, 236-244.

+ Open protocol

+ Expand

Culturing Human Endothelial Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols

Human aortic EC (HAoEC; C-12271), HUVEC; C-12206, and human dermal microvascular EC (HDMEC; C-12212) were purchased from PromoCell (Heidelberg, Germany). HUVEC were cultured in EGM-2 (Lot No. 406M001), and HAoEC and HDMEC were cultured in MV-2 (Lot No. 411M081), and all cell lines were supplemented and mixed with 1% penicillin/streptomycin. All cell lines were cultured at 37 °C in a humidified incubator containing 5% CO₂.

Lee J.H., Parveen A., Do M.H., Kang M.C., Yumnam S, & Kim S.Y. (2020). Molecular mechanisms of methylglyoxal-induced aortic endothelial dysfunction in human vascular endothelial cells. Cell Death & Disease, 11(5), 403.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!