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Jem 1400 plus microscope

Manufactured by JEOL
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Sourced in Japan

The JEM-1400 Plus is a transmission electron microscope (TEM) designed for high-resolution imaging and analysis of a wide range of materials. It features a LaB6 electron source and a 120 kV accelerating voltage, providing excellent image quality and analytical capabilities. The JEM-1400 Plus is equipped with advanced features for enhanced performance and versatility, making it a reliable tool for researchers and scientists across various fields.

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Lab products found in correlation

Glow discharged formvar carbon coated copper grid, by Electron Microscopy Sciences (6 mentions) Glow discharged holey carbon grid, by Quantifoil (3 mentions) Fcf400 cu 50, by Electron Microscopy Sciences (3 mentions) Matataki cmos camera, by JEOL (2 mentions) Tecnai f20 microscope, by Thermo Fisher Scientific (2 mentions) Ruby ccd camera, by JEOL (2 mentions) Jem 1011 microscope, by JEOL (1 mentions) Dynapro plate reader 2, by Wyatt Technology (1 mentions) J 725, by Jasco (1 mentions) Jnm ex400, by JEOL (1 mentions) V 560, by Jasco (1 mentions) Jes x320, by JEOL (1 mentions) Spectra300 stem, by Thermo Fisher Scientific (1 mentions) Whatman, by Cytiva (1 mentions) Geminisem 560, by Zeiss (1 mentions) D8 advance diffractometer, by Bruker (1 mentions) Alpha ftir spectrometer, by Bruker (1 mentions) Supra 55 instrument, by Zeiss (1 mentions) Spinsr10 confocal microscope, by Olympus (1 mentions) Poly bed 812 embedding kit, by Polysciences (1 mentions)

Spelling variants of this product

JEM-1400 (1333 citations) JEM-1400 Plus transmission electron microscope (168 citations) JEM-1400 microscope (118 citations) JEM-1400 Plus electron microscope (88 citations) JEOL 1400 Plus (76 citations) JEOL 1400 microscope (17 citations) JEM-1400(PLUS) 120 kV transmission electron microscope (4 citations) JEM-1400 Plus TEM transmission electron microscope (2 citations)

45 protocols using jem 1400 plus microscope

1

Structural characterization of nanocrystals

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TEM images of nanocrystals
were acquired on a JEOL JEM-1011 microscope equipped with a thermionic
gun at an accelerating voltage of 100 kV and on a JEOL JEM-1400Plus
microscope working at 120 kV. Selected area electron diffraction (SAED)
patterns were acquired on a JEOL JEM-1400Plus microscope. The samples
were prepared by depositing 3 μL of a diluted nanocrystal suspension
in trichloroethylene (TCE) onto 200-mesh carbon-coated copper grids
and letting it dry slowly. The indexation of SAED data was performed
with the help of the CaRIne Crystallography software suite via a simulation
of the reciprocal atomic lattice. HRSEM images were acquired on a
JEOL JSM-7500FA scanning electron microscope (SEM). Optical images
were acquired on a ZETA-20 true color 3D optical profiler.
Toso S., Baranov D., Altamura D., Scattarella F., Dahl J., Wang X., Marras S., Alivisatos A.P., Singer A., Giannini C, & Manna L. (2021). Multilayer Diffraction Reveals That Colloidal Superlattices Approach the Structural Perfection of Single Crystals. ACS Nano, 15(4), 6243-6256.
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2

Particle Size Characterization by TEM, NTA, and DLS

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The particle samples were examined by transmission electron microscopy (TEM) using a JEOL JEM-1400 PLUS microscope at 100 kV (JEOL Ltd., Japan). Samples were prepared for TEM imaging by pipetting 10 μL of the particle suspension onto a pioloform-coated single slot grid (Ted Pella, Cu, Pelco Slot Grids, USA), after which the water was allowed to evaporate, leaving the particles on the grid. Micrographs were recorded with a JEOL Matataki CMOS camera using TEM Centre for JEM1400 Plus software. The particle size distribution was later analysed from the images using ImageJ image processing and analysis software.36 Nanoparticle tracking analysis (NTA) was performed using a NanoSight LM10 instrument (Nanosight, England) equipped with a 405 nm laser. A sample volume of 400 μL was used and 60 s videos were recorded and analysed using NTA 2.3 software (Nanosight, England). Size data are reported as the mean (by concentration) and mode. Data were collected from four independently mixed samples per treatment.
Dynamic light scattering (DLS) measurements were conducted on a DynaPro Platereader-II, Wyatt Technology Corp. USA. The sample volume was 100 μL and each sample was recorded 10 consecutive times with an acquisition time of 10 s at 25 °C. Data were analysed using analysis software Dynamics V7.
Ekvall M.T., Lundqvist M., Kelpsiene E., Šileikis E., Gunnarsson S.B, & Cedervall T. (2018). Nanoplastics formed during the mechanical breakdown of daily-use polystyrene products. Nanoscale Advances, 1(3), 1055-1061.
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3

Liposome Morphology Analysis by TEM

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The uncoated/coated liposome shape and surface morphology was examined by TEM. Samples were prepared by placing a 200-mesh copper grid coated with carbon on a drop of the liposome preparation and waiting for 5 min to allow for sample deposition on the grid. Negatively stained TEM samples were prepared by dipping the liposomes-loaded grid on a 2% (w/v) uranyl acetate solution for another 5 min. Excess sample and reagent were removed from the grid using a filter paper, then the grids were left to dry completely. Micrographs were recorded using a Jeol JEM-1400 Plus microscope (JEOL Ltd.; Tokyo, Japan) working at an accelerating voltage of 100 kV.
Alghurabi H., Tagami T., Ogawa K, & Ozeki T. (2022). Preparation, Characterization and In Vitro Evaluation of Eudragit S100-Coated Bile Salt-Containing Liposomes for Oral Colonic Delivery of Budesonide. Polymers, 14(13), 2693.
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4

Transmission Electron Microscopy of Nanocrystals

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Transmission
electron microscopy (TEM) analyses were carried out on a JEOL JEM-1400Plus
microscope with a thermionic gun (LaB6 crystal) working
at an acceleration voltage of 120 kV. The samples for TEM measurement
were prepared by dropping dilute NC hexane solutions onto carbon film-coated
200 mesh copper grids.
Liu Y., Zaffalon M.L., Zito J., Cova F., Moro F., Fanciulli M., Zhu D., Toso S., Xia Z., Infante I., De Trizio L., Brovelli S, & Manna L. (2022). Cu+ → Mn2+ Energy Transfer in Cu, Mn Coalloyed Cs3ZnCl5 Colloidal Nanocrystals. Chemistry of Materials, 34(19), 8603-8612.
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5

Thermal Analysis of Viologen-Modified Glutamide Derivatives

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All chemicals were of reagent grade and were purchased from chemical suppliers (Tokyo Chemical Industry Co., Ltd., FUJIFILM Wako Pure Chemical Corp., and Sigma-Aldrich, Inc.). The NMR spectra, UV-visible (UV-vis) spectra, circular dichroism (CD) spectra, and electron paramagnetic resonance (EPR) spectra were measured using JNM-EX400 (JEOL, Tokyo, Japan), V-560 (JASCO, Tokyo, Japan), J-725 (JASCO, Tokyo, Japan), and JES-X320 (JEOL, Tokyo, Japan) spectrometers, respectively. Transmission electron microscopy (TEM) was conducted using a JEM-1400 Plus microscope (JEOL, Tokyo, Japan). Differential scanning calorimetry (DSC) thermograms (Fig. S1†) were obtained using a DSC 6200 differential scanning calorimeter (Seiko Instruments Inc., Chiba, Japan). An aqueous solution of viologen-modified glutamide (G) derivatives, G-V2+, (20 mM, 50 μL; Fig. 2a), was sealed in 70 μL silver pans and scanned between 5 and 90 °C at a heating and cooling rate of 2 °C min−1 under a N2 atmosphere (50 mL min−1).
Kuwahara Y., Ito M., Iwamoto T., Takafuji M., Ihara H., Ryu N, & Mani T. (2022). Chemical redox-induced chiroptical switching of supramolecular assemblies of viologens. RSC Advances, 12(4), 2019-2025.
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6

Cryo-EM and Negative Staining Imaging

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Negative-staining was achieved by adsorbing ~5 μL of sample on a glow-discharged formvar/carbon coated copper grid (Electron Microscopy Sciences) and staining with 2% uranyl formate. Imaging was performed on a JEOL JEM-1400 Plus microscope operated at 80kV. Samples for cryo-EM were first concentrated using centrifugal filters with 30-kD nominal molecular weight limit (EMD Millipore) to remove iodixanol and achieve ~50 nM DNA-ring concentration. The concentrated samples were then adsorbed on glow-discharged holey carbon grids (Quantifoil MicroTools GmbH), which were subsequently washed with buffer solution with 10% reduced salt concentration, blotted, and flash frozen in liquid ethane using FEI Vitrobot. Imaging was performed on an FEI Tecnai-F20 microscope operated at 200kV.
Yang Y., Wang J., Shigematsu H., Xu W., Shih W.M., Rothman J.E, & Lin C. (2016). Self-assembly of size-controlled liposomes on DNA nanotemplates. Nature chemistry, 8(5), 476-483.
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7

Negative Staining of DNA Nanostructures

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To prepare negatively stained DNA nanostructures or DNA-origami-organized liposomes with or without proteins, a drop of sample (5 μL) was deposited on a glow discharged formvar/carbon coated copper grid and incubated for 1–3 min at RT. Fluid was then removed by blotting with a filter paper. The grid was immediately stained for 3 min with 2% (w/v) uranyl formate. Grids were examined using a JEOL JEM-1400 Plus microscope equipped with a LaB6 filament (acceleration voltage: 80 kV). Images were acquired by an Advanced Microscopy Technologies bottom-mount 4k×3k CCD camera.
Bian X., Zhang Z., Xiong Q., Camilli P.D, & Lin C. (2019). A programmable DNA-origami platform for studying lipid transfer between bilayers. Nature chemical biology, 15(8), 830-837.
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8

Negative Staining and Electron Microscopy

Cited 1 time
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A drop (∼5
μL) of sample was deposited on a glow-discharged carbon-coated
copper grid and allowed to adsorb for 1–2 min before blotted
away. Negative-stain was achieved by adding 2% uranyl formate solution
to the grid and incubating for 1–3 min. After blotting away
the stain, the grid was air-dried and imaged using a JEOL JEM-1400
plus microscope operated at 80 kV. Images were acquired by a 4k ×
3k CCD camera (Advanced Microscopy Technologies) and analyzed by ImageJ
(National Institutes of Health) for tubule abundance and dimensions,
and EasyWorm40 (link) for persistence lengths.
Grome M.W., Zhang Z, & Lin C. (2019). Stiffness and Membrane Anchor Density Modulate DNA-Nanospring-Induced Vesicle Tubulation. ACS Applied Materials & Interfaces, 11(26), 22987-22992.
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9

Negative Stain Transmission Electron Microscopy

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5 μL of the sample was deposited on a glow-discharged formvar/carbon-coated copper grid (Electron Microscopy Sciences, catalog number FCF400-Cu-50), incubated for 1 min and blotted away. The grid was washed briefly with 2% (w/v) uranyl formate (Electron Microscopy Sciences, catalog number 22450) and stained for 1 min with the same uranyl formate buffer. Images were acquired using a JEOL JEM-1400 Plus microscope with an acceleration voltage of 80 kV and a bottom-mount 4k × 3k charge-coupled device camera (Advanced Microscopy Technologies, AMT).
Peng L., Renauer P.A., Ökten A., Fang Z., Park J.J., Zhou X., Lin Q., Dong M.B., Filler R., Xiong Q., Clark P., Lin C., Wilen C.B, & Chen S. (2022). Variant-specific vaccination induces systems immune responses and potent in vivo protection against SARS-CoV-2. Cell Reports Medicine, 3(5), 100634.
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10

Electron Microscopy of Nanowires and Cells

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Electron microscopy science grids (mesh size 400) were plasma cleaned for 30 s in PDC-001-HP Harrick Plasma cleaner. About 5 µl of sample (nanowires or cell culture) was dropped onto the carbon face of the grid and left to stand for 10 min. Excess solution was blotted. Samples were placed face down on 50 µL drops of negative stain (1% phosphotungstic acid, pH 6) for 30 s; the remaining solution was removed by blotting, then repeated. Grids were air-dried and stored in a sealed case until imaging using a JEM-1400Plus microscope operating at 80 kV (JEOL).
Portela P.C., Shipps C.C., Shen C., Srikanth V., Salgueiro C.A, & Malvankar N.S. (2024). Widespread extracellular electron transfer pathways for charging microbial cytochrome OmcS nanowires via periplasmic cytochromes PpcABCDE. Nature Communications, 15, 2434.
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