Lutalyse

Manufactured by Zoetis

Sourced in United States

Lutalyse is a veterinary product manufactured by Zoetis. It is a synthetic prostaglandin analog that assists in the regulation of the estrous cycle in livestock animals. The core function of Lutalyse is to synchronize and control the timing of estrus and ovulation in cattle, swine, and horses.

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Lab products found in correlation

Factrel, by Zoetis (12 mentions) Eazi breed cidr, by Zoetis (6 mentions) Xylocaine, by Fresenius (3 mentions) Eazi breed, by Zoetis (2 mentions) Lps escherichia coli o55 b5, by Merck Group (1 mentions) Dinoprost tromethamine, by Zoetis (1 mentions) Gonadorelin hydrochloride, by Zoetis (1 mentions) Estrumate, by Merck Group (1 mentions) Dnase 1, by Worthington (1 mentions) Prosound 2, by Aloka (1 mentions) Gonadiol, by Zoetis (1 mentions) Folltropin, by Vetoquinol (1 mentions) Prostaglandin f2α, by Zoetis (1 mentions) Wnt3a, by R&D Systems (1 mentions) Ssd 500v, by Aloka (1 mentions) Lutalyse highcon, by Zoetis (1 mentions) Eazi breed cidr cattle insert, by Zoetis (1 mentions) Pgf2α, by Zoetis (1 mentions)

37 protocols using lutalyse

Bovine Corpora Lutea: Synchronized and Pooled

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Female cattle (n = 15) of composite breeding were synchronized using two intramuscular injections of prostaglandin F2α (25 mg; Lutalyse®, Zoetis Inc., Kalamazoo, MI) 11 days apart. At day 3 or day 10 after ovulation, 3-5 cows were subjected to a bilateral ovariectomy through a right flank approach under local anesthesia, as previously detailed (29, 30) .
Luteal tissue was prepared for microscopy and remaining tissue was frozen using liquid N2. Day 10 corpora lutea weighed significantly more than day 3 corpora lutea (4.7 ± 0.46 vs. 2.8 ± 0.65 g, respectively). However, in all other measures examined in the course of this study there were no significant differences. Therefore, data from both day 3 and day 10 were pooled to provide additional statistical power and narrow the confidence intervals associated with progesterone secreting bovine corpora lutea. All animal procedures were completed under an IACUC-approved protocol and performed at the University of Nebraska-Lincoln, Animal Sciences Department.

Talbott H., Plewes M.R., Krause C., Hou X., Zhang P., Rizzo W.B., Wood J.R., Cupp A.S., & Davis J.S. (2020). Composition of the Lipid Droplets of the Bovine Corpus Luteum.

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Somatotropin Effects on Follicular Dynamics

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The study was performed with non-lactating Jersey dairy cows (n=21), kept in pasture. The cows had body condition score (BCS) between 2.5 and 3.5 (on a scale of 1 to 5) and were randomly (by BCS) assigned to two treatments: control (n=10) and somatotropin (rbST, n=11). The rbST group received one injection of somatotropin (500 mg/cow, s.c., Lactotropin®, Elanco, SP, Brazil) 60 days before the beginning of synchronization protocol (Day -60). On day 0, all cows received an intravaginal progesterone (P4)-releasing device (1.9 g, CIDR®, Zoetis, NJ, USA) and a GnRH injection (100 mg, IM, Factrel®, Zoetis). On Day 8, at the moment of P4 device withdrawal, all cows received an injection of PGF2α (25 mg, i.m., Lutalyse®, Zoetis). Transrectal ultrasonography (7.5 MHz linear array probe, Aquila pro, Esaote, São Paulo, SP, Brazil) was performed on days -60, -53, -46, -14, -7, 0 and 8 for antral follicle count. All follicles detected by ultrasonography were counted and classified according to its diameter in three categories: Class 1 (<6 mm), Class 2 (6-9 mm) and Class 3 (>9 mm). On day 8 of the protocol, the diameter of the largest follicle was measured through ultrasonography.

Rincón J.A., Mion B., Acosta D.A., Gasperin B.G., Rovani M.T., Pegoraro L.M., Corrêa M.N, & Schneider A. (2019). Effect of recombinant bovine somatotropin (rbST) treatment on follicular population and development in non-lactating dairy cows. Animal Reproduction, 16(4), 914-922.

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Sow Farrowing Crate Management Protocol

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Each sow was housed in an individual farrowing crate, located in the center of a farrowing pen; the flooring was of either woven metal or perforated plastic. Flooring type was balanced across treatments. Crate dimensions were 0.6 m wide by 2.0 m long, giving a floor space within the crate of 1.2 m²; pen dimensions were 1.5 m wide by 2.1 m long, giving a total pen floor space of 3.2 m². Crates were equipped with a sow-operated feed dispenser attached to a feed trough and a nipple-type water drinker for the sow. An infrared heat lamp was suspended in the center of the floor area over an insulated rubber mat located on one side of the farrowing pen. Farrowing room air temperature was maintained using heaters, evaporative cooling cells, and fan ventilation as needed; room thermostats were set at 22.5 °C throughout the study period.
Management in the farrowing facility was according to farm protocols. Sows that had not farrowed by d 116 of gestation were induced to farrow on the following d using Lutalyse (1 injection of 1 mL given at 0600 h; Zoetis; Parsippany, NJ); the identity of each sow induced and date of induction were recorded. The farrowing process was monitored continuously by the investigators; if the interval between the births of piglets exceeded 60 min, the investigator checked the birth canal for obstructions, and assisted the farrowing process as needed.

Vande Pol K.D., Tolosa A.F., Bautista R.O., Willard N.C., Gates R.S., Shull C.M., Brown C.B., Alencar S.A., Lents C.A, & Ellis M. (2021). Effects of drying and providing supplemental oxygen to piglets at birth on rectal temperature over the first 24 h after birth. Translational Animal Science, 5(3), txab095.

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Bovine Embryo Collection Protocol

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Bovine peri-implantation embryos were collected 12, 14, 16 18 days after artificial insemination. Embryos were recovered by standard non-surgical flush with lactated ringer solution supplemented with 1% fetal bovine serum and washed with PBS before processing for single cell isolation. All cows were treated with prostaglandin (Lutalyse, Zoetis) after flushing.

Scatolin G.N., Ming H., Wang Y., Iyyappan R., Gutierrez-Castillo E., Zhu L., Sagheer M., Song C., Bondioli K, & Jiang Z. (2024). Single-cell transcriptional landscapes of bovine peri-implantation development. iScience, 27(4), 109605.

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Synchronizing Ovulation in Dairy Cows with Autoclaved or Chemically Disinfected Progesterone Implants

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Cows were randomly assigned to 1 of 2 treatment groups using a completely randomized design of treatments. At the beginning of the FTAI protocol (d -10), the cows received an autoclaved (AUT; n = 177) or chemically disinfected (CHEM; n = 172) 8-d used intravaginal P4 implant (CIDR, Zoetis, São Paulo, Brazil; 1.9 g of P4) that remained for 8 d. Immediately after P4 insertion, cows were treated with EB (Gonadiol, MSD Saúde Animal, São Paulo, Brazil; 2.0 mg of EB i.m.) and GnRH (Fertagyl, MSD Saúde Animal, 100 µg of gonadorelin i.m.). At 7 (d -3) and 8 (d -2) d after implant insertion, PGF 2α (Lutalyse, Zoetis, 25 mg of dinoprost tromethamine i.m.) was administered, and on d -2, after withdrawal of the P4 implant, cows received EC (ECP, Zoetis; 1.0 mg of EC i.m.) to synchronize ovulation. Fixed-time AI was performed at d 0, 48 h after EC administration, and cows were bred with conventional frozen/thawed semen from Holstein sires (Figure 1).
After treatments on d -2, a subset of 115 cows from farm A received a heat detection device (Estrotect, IVP Brasil, São Paulo, Brazil), which remained until d 0, the time of AI. Cows that had greater than half of the patch coating removed were classified as exhibiting standing estrus.

Melo L.F., Monteiro PLJ J.r., Nascimento A.B., Drum J.N., Spies C., Prata A.B., Wiltbank M.C, & Sartori R. (2018). Follicular dynamics, circulating progesterone, and fertility in Holstein cows synchronized with reused intravaginal progesterone implants that were sanitized by autoclave or chemical disinfection. Journal of dairy science, 101(4).

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Synchronizing Ovulation in Cattle

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A 5 d Co-Synch + Control Internal Drug Release (CIDR) protocol was initiated at 77 ± 5 DPP to synchronize ovulation. At protocol initiation, all cows received a CIDR (CIDR Zoetis, Florham Park, NJ) paralleled with the administration of 100μg of GnRH (Cystorelin, Merial Animal Health, Duluth, GA). Five days after protocol initiation, the CIDR was removed and 2 separate 25-mg injections of PGF_2α (Lutalyse, Zoetis) were simultaneously administered. At 72 h post PGF_2α injection, all cows were time-bred using artificial insemination (TAI), paralleled with the administration of 100μg of GnRH (Cystorelin, Merial Animal Health). Cows were bred by 1 of 2 technicians. Trans-rectal ultrasonography was used to measure any ovarian follicle with diameters ≥ 7 mm at TAI and again at 48 h post-TAI to confirm which follicle underwent ovulation. All cows were exposed to natural mating for a total of a 60 d breeding season. Pregnancy diagnosis was performed at 39 d after TAI to determine TAI pregnancy rates. Final pregnancy diagnosis was conducted 35 d after termination of the breeding season.

Taylor E.G., Lemenager R.P, & Stewart K.R. (2017). Using corn gluten feed in post-partum diets of young beef cows to optimize reproductive performance. Translational Animal Science, 1(3), 296-303.

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Reproductive Management of Primiparous Cows

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For experiment 1, weekly cohorts of primiparous cows were enrolled in the study at 60 ± 3 DIM (study d 0). Cows (n = 1,358) were assigned randomly based on ear tag identification number to 2 treatments: (1) treatment with 100 μg of GnRH (Factrel, Zoetis Inc., Florham Park, NJ; Gpresynch), or (2) no treatment (control) at study d 0. At study d 7, all cows were presynchronized with two 25-mg injections of PGF 2α (dinoprost tromethamine; 5 mL of Lutalyse, Zoetis Inc.) injections given 14 d apart. Estrus detection was performed once daily based on tail paint removal. Cows detected in estrus were eligible to be inseminated after 53 DIM. Once inseminated, cows received no further injections of the synchronization protocol. Cows not inseminated by study d 35 were enrolled in the Cosynch-72 protocol for TAI (Figure 1).

Voelz B.E., Rocha L., Scortegagna F., Stevenson J.S, & Mendonça L.G.D. (2016). Treatment of lactating dairy cows with gonadotropin-releasing hormone before first insemination during summer heat stress. Journal of dairy science, 99(9).

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Immune Response of Heifers to LPS

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All procedures were approved by the New Mexico State University Institutional Animal Care and Use Committee (2017–030). Fourteen crossbred heifers (BW average = 416 kg) were housed and maintained at New Mexico State University (Las Cruces, NM).
Heifers were stratified by BW and assigned to vehicle-treated control (CON, n = 7) or LPS (n = 7) treatment groups. LPS (Escherichia coli O55:B5; Sigma–Aldrich, Inc., Milwaukee, WI) was prepared by dissolving 1 mg in 5 mL of sterile saline, then subcutaneously injected at a rate of 2.0 μg/kg of BW. Each animal received approximately 4 mL of solution on days 2, 5, and 8. Control animals were injected with sterile saline. Baseline rectal temperatures were taken 1 d before the start of the experiment to preclude any potential febrile response prior to administering treatment.
Heifers were synchronized to estrus using the Select Synch plus controlled internal drug release (CIDR) protocol. On day 0, a GnRH (2 mL, i.m., Factrel, Zoetis Inc. US) injection was administered, and a CIDR (Eazi-Breed, Zoetis Inc., Kalamazoo, MI) device was inserted. On day 7, the CIDR was removed, and heifers received PGF2á (Lutalyse, 25 mg, i.m., Zoetis Inc. US).
Rectal temperatures were taken just prior to treatment (30-Second Digital Thermometer, Target Corporation, Minneapolis, MN) and at 2, 3, 4, 8, and 10 h after injection.

Ferranti E.M., Aloqaily B.H., Gifford C.A., Löest C.A., Wenzel J.C, & Hernandez Gifford J.A. (2018). Lipopolysaccharide modulation of ovarian hormonal profile. Translational Animal Science, 2(Suppl 1), S31-S34.

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Synchronizing Estrus for Embryo Transfer

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Recipient estrus synchronization was initiated by inserting an intravaginal progesterone device (1.38 g; Eazi-Breed CIDR; Zoetis) and intramuscular administration of gonadotropin (100 mcg; Factrel; Zoetis) on day 0 (sixteen days prior to transfer). On day 7, the CIDR was removed and intramuscular prostaglandin (25 mg; Lutalyse; Zoetis) was administered. Recipients were monitored for estrus, and a second intramuscular dose of gonadotropin (100 mcg; Factrel; Zoetis) was administered on day 9. Prior to transfer on day 16, recipient response to synchronization was confirmed via detection of an appropriate corpus luteum with transrectal ultrasonography. Prior to transfer, each recipient received a caudal epidural using 100 mg 2% lidocaine (Xylocaine; Fresenius). Embryos were transferred via non-surgical, transcervical technique, and the blastocyst was deposited into the uterine horn ipsilateral to the corpus luteum. Pregnancy was diagnosed on day 35 of embryonic development by transrectal ultrasonography (5.0 MHz linear probe; EVO Ibex, E.I. Medical Imaging).

Owen J.R., Hennig S.L., McNabb B.R., Lin J.C., Young A.E., Murray J.D., Ross P.J, & Van Eenennaam A.L. (2020). Harnessing endogenous repair mechanisms for targeted gene knock-in of bovine embryos. Scientific Reports, 10, 16031.

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Embryo Transfer and Hormone Treatments in Cows

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The study was approved by the Institutional Animal Care and Use Committee of Mississippi State University (11-023) and implemented at The Coastal Plain Branch Experiment Station of Mississippi State University in Newton, MS in the Spring of 2011. Lactating Angus crossbred cows were synchronized for estrous by receiving a CIDR (Eazi-Breed CIDR; Zoetis, Madison NJ) for 7 d. One d after removal, all cows (n = 62) received an injection of PGF2α (25 mg IM; Lutalyse; Zoetis). Cows were observed for estrus (d 0) four times per d (1 h at each time) during the 80 h post-PGF2α. Following manual evaluation of the CL via palpation per rectum, all cows exhibiting estrus with a CL received an embryo in the uterine horn ipsilateral to the CL on 7 d after estrus. At the time of transfer, cows were assigned to 1 of 4 treatments: no further treatment (Control, n = 16), a CIDR insert (CIDR, n = 16), an injection of hCG (1000 IU, IM; Sioux Biochemical, Inc, Sioux Center, IA; hCG, n = 15) or an injection of GnRH (100 μg, IM; Cystorelin; Merial, Duluth, GA; GnRH, n = 15).

Mason M.C., Copeland J., Cuadra E.J., Elsasser T.H., Jung Y, & Larson J. (2014). Dynamics of Progesterone, TNF-α, and a Metabolite of PGF2α in Blood Plasma of Beef Cows following Embryo Transfer. Veterinary Medicine International, 2014, 650272.

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