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Allprep kit

Manufactured by Qiagen
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Sourced in Germany, United States, United Kingdom, Netherlands

The AllPrep kit is a laboratory equipment product designed for the simultaneous purification of DNA, RNA, and proteins from a single biological sample. It utilizes a silica-based membrane technology to efficiently capture and purify these biomolecules. The kit provides a streamlined workflow for sample preparation, enabling researchers to obtain high-quality nucleic acids and proteins from a variety of sample types.

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Lab products found in correlation

Hiseq 4000, by Illumina (10 mentions) Bioanalyzer, by Agilent Technologies (9 mentions) Iscript cdna synthesis kit, by Bio-Rad (8 mentions) Turbo dnase, by Thermo Fisher Scientific (7 mentions) 2100 bioanalyzer, by Agilent Technologies (7 mentions) Agilent 2100 bioanalyzer, by Agilent Technologies (6 mentions) Tapestation, by Agilent Technologies (6 mentions) Rlt buffer, by Qiagen (6 mentions) Nanodrop 2000 spectrophotometer, by Thermo Fisher Scientific (5 mentions) Qiaamp dna blood maxi kit, by Qiagen (5 mentions) Nextseq 550, by Illumina (5 mentions) Hiseq 2000, by Illumina (4 mentions) Nanodrop nd 1000 spectrophotometer, by EQT (4 mentions) Qiaamp dna mini kit, by Qiagen (4 mentions) Novaseq 6000, by Illumina (4 mentions) Hiseq 2500, by Illumina (4 mentions) Rneasy mini kit, by Qiagen (4 mentions) High capacity cdna reverse transcription kit, by Thermo Fisher Scientific (4 mentions) Nanodrop nd 1000 spectrophotometer, by Thermo Fisher Scientific (3 mentions) Nanodrop, by Thermo Fisher Scientific (3 mentions)

Close spelling variants of this product

AllPrep DNA/RNA kit (336 citations) AllPrep DNA/RNA/miRNA kit (50 citations) AllPrep RNA/Protein Kit (45 citations) AllPrep DNA/RNA extraction kit (40 citations) AllPrep DNA Mini Kit (30 citations) AllPrep extraction kit (12 citations) AllPrep DNA FFPE Kit (10 citations) Allprep DNA/RNA purification kit (9 citations) Allprep DNA RNA mini prep kit (6 citations) AllPrep 96 PowerFecal DNA/RNA kit (5 citations)

307 protocols using allprep kit

1

Quantitative DNA Methylation Analysis of Lung Tumors

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RNA and DNA was purified from resected lung tumors using the All-Prep kit (Qiagen). Analysis of DNA methylation of repetitive elements was performed using EpiTYPER MassARRAY(25 (link),35 (link)). Genomic DNA was prepared from resected lung tumors using the All-Prep kit (Qiagen), and 1.0 μg of genomic DNA was bisulfite convereted using the EZ DNA methylation kit (Zymo Research). Regions of interest were amplified from bisulfite-treated DNA by PCR using primers designed in EpiDesigner (Agena Biosciences). Matrix-assisted laser desorption/ionization-time of flight (MALDI-ToF) mass spectrometry was performed to quantitate CpG methylation. Data was analyzed using EpiTYPER software 1.2 (Agena Biosciences) and visualized using R package ggplot2. For primers and target CpGs see supplemental table 4.
Koenig M.J., Agana B.A., Kaufman J.M., Sharpnack M.F., Wang W.Z., Weigel C., Navarro F.C., Amann J.M., Cacciato N., Arasada R.R., Gerstein M.B., Wysocki V.H., Oakes C, & Carbone D.P. (2021). STK11/LKB1 loss of function is associated with global DNA hypomethylation and S-adenosyl-methionine depletion in human lung adenocarcinoma. Cancer research, 81(16), 4194-4204.
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2

Investigating Macrophage Polarization in p110γ Knockout Mice

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Freshly isolated mouse bone marrow cells from 9 WT and 9 p110γ−/− mice were pooled into 3 replicates sets of WT or p110g−/− cells and differentiated into macrophages for six days in RPMI + 20% FBS+ 1%Pen/Strep+ 50 ng/ml M-CSF. Each replicate set of macrophages was then treated with mCSF, IL-4 or IFNg/LPS. Macrophages were removed from dishes, and RNA was harvested using Qiagen Allprep kit. In addition, RNA was harvested from day 14 (500mm3) LLC tumors or purified CD11b+Gr1-F480+ TAMs from WT (C57BL/6) and p110γ−/− null mice. RNA was harvested using Qiagen Allprep kit. RNA libraries prepared from 1 μg RNA per sample were prepared for sequencing using standard Illumina protocols. RNA sequencing was performed by the University of California, San Diego Institute for Genomic Medicine. mRNA profiles were generated by single read deep sequencing, in triplicate, using Illumina HiSeq2000.
Kaneda M.M., Messer K.S., Ralainirina N., Li H., Leem C., Gorjestani S., Woo G., Nguyen A.V., Figueiredo C.C., Foubert P., Schmid M.C., Pink M., Winkler D.G., Rausch M., Palombella V.J., Kutok J., McGovern K., Frazer K.A., Wu X., Karin M., Sasik R., Cohen E.E, & Varner J.A. (2016). PI3Kγ is a molecular switch that controls immune suppression. Nature, 539(7629), 437-442.
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3

Purification of Retinal and Choroidal Tissue RNA

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Tubes containing frozen neural retina or RPE-choroid tissue were placed in a dry ice/ethanol slurry and ground to a fine powder while still frozen. Upon transferring to ice, RLT lysis buffer from the Qiagen AllPrep kit was added to the sample and, following a second grinding, was further homogenized by passage through a QIAshredder column (Qiagen). DNA, RNA, and protein were purified from the flow-through using an AllPrep kit (Qiagen) per the manufacturer’s protocol. A second DNase I treatment was performed on all RNA samples. RNA integrity was measured using the Agilent Bioanalyzer RNA 6000 Pico chip assay. RNA concentration was determined using a nanodrop spectrophotometer. Additional methods for isolating RNA from isolated RPE and choroid are described in the SI Appendix.
Williams B.L., Seager N.A., Gardiner J.D., Pappas C.M., Cronin M.C., Amat di San Filippo C., Anstadt R.A., Liu J., Toso M.A., Nichols L., Parnell T.J., Eve J.R., Bartel P.L., Zouache M.A., Richards B.T, & Hageman G.S. (2021). Chromosome 10q26–driven age-related macular degeneration is associated with reduced levels of HTRA1 in human retinal pigment epithelium. Proceedings of the National Academy of Sciences of the United States of America, 118(30), e2103617118.
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4

RNA-seq analysis pipeline using Illumina HiSeq 4000

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RNAs were extracted with a Qiagene AllPrep kit on a QiaCube automated station. The samples were sequenced by JAX-GM Genome Technologies Core. RNA-seq libraries were prepared with a KAPA Stranded mRNA-Seq kit. The quantification of libraries was performed using a real-time qPCR. Sequencing was performed on an Illumina Hiseq 4000 platform, generating paired-end reads of 75 bp. Raw reads obtained from the sequencer were processed, including quality control steps to identify and remove low-quality samples. Reads with more than 50% low-quality bases (>Q30) overall were filtered out, and the remaining high-quality reads were then used for expression estimation.
Tang-Schomer M.D., Bookland M.J., Sargent J.E, & N. Jackvony T. (2023). Human Patient-Derived Brain Tumor Models to Recapitulate Ependymoma Tumor Vasculature. Bioengineering, 10(7), 840.
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5

Transcriptome Analysis Pipeline

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Nucleic acid extraction was performed using Qiagene AllPrep kit. PCR primers for DNA and cDNA are reported in Supplementary Table 2. PolyA-selected RNA libraries were prepared for RNA sequencing on the Illumina NextSeq platform using TruSeq chemistry according to the manufacturer's protocol. 150 bases long paired-end reads were assessed for quality and reads were mapped using CASAVA (Illumina). The generated fastq files were used as input for mapping using TopHat2 (ref). Cufflinks (http://cufflinks.cbcb.umd.edu/) was used to assemble and estimate the relative abundances of transcripts mapped with TopHat2 at both the gene and transcript level (FPKM). FPKM values were log2 transformed and Z-scored.
Jacoby E., Nguyen S.M., Fountaine T.J., Welp K., Gryder B., Qin H., Yang Y., Chien C.D., Seif A.E., Lei H., Song Y.K., Khan J., Lee D.W., Mackall C.L., Gardner R.A., Jensen M.C., Shern J.F, & Fry T.J. (2016). CD19 CAR immune pressure induces B-precursor acute lymphoblastic leukaemia lineage switch exposing inherent leukaemic plasticity. Nature Communications, 7, 12320.
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6

Intestinal Epithelial Cell Isolation

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Small intestines were opened longitudinally and washed in PBS. Villous epithelial cells were removed by gentle scraping with a coverslip. The intestine was cut into 1-mm pieces and incubated in 5 mM EDTA in HBSS for 35 min, and the epithelial cell layer was isolated by manual pipetting and collected for whole-intestine analysis or passed through a 70-μm filter to isolate crypts. Crypts were dissociated into single cells using 0.05% trypsin (HyClone), and GFP-positive cells that were confirmed as viable by DAPI were isolated using FACS performed on the FACSVAntage SE (BD Biosciences). Cells were immediately frozen in liquid nitrogen. DNA and RNA were isolated using the AllPrep Qiagen kit (Qiagen).
Sheaffer K.L., Kim R., Aoki R., Elliott E.N., Schug J., Burger L., Schübeler D, & Kaestner K.H. (2014). DNA methylation is required for the control of stem cell differentiation in the small intestine. Genes & Development, 28(6), 652-664.
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7

Isolation and Purification of Murine Pancreatic β Cells

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Pancreatic islets were isolated from 4-6 weeks and 16-20 months old C57BL6 male mice as previously described (Gupta et al., 2005 (link)). Isolated islets were dissociated into single cells, stained using an anti-insulin antibody (DAKO) and sorted using FACS to obtain a 98% pure β cell population. Cells were immediately frozen in liquid nitrogen. For downstream RNA analyses, flow cytometry sorting was performed on live β cells from isolated pancreatic islets of young and old MIP-GFP mice. DNA and RNA were isolated using the AllPrep Qiagen kit (Qiagen).
Avrahami D., Li C., Zhang J., Schug J., Avrahami R., Rao S., Stadler M.B., Burger L., Schübeler D., Glaser B, & Kaestner K.H. (2015). Aging-dependent demethylation of regulatory elements correlates with chromatin state and improved β-cell function. Cell metabolism, 22(4), 619-632.
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8

Genomic DNA Extraction from Tissues

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Genomic DNA was extracted from fresh frozen tissues. The samples were homogenized in lysis buffer consisting of 100 mM Tris-HCl (pH 8.5), 5 mM EDTA, 0.2% SDS, and 200 mM NaCl. Proteinase K was freshly added at a final concentration of 300 μg/mL. Samples were incubated overnight at 55°C to ensure that genomic DNA is completely dissociated from any DNA binding proteins. After digestion, genomic DNA was extracted using a QIAGEN's genomic DNA extraction kit (Germantown, CA), according to the manufacturer's instructions (AllPrep QIAGEN kit). DNA, quality, and quantity were assessed using a NanoDrop spectrophotometer and 0.8% agarose gel electrophoresis.
Ashktorab H., Shakoori A., Zarnogi S., Sun X., Varma S., Lee E., Shokrani B., Laiyemo A.O., Washington K, & Brim H. (2016). Reduced Representation Bisulfite Sequencing Determination of Distinctive DNA Hypermethylated Genes in the Progression to Colon Cancer in African Americans. Gastroenterology Research and Practice, 2016, 2102674.
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9

Reactivation of HIV from Latency

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Whole blood from five HIV+ patients at the Hospital Universitário Gaffrée e Guinle (Rio de Janeiro, Brazil) was collected for reactivation experiments. All individuals enrolled in the study voluntarily and provided written informed consent. Patients presented undetectable viral load (<50 copies/mL), more than 500 CD4+ T cells/mm3 and have been HAART-treated for at least 10 months (Table 1). PBMCs where isolated following a Ficoll-Paque Plus protocol (GE Healthcare, UK). CD4+ T cells were selected by magnetic beads as described above, and 106 cells were incubated in R10 containing 1 µM of the testing compounds (ING-B, prostratin or PMA). DMSO 1% was used as negative control. The antiretroviral EFV (10 µM) was added to all conditions to prevent viral spread. After 24 h, cells were collected for RNA isolation using the All Prep Qiagen kit (Qiagen), as instructed by the manufacturer.
Abreu C.M., Price S.L., Shirk E.N., Cunha R.D., Pianowski L.F., Clements J.E., Tanuri A, & Gama L. (2014). Dual Role of Novel Ingenol Derivatives from Euphorbia tirucalli in HIV Replication: Inhibition of De Novo Infection and Activation of Viral LTR. PLoS ONE, 9(5), e97257.
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10

Nucleic Acid Isolation Using Allprep

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RNA and DNA were isolated using the Allprep Qiagen Kit.
Kessler M., Hoffmann K., Fritsche K., Brinkmann V., Mollenkopf H.J., Thieck O., Teixeira da Costa A.R., Braicu E.I., Sehouli J., Mangler M., Berger H, & Meyer T.F. (2019). Chronic Chlamydia infection in human organoids increases stemness and promotes age-dependent CpG methylation. Nature Communications, 10, 1194.
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