C57bl 6j female mice

Female C57BL/6j mice, age 6–8 weeks, were bred inhouse at the Peter MacCallum Cancer Centre or obtained from Jackson Laboratories (L'Arbresle, France). All mice were maintained at the Animal Core Facilities at the Peter MacCallum Cancer Centre or University of Antwerp. All animal procedures were conducted in accordance with, and approval of, the Animal Ethics Committee of the Peter MacCallum Cancer Centre under registration numbers E498 and E582 or University of Antwerp under registration number 2016‐30. All mice were housed in filter‐top cages enriched with houses and nesting material. Mice were checked on a daily base to inspect health and wellbeing. Mice were given a 7‐day adaptation period upon arrival before being included in experiments to reduce stress levels.

Female BALB/c mice, 6-weeks of age, or female C57BL/6J mice, 2-months of age were purchased from Jackson Laboratory and maintained on standard mouse chow (Teklad, Harlan, catalog no. T7013M15, Indianapolis, Indiana, USA). The animal protocols used in this study were approved by the Animal Research Committee at UCLA and the methods were carried out in accordance with the approved guidelines.

Female C57BL/6 J mice (Jackson Labs, Bar Harbor, ME) weighing ca. 20 g were mated to males of the same strain for one to two hrs beginning at least two hrs into the light phase of a 12:12 light:dark schedule. When a copulatory plug was found, gestational day (GD) 0 was designated as the start of the mating session. Mated females were housed in clear, ventilated, polycarbonate cages with no more than three mice per cage, depending on the number of plugs detected in a mating session. Food (Isopro RMH 3000; Purina, St. Louis, MO) and water were freely available, housing and nesting material were provided, and the cob bedding was changed every other week. The vivarium was maintained at 21 ± 1 °C, 30–40% humidity. All mouse experiments followed NIH guidelines using methods approved by the IACUC of UNC at Chapel Hill. Zebrafish were obtained from Zebrafish International Resource Center. The AB strain was used in this study and fish were housed in automatic fish housing systems (Aquaneering, San Diego, CA) at 28.5 °C. All procedures using zebrafish were approved by the North Carolina Central University IACUC and conducted according to NIH and U.S. regulations.

Female C57BL6/J mice (Jackson Laboratories, Bar Harbor, ME; n = 40, 8/condition) from a larger study were randomly selected [17 (link)]. Phenotypic statistics for this subset were performed to validate the representation of the larger dataset. Animals were kept on a 12:12-h light–dark cycle and given access to normal rodent chow and water for the duration of the study. All animal protocols were approved by the University of Arkansas Institutional Animal Care and Use Committee, in accordance with the ethical standards (1964 Declaration of Helsinki).