The rabbit polyclonal antibody against RTTN was raised using recombinant RTTN-His (residues 1347–1591) and subjected to affinity purification. The following antibodies were used in this studies: antibodies against SAS-6 (residues 1–489, 1:500 dilution)13 (link), CPAP (residues 1070–1338, 1:1000 dilution)50 (link), CEP120 (residues 639–986, 1:1000 dilution)15 (link), CEP135 (residues 650–1140, 1:1000 dilution)9 (link), CEP295 (residues 2092–2430, 1:500 dilution)19 (link), centrobin (residues 443–626, 1:1000 dilution)13 (link), and centrin 2 (residues 1–173, 1:1000 dilution)13 (link). The other commercially available antibodies used in this study included anti-STIL (A302–442; 1:200 dilution), anti-hPOC5 (A303–341; 1:500 dilution) and anti-SPICE (A303–272; 1:500 dilution) (all from Bethyl); anti-hSAS-6 (H00163786; 1:100 dilution, monoclonal Ab), anti-centrin3 (H00001070-M01; 1:1000 dilution) and anti-CEP162 (PAB22408; 1:1000 dilution) (all from Abnova); anti-CP110 (12780-1-1p; 1:500 dilution; Proteintech); anti-CEP164 (NBP1-81445; 1:500 dilution; Novus Biologicals); anti-POC1B (PA5-24495; 1:500 dilution; Thermo Fisher); anti-acetylated tubulin (T6793; 1:1000 dilution) and anti-Flag (M2; F3165; 1:3000 dilution) (both from Sigma-Aldrich); and anti-GFP (632381; 1:3000 dilution; BD Bioscience).
Anti hpoc5
Manufactured by Fortis Life Sciences
Sourced in United States
Anti-hPOC5 is a laboratory reagent used for the detection and quantification of human POC5 protein. It is a highly specific antibody that binds to the POC5 protein, which is involved in centriole assembly and maintenance. The Anti-hPOC5 reagent can be used in various immunoassay techniques to measure POC5 levels in biological samples.
Automatically generated - may contain errors
Lab products found in correlation
Anti flag m2 f3165, by Merck Group (1 mentions)
Anti cep164 nbp1 81445, by Novus Biologicals (1 mentions)
Anti acetylated tubulin t6793, by Merck Group (1 mentions)
Anti gfp, by BD (1 mentions)
Anti γ tubulin gtu 88, by Merck Group (1 mentions)
Anti α tubulin dm1α, by Merck Group (1 mentions)
Anti numa, by Abcam (1 mentions)
2 protocols using anti hpoc5
1
Centrosome Protein Antibody Protocol
2
Antibody Characterization for RTTN and CEP120
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The antibodies against RTTN (1:500 dilution; rabbit polyclonal raised against RTTN, residues 1347-1591) [11 (link)] and CEP120 (1:1000 dilution; rabbit polyclonal raised against CEP120, residues 639-986) [23 (link)] were obtained as described previously. Other commercially available antibodies used in this study included anti-hPOC5 (Bethyl Laboratories, Inc., Montgomery, TX, USA; 1:500 dilution), anti-α-tubulin (DM1α, Sigma-Aldrich; 1:300 dilution), anti-γ-tubulin (GTU88, Sigma-Aldrich; 1:300 dilution), anti-CDK5RAP2 (Bethyl Laboratories, Inc.; 1:1000 dilution), anti-NuMA (Abcam; 1:500 dilution), anti-p150Glued (Transduction Laboratories; 1:300 dilution), and anti-pericentrin (BD Transduction Laboratories, Franklin Lakes, NJ, USA; 1:1000 dilution).
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