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9 protocols using cremophor rh60

1

Formulation and Evaluation of Enzalutamide Soft Capsules

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ENZ was purchased from MSN Labratories Private Ltd. (Telangana, India). The Xtandi® soft capsule, TPGS and MCT oil were supplied by Boryung Corporation (Seoul, Republic of Korea). Labrafac PG, Peceol, Labrasol, Capryol 90, Transcutol P, and Lauroglycol 90 were obtained from Gattefosse (St. Priest, France). Cremophor EL, Cremophor RH60, and Solutol HS15 were supplied by BASF (Ludwigshafen, Germany). Castor oil, coconut oil, olive oil, sesame oil, sunflower oil, corn oil, soybean oil, cotton seed oil, peanut oil, polysorbate 80, ammonium acetate, acetone, and acetonitrile were purchased from Daejung Co. Ltd. (Siheung, Republic of Korea). Sodium carboxymethyl cellulose was purchased from Duksan Pure Chemical (Ansan, Republic of Korea). All other chemicals used were of analytical grade.
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2

Itraconazole-Loaded Polymer Dispersions

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Itraconazole (Sigma-Aldrich), polyvinyl alcohol (PVA) Mw=72000, (Merck), Tween 80, Tween 20, Span 60 (Merck), Eumulgin CO40 (BASF), Cremophor RH 60 (BASF), Pluronic F127 (BASF), propylene glycol (PG) (Merck), benzyl benzoate (BB) (Merck), methanol (Merck), dichloromethane (DCM) (Merck), ethanol (Merck). Sabouraud 4% Dextrose Agar (SDA) (Merck), Sodium Dihydrogen Phosphate Dodecahydrate (Merck). Deionized distilled water was prepared using an in-house distillation assembly.
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3

Lipid Nanoparticle Formulation and Characterization

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All the phospholipids were provided by Avanti Polar Lipids and distributed by Sigma-Aldrich (Milan, Italy). Miglyol 812N was a gift from Sasol (Witten, Germany). Poly(lactide-co-glycolide) (PLGA) 75:25 (Resomer RG 752 H), trilaurin, ethyl acetate, benzyl alcohol, sodium taurodeoxycholate, Pluronic F68, Sepharose CL-4B, cholesterol and all other reagents were obtained from Sigma-Aldrich. Cremophor RH 60 (PEG-60 hydrogenated castor oil) was purchased from BASF (Ludwigshafen, Germany). All the solvents used were of analytical grade and purchased from Carlo Erba Reagenti (Milan, Italy). Solvent evaporation was carried out using a rotating evaporator (Heidolph Laborota 400, Heidolph Instruments, Schwabach, Germany) equipped with a vacuum pump (Diaphragm Vacuum Pump DC-4). TLC analyses were performed on silica gel aluminium plates (Macherey-Nagel, thick 25 µm, F254). HPLC analyses were carried out on a Waters instrument made up of 1525EF binary pump, W717 plus auto-sampler and 2996 PDA detector (Waters, Milan, Italy).
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4

Hyaluronic Acid Nanoparticles for Drug Delivery

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Hyaluronic acid sodium salt 1600 kDa (HA) was purchased from Farmalabor (Barletta, Italy), stearylamine (ST), cetyltrimethylammonium bromide (CTAB), ethyl acetate (EA), propylene glycol, benzyl alcohol, Pluronic®F68 (PL F68), polyvinyl alcohol 9000 (PVA 9000), and Kollidon®25 (PVP) were from Merck (Darmstadt, Germany), Epikuron®200 (phosphatidylcholine 92%) was from Cargill (Minneapolis, MN, USA), doxorubicin hydrochloride (DOXO) was purchased from APAC Pharmaceutical (Columbia, MD, USA), Cremophor®RH60 (PEG-60 hydrogenated castor oil) from BASF (Ludwigshafen am Rhein, Germany), taurocholic acid sodium salt (NaTC) and glycocholic acid sodium salt (NaGC) were from ICN Biomedicals (Aurora, OH, USA), and trilaurin and tristearin from Alfa-Aesar (Ward Hill, MA, USA). Deionized water was obtained by a MilliQ water purification system (Millipore, Bedford, MA, USA). Unless specified otherwise, all reagents were purchased from Merck.
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5

Formulation and Characterization of Doxorubicin Nanocarriers

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Trilaurin (TL), ethyl acetate (EA), benzyl alcohol (BenzOH), butyl lactate (BL), sodium taurocholate (NaTC), Pluronic® F68, 1,2 propanediol, CURC, cholesterol, chitosan and Sepharose® CL 4B, doxorubicin were purchased from Sigma Chemicals Co. (St. Louis, MO, USA). Epikuron®200 (lecithin-phosphatydilcoline 92%) was from Cargill (Minneapolis, MN, USA), Cremophor®RH 60 (PEG-60 hydrogenated castor oil) from BASF (Ludwigshafen, Germany). The plastic ware for cell cultures was obtained from Falcon (Becton Dickinson, Franklin Lakes, NJ, USA). The electrophoresis reagents were from Bio-Rad Laboratories (Hercules, CA, USA). The protein content of cell lysates was assessed with the BCA kit from Sigma Chemicals Co. Deionized water was obtained by a MilliQ system (Millipore, St. Louis, MO, USA). Unless specified otherwise, all reagents were purchased from Sigma Chemicals Co.
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6

Nanoparticle Formulation Development

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Trilaurin (TL), tristearin, myristic acid, glyceryl monostearate, ethyl acetate (EA), benzyl alcohol (BenzOH), triacetin (TA), butyl lactate (BL), polysorbate (20, 40, 80), sodium taurodeoxycholate (Na TdC), sodium taurocholate (Na TC), sodium glycocholate (Na GC), sodium cholate (Na C), Pluronic® F68, polyvinyl alcohol (PVA) 9000, PVA 14000, 1,2 propanediol, curcumin and Sepharose® CL 4B were purchased from Sigma (Dorset, UK). Trimyristin was from Alfa Aesar (Karlsruhe, Germany), glyceryl dibehenate was from Gattefossé (Weil am Rhein, Germany), Epikuron® 200 (lecithin-phosphatydil coline 92%) from Cargill (Minneapolis, MN, USA), and Cremophor® RH 60 (PEG-60 hydrogenated castor oil) from BASF (Ludwigshafen, Germany).
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7

Lipid-Based Nanoparticle Formulation

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Epikuron®200 was purchased from ACEF (Fiorenzuola d’Arda, Italy), benzyl alcohol, propylene glycol, stearoyl chloride, cholesterol, butyl lactate from Fluka (Buchs, Switzerland), Fluorescein isothiocyanate, CS low viscosity, Sepharose®CL 4B, CURC, potassium chloride, Poly(ethylene glycol) 300, 2,3-bis[2-methoxy-4-nitro-5-sulphophenyl]-2H-tetrazolium-5-carboxanilide, crystal violet solution from Sigma (Dorset, UK), Cremophor®RH60 from BASF (Ludwigshafen am Rhein, Germany), Taurocholic acid sodium salt from ICN Biomedicals (Solon, OH, USA), potassium phosphate monobasic, potassium phosphate dibasic from Merck (Darmstadt, Germany), methanol, trimethylamine, sodium chloride, acetic acid and ethanol from Carlo Erba (Val De Reuil, France). Deionized water was obtained by a MilliQ system (Millipore, Bedford, MO, USA).
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8

Antiangiogenic Pharmaceutical Formulation

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Propylene glycol was purchased from ACEF (Fiorenzuola d′Arda, Italy); benzyl alcohol, Sepharose®CL 4B, ethyl acetate, sodium sulfate, sodium dodecyl sulfate, phosphate buffer saline (PBS) tablets, trichloroacetic acid, M199 medium, heparin, MTT, VEGF-α, crystal violet, fetal calf serum, and antibiotics for cell cultures and sample buffer Laemmli were from Sigma-Aldrich (Dorset, UK); Epikuron®200 was from Cargill (Minneapolis, MN, USA); Cremophor®RH60 was from BASF (Ludwigshafen am Rhein, Germany); taurocholic acid sodium salt was from ICN Biomedicals (Aurora, OH, USA); potassium phosphate monobasic, potassium phosphate dibasic, and dioctyl sodium sulfosuccinate (AOT) were from Merck (Darmstadt, Germany), agar and trilaurin were from Alfa-Aesar (Ward Hill, MA, USA); hyaluronic acid sodium salt 1600 kDa (HA) was from Farmalabor (Barletta, Italy); Avastin® Roche (Basilea, Switzerland) was gently purchased from Molinette Central Hospital (Turin, Italy); sodium chloride (NaCl) and ethanol were from Carlo Erba (Val De Reuil, France). Boyden chamber filters were from Neuro Probe (BIOMAP snc, Milan, Italy) and Matrigel was from BD Biosciences. The retinal pigment epithelial ARPE-19 cell line (ATCC-CRL-2302) and the DMEM:F12 medium were purchased from ATCC® (Manassas, VA, USA).
Deionized water was obtained using a MilliQ water purification system (Millipore, Bedford, MA, USA).
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9

Formulation of Resveratrol-Loaded Nanoemulsion

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Resveratrol, castor oil, and polyethylene glycol (PEG) 1500 were purchased from Sigma-Aldrich (Germany). Cremophor® RH 60 was obtained from BASF (Germany). castor oil, methanol, ethanol, propylene glycol (PG), triethylamine, and sodium dihydrogen phosphate dodecahydrate were procured from Merck (Germany).
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