Human thrombin, PGE1, dimethyl sulfoxide (DMSO), ADP, fibrinogen, TXA2 analog U46619, ferric chloride (FeCl3), and all the reagents were purchased from Sigma (St. Louis, MO, United States). D -Phe-Pro-Arg-chloromethyl ketone (PPACK) was purchased from EMD Millipore (Billerica, MA, United States). Collagen-related peptide (CRP) was obtained from Dr. Richard Farndale (Department of Biochemistry, University of Cambridge, United Kingdom). Phycoerythrin (PE)-conjugated isotype control IgGs, rat monoclonal antibodies against mouse P-selectin, and activated αIIbβ3 (JON/A) were obtained from Emfret Analytics (Eibelstadt, Germany). Antibodies against phospho-PLCγ2 at Tyr759, phospho-PLCβ3 at Ser1105, phospho-Akt at Ser473, Total Akt, Total PLCγ2, Total PLCβ3, and actin were obtained from Cell Signaling (Danvers, MA, United States). Calcium dye (FLIPR Calcium Assay kit) was obtained from Molecular Devices (Sunnyvale, CA, United States).
Phycoerythrin pe conjugated isotype control iggs
Manufactured by Emfret Analytics
Sourced in Germany
Phycoerythrin (PE)-conjugated isotype control IgGs are a type of laboratory reagent used in flow cytometry and other immunological assays. They serve as a control to help determine the specificity of antibody binding in a sample. These reagents contain IgG antibodies that are labeled with the fluorescent dye phycoerythrin, which can be detected using flow cytometry or other fluorescence-based techniques.
Automatically generated - may contain errors
Lab products found in correlation
Total akt, by Cell Signaling Technology (2 mentions)
Dimethyl sulfoxide (dmso), by Merck Group (2 mentions)
Fibrinogen, by Merck Group (2 mentions)
Human thrombin, by Merck Group (2 mentions)
Jon a, by Emfret Analytics (2 mentions)
Total plcγ2, by Cell Signaling Technology (2 mentions)
Calcium dye flipr calcium assay kit, by Molecular Devices (2 mentions)
U46619, by Merck Group (1 mentions)
Adenosine diphosphate (adp), by Merck Group (1 mentions)
Ferric chloride fecl3, by Merck Group (1 mentions)
Rhodamine phalloidin, by Merck Group (1 mentions)
Total erk, by Cell Signaling Technology (1 mentions)
Human fibrinogen, by Merck Group (1 mentions)
Total syk, by Cell Signaling Technology (1 mentions)
β actin, by Cell Signaling Technology (1 mentions)
Phospho plcγ2, by Cell Signaling Technology (1 mentions)
Totalpi3k p85, by Cell Signaling Technology (1 mentions)
Phospho akt at ser473, by Cell Signaling Technology (1 mentions)
2 protocols using phycoerythrin pe conjugated isotype control iggs
1
Platelet Aggregation and Signaling Assays
2
Platelet Signaling Pathway Analysis
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Human thrombin, PGE1, rhodamine-phalloidin, dimethyl sulfoxide (DMSO), ADP, fibrinogen, human fibrinogen, ferric chloride (FeCl3), Acetylsalicylic acid (ASA), carboxymethylcellulose (CMC), and all the reagents were purchased from Sigma (St. Louis, MO, USA). Equine tendon collagen (type I) and ATP luciferin/luciferase reagent were obtained from Chrono-log (Havertown, PA, USA). CRP was obtained from Dr Richard Farndale (Department of Biochemistry, University of Cambridge, UK). Phycoerythrin (PE)-conjugated isotype control IgGs, rat monoclonal antibodies against mouse P-selectin, activated αIIbβ3 (JON/A) were from Emfret Analytics (Eibelstadt, Germany). Antibodies against phospho-Syk at Tyr525/526 (Cat# 2710S), phospho-PLCγ2 at Tyr1217 (Cat# 3871S), phospho-PI3K p85α/β at Tyr458/p55α/γ at Tyr199 (Cat# 4228S), phospho-Akt at Ser473 (Cat# 9271S), phospho-ERK at Thr202/Tyr204 (Cat# 4337S), Total Syk (Cat# 12358S), Total PLCγ2 (Cat# 3872S), Total PI3K p85 (Cat# 4249S), Total Akt (Cat# 9272S), Total ERK (Cat# 9102S), and β-actin (Cat# 4967S) were obtained from Cell Signaling (Danvers, MA, USA). Monoclonal antibodies against phospho-integrin β3 at Tyr 759 and Total integrin β3 were obtained from Santa Cruz (Santa Cruz, CA, USA). Calcium dye (FLIPR Calcium Assay kit) was from Molecular Devices (Sunnyvale, CA, USA).
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