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2 protocols using anti brg1 epncir111a

1

Histone Modification Analysis Protocol

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The following antibodies were used in this study: anti-KDM2A (12 (link)), anti-KDM2B (15 (link)), anti-BRG1 (EPNCIR111A, Abcam), anti-H3, anti-H3K36me1, anti-H3K36me2 (12 (link)), anti-H3K36me3, anti-H4 (L64C1, Cell Signalling), anti-H2AK119ub1 (D27C4, Cell Signalling), anti-H3K27me3 (45 (link)), anti-Rbp1-NTD (D8L4Y, Cell Signalling), anti-Rbp1-CTD-Ser5P (D9N5I, Cell Signalling), anti-Rbp1-CTD-Ser2P (E1Z3G, Cell Signalling). Anti-H3 and anti-H3K36me antibodies were prepared in-house by rabbit immunization with synthetic peptides (PTU/BS Scottish National Blood Transfusion Service) and antibodies were purified on peptide affinity columns.
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2

Comprehensive Antibody Characterization Protocol

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The primary antibodies used in this study were as follows: anti-BRG1 (EPNCIR111A) Abcam Cat# ab110641 (1:10,000); anti-Caspase3 (8G10), Cell Signaling Technology, Cat# 9665 (1:1000); anti-C-Caspase 3, Cell Signaling Technology, Cat# 9661 (1:1000); anti-PARP (46D11), Cell Signaling Technology, Cat# 9532 (1:1000); anti-Ki67 (B56), BD Biosciences, Cat# 550609 (1:500); anti-EpCAM (G8.8), BD Biosciences, Cat# 552370 (1:500); anti-ZO1, Invitrogen, Cat# 40-2200 (1:500); anti-8-OHd G, Abcam, Cat# ab48508 (1:500); anti-LC3A/B (D3U4C), Cell Signaling Technology, Cat# 12741 (1:1000); anti-ATG16L1 (D6A5), Cell Signaling Technology, Cat# 8089 (1:1000); anti-PCNA, Santa Cruz Biotechnology, Cat# SC-7909 (1:1000); anti-P-H3, Cell Signaling Technology, Cat# 9701 (1:1000); anti-E-Cadherin, Cell Signaling Technology, Cat# 3195T (1:1000); anti-DCLK1, Abcam, Cat# ab31704 (1:500); anti-Claudin1, Cell Signaling Technology, Cat# 4933T (1:1000); anti-ChgA, Abcam, Cat# ab715 (1:1000); anti-Hes1, Abcam, Cat# ab71559 (1:500). Raw data of immunoblotting can be found in the source data file.
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