Invertoskop 40c

Manufactured by Zeiss

Sourced in Germany

The Invertoskop 40C is a laboratory microscope designed for transmitted light applications. It provides high-quality optics and a stable platform for observation and analysis of prepared specimens. The instrument is equipped with a centrable illumination system and supports various objective magnifications to accommodate a range of sample types and research needs.

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Invertoskop 40C Microscope Invertoskop

3 protocols using invertoskop 40c

Organoid Growth Kinetics Analysis

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To examine the organoids growth over time, NC and CD organoids were plated at 5*10⁴ cells/mL and cultured for 7 days as previously described. Multiple fields per sample were acquired at 2, 4 and 7 days after plating using a bright field direct microscope (Invertoskop 40 C, Zeiss, Oberkochen, Germany). We calculated the area of the organoids (μm²) in the captured microfield using ImageJ Software (National Institute of Healthy, USA) and then averaged the areas based on the total number of the measured organoids.

Freire R., Ingano L., Serena G., Cetinbas M., Anselmo A., Sapone A., Sadreyev R.I., Fasano A, & Senger S. (2019). Human gut derived-organoids provide model to study gluten response and effects of microbiota-derived molecules in celiac disease. Scientific Reports, 9, 7029.

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Hypoxia-Induced Cell Migration Assay

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Cells were transiently transfected following the previously outlined protocol. After siRNA-lipid complexes were added to 24-well plates, 1×10⁵ PANC-1 cells in growth media were seeded into each well and incubated at 37°C for 24 h. Each well was then ‘scratched’ using a P1000 pipette tip down the middle of each well. Images of all wells were obtained. The plates were then placed in either 0.2% oxygen or maintained in normoxia and incubated for either 16 or 48 h. After incubation in normoxia or hypoxia, images of each well were captured using an inverted light microscope (Invertoskop 40C; Carl Zeiss AG) and analyzed using an ImageJ/Fiji plugin tool (25 (link)).

Chee N.T., Carriere C.H., Miller Z., Welford S, & Brothers S.P. (2022). Activating transcription factor 4 regulates hypoxia inducible factor 1α in chronic hypoxia in pancreatic cancer cells. Oncology Reports, 49(1), 14.

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Quantifying Ciliary Beat Frequency

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CF-HBE cultures were maintained at 37°C prior to recording of CBF. CBF was recorded by imaging the apical surface (X40 objective) with a camera in slow-motion capture of 720pixels at 240fps (frames-per second) mounted onto a Zeiss Invertoskop 40C (Zeiss) inverted microscope with a microscope adapter. An average of 4 counts per 10 second video was calculated using the Avidemux software (v2.6), which helped distinguish between cilia and vibration noise. Two independent researchers counted CBF, and analysis was performed using ImageJ v1.52a.

Rayner R.E., Wellmerling J., Osman W., Honesty S., Alfaro M., Peeples M.E, & Cormet-Boyaka E. (2020). In Vitro 3D culture lung model from expanded primary cystic fibrosis human airway cells. Journal of cystic fibrosis : official journal of the European Cystic Fibrosis Society, 19(5), 752-761.

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