Routine laboratory parameters were assessed using certified reagents and techniques with appropriate analytical properties in accredited clinical laboratories. Copeptin (CPP) serum concentration was determined using competitive ELISA principle by Human CPP (Copeptin) ELISA kit (Novus Biologicals, LLC, Centennial, CO, USA; Bio-Techne Ltd., Oxford, United Kingdom) on the automated ELISA analyzer Siemens BEP 2000 Advance (Siemens Healthcare Diagnostics Walpole, NJ, USA). Immunoglobulin A (IgA), G (IgG), and M (IgM), as well as complement component C3 (C3) and C4 (C4) serum concentrations were measured by immunoturbidimetric assay on Beckman Coulter DxC 700 AU analyzer (Beckman Coulter, Brea, CA, USA). DBC parameters (absolute number of neutrophilic granulocytes and lymphocytes) were determined using Abbott Cell Dyn Ruby (Abbott Diagnostics, Abbott Park, IL, USA) hematology analyzer in K2EDTA whole blood samples within 1 h after sampling. Neutrophil-to-lymphocyte ratio (NLR) was calculated by dividing the absolute number of neutrophils with the absolute number of lymphocytes.
Cell dyn ruby
Manufactured by Abbott
Sourced in United States, United Kingdom
The CELL-DYN Ruby is a hematology analyzer designed to perform complete blood count (CBC) and other related tests. It is capable of analyzing various blood cell parameters to provide clinically relevant information.
Automatically generated - may contain errors
Lab products found in correlation
Architect c8000 chemistry system, by Abbott (5 mentions)
Cobas 6000, by Roche (3 mentions)
Image 800, by Beckman Coulter (2 mentions)
Architect c16000 autoanalyzer, by Abbott (2 mentions)
Sta compact max, by Diagnostica Stago (2 mentions)
Dxc 700 au analyzer, by Beckman Coulter (1 mentions)
Bep 2000 advance, by Siemens (1 mentions)
Au400, by Olympus (1 mentions)
Duoset immunoassay development kit, by R&D Systems (1 mentions)
Cobas e601 analyzer, by Roche (1 mentions)
Cobas 6000 device, by Roche (1 mentions)
Sta compact max, by Stago (1 mentions)
Spectramax m3, by Molecular Devices (1 mentions)
Tni ultra, by Siemens (1 mentions)
Crp vario, by Abbott (1 mentions)
Liaison analyzer, by DiaSorin (1 mentions)
Facscanto 2, by BD (1 mentions)
Enzyme linked immunosorbent assay kit, by Elabscience (1 mentions)
Cs 2500, by Siemens (1 mentions)
Architect c8000, by Abbott (1 mentions)
58 protocols using cell dyn ruby
1
Routine Laboratory Biomarker Measurements
2
Metabolic Biomarker Profiling Protocol
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After fasting overnight, a venous blood sample (10 mL) was obtained. Total cholesterol, high density lipoprotein (HDL) cholesterol, low-density lipoprotein (LDL), triglycerides, glucose, and creatinine levels were measured with the Abbott Architect C16000 auto-analyzer (Abbott Park, Abbott Diagnostics, USA). Serum insulin was determined by a commercially available assay kit (Roche Cobas 6000, Roche-Hitachi Diagnostics, Germany). Insulin-resistance was estimated using the homeostasis model assessment as an index of insulin resistance (HOMA-IR), defined as follows: (fasting glucose mg/dL x fasting insulin µU/mL) / 405 (17 (link)). The cut-off value was taken as 2.7 for HOMA-IR (18 ). Hematologic parameters were measured by an automated hematology analyzer system (Abbott Cell-Dyn Ruby; Abbott Diagnostics, USA). Absolute cell counts were utilized to perform subsequent analyses. The serum concentration of C-reactive protein was evaluated using the high-sensitivity immunochemistry system, the nephelometric method (Image 800, Beckman Coulter, USA).
3
Hematological and Biochemical Profiles in Animal Chemotherapy
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The hematological and biochemical profiles of the animals from the groups NC, LDE-PTX175, LDE-PTX250, PTX175, and PTX250 were determined during all six cycles of chemotherapy (days of drug application or saline solution) as well as during the interval of cycles (on the 14th day) and after the last treatment cycle (21st day).
Biochemical profiles of the nonhuman primates were tracked all throughout the experimental period by analyzing the standard reagents such as alanine aminotransferase (ALT), aspartate aminotransferase (AST), gamma-glutamyl transferase (GGT), urea, creatinine, uric acid, glucose, cholesterol, high-density lipoprotein cholesterol, triglycerides, lipase, alkaline phosphatase, total protein, albumin, globulin, total bilirubin, and calcium using the Architect 5000® dry-chemical system (Abbott Diagnostics, Lake Forest, IL, USA), according to the method recommended by the manufacturers of the kits for each parameter analyzed. The hematological analysis, however, was performed using an automatic cell counter for veterinary use (Cell-Dyn Ruby®; Abbott Diagnostics).
Biochemical profiles of the nonhuman primates were tracked all throughout the experimental period by analyzing the standard reagents such as alanine aminotransferase (ALT), aspartate aminotransferase (AST), gamma-glutamyl transferase (GGT), urea, creatinine, uric acid, glucose, cholesterol, high-density lipoprotein cholesterol, triglycerides, lipase, alkaline phosphatase, total protein, albumin, globulin, total bilirubin, and calcium using the Architect 5000® dry-chemical system (Abbott Diagnostics, Lake Forest, IL, USA), according to the method recommended by the manufacturers of the kits for each parameter analyzed. The hematological analysis, however, was performed using an automatic cell counter for veterinary use (Cell-Dyn Ruby®; Abbott Diagnostics).
4
Admission Biomarker Analysis Protocol
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At the time of hospital admission, venous blood samples were tested. The Architect c8000 Chemistry System (Abbott Diagnostics, Abbott Park, Ill, USA) commercial kits were used to determine total cholesterol, fasting triglyceride, low-density lipoprotein cholesterol (LDL-C), and high-density lipoprotein cholesterol (HDL-C) levels. Using the automated hematology analyzer CELL-DYN Ruby (Abbott Diagnostics), total white blood (WBC) counts were calculated, including neutrophil lymphocyte counts, and expressed as 1000 cells/mm3. Calculations were also made for hemoglobin, albumin, and platelet count. Platelet-to-lymphocyte ratio (PLR) is computed as the number of platelets divided by the lymphocyte count, while neutrophil-to-lymphocyte ratio (NLR) is determined by dividing the neutrophil count by the lymphocyte count. Abbott Diagnostics’ biochemistry kits and Architect c8000 Chemistry System were used to measure the levels of C-reactive protein (CRP).
5
Biomarkers and Genetic Profiling
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30 mL of blood in ethylene diamine tetra acetic acid anticoagulant (EDTA) was collected from each individual for renal function analysis (urea and creatinine, Labtest, Lagoa Santa, MG, Brasil and Jaffe modified method [22 (link)], resp.), lipid profile (cholesterol, HDL and LDL cholesterol, automated method equipment Cell-Dyn Ruby Abbott Diagnostics), and markers of SIRS (CRP and albumin, Immulite 1000 immunoassay system, IL, USA, and colorimetric method in automated equipment Olympus AU400, Dallas, TX, USA, resp.) and DNA extraction was performed in order to study the polymorphisms of TGF-β and IFN-γ (Genotyping tray, One Lambda, Inc., 21001 Kittridge, St, Canoga Park, CA, USA).
6
Complete Blood Count Analysis
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From the remaining volume of the 2 mL sample aliquot, approximately 0.7 mL was used for complete blood count (CBC) measurements from a Cell-Dyn Ruby (Abbott Diagnostics, Abbott Park, IL) according to manufacturer instructions. Parameters evaluated from these blood counts were: platelet count, mean platelet volume, white blood cell count, lymphocyte count, hematocrit, red blood cell count, hemoglobin concentration, and mean corpuscular volume (the volume of each red blood cell).
7
Automated Hematology Analyzer Protocol
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An automated hematology analyzer (Cell-Dyn Ruby, Abbott Diagnostics) was applied for assessment of TNC count. The number of nucleated cells including erythroblasts is determined by using the mode “CBC+RRBC” (CBC = complete blood count; RRBC = resistant red blood cell). This routine measurement is checked on a workday basis by using Abbott’s quality controls (“Whole blood reference controls CellDynÒ 26 Control”) to ensure accurate cell counts. The TNC recovery is calculated by the following formula: (total cells after thawing/total cells after volume reduction)*100[%].
8
Blood Analysis for Renal Function and Lipid Profile
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A volume of 30mL of blood in ethylene diamine tetra-acetic acid (EDTA) anti-coagulant was collected from each individual for renal function analysis (blood urea nitrogen and creatinine; Labtest®, Lagoa Santa, MG, Brazil and the Jaffe modified method, respectively), lipid profile (HDL and LDL cholesterol; automated method equipment, Cell-Dyn Ruby; Abbott Diagnostics, Lake Forest, IL, USA), markers of systemic inflammatory response syndrome, CRP, albumin (Immunlite 1,000 immunoassay system, Erlangen, Germany), and the colorimetric method in automated equipment.
Charts were reviewed for clinical and epidemiological data and patients were allocated into Group Statin if they had prior statin use at least for the previous 6 months. Patients without prior statin use were allocated into Non-Statin Group.
Charts were reviewed for clinical and epidemiological data and patients were allocated into Group Statin if they had prior statin use at least for the previous 6 months. Patients without prior statin use were allocated into Non-Statin Group.
9
Biochemical and Hematological Profiling
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Venous blood samples were examined at hospital admission. Low‐density lipoprotein cholesterol (LDL‐C) and high‐density lipoprotein cholesterol (HDL‐C) levels were analyzed using the Architect c8000 Chemistry System (Abbott Diagnostics, USA) commercial kits. Complete white blood (WBC) counts, including neutrophil and lymphocyte counts, were measured using an automated hematology analyzer CELL‐DYN Ruby (Abbott Diagnostics) and expressed as ×1000 cells/mm3. Hemoglobin, hematocrit, platelet count, mean platelet volume (MCV), and red blood cell distribution width (RDW) were also calculated. Neutrophil to lymphocyte ratio (NLR) was calculated by dividing the neutrophil count by the lymphocyte count, and platelet to lymphocyte ratio (PLR) was calculated as the number of platelets divided by the lymphocyte count. C‐reactive protein (CRP) levels were analyzed using biochemistry kits (Abbott Diagnostics) and an Architect c8000 Chemistry System (Abbott Diagnostics) device.
10
Comprehensive Blood Profiling Protocol
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Venous blood specimens were analyzed upon admission to the hospital. Total white blood cell count and neutrophil, lymphocyte, monocytes, eosinophil, and basophil counts were measured using a device (CELL-DYN Ruby; Abbott Diagnostics, Abbott Park, IL) and given as x 103 cells/mm3. Hemoglobin, hematocrit, and thrombocyte numbers were also measured. Creatinine, urea, andCRP levels were analyzed using biochemistry kits (Abbott Diagnostics) and an Architect c8000 Chemistry System (Abbott Diagnostics) machine. Total cholesterol, fasting triglyceride, HDL, low-density lipoprotein (LDL), and fasting blood glucose plasma concentrations were measured by the enzymatic chemical clearing process operating Cobas 6000 (Roche Diagnostics GmbH, Mannheim, Germany). NLR, PLR, MLR and MHR were calculated.
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