Total Cell Extracts (TCEs) were prepared and resolved as previously described [13 (link)]. The following Abs were employed: anti-Ras (OP40, 1:500 diluition; Calbiochem, Cambridge, UK), anti-E1A (M58 clone, 1:500 diluition; BD Biosciences, San Jose, CA, USA), anti-GAPDH (1:1000 diluition; Santa Cruz Biotechnology, Santa Cruz, CA, USA), anti-HIPK2 (kindly provided by Dr. L. Shmidtz); anti-GFP (1:500 diluition; Santa Cruz Biotechnology, Santa Cruz, CA, USA); anti-alpha-tubulin and anti-Actin (1:1000 diluition; Immunological Science, Rome, Italy); anti-HRP-conjugated goat anti-mouse and anti-rabbit (Bio-Rad Laboratories, Hercules, CA, USA). Immunoreactivity was determined using the ECL-chemiluminescence reaction (Amersham, Piscataway, NJ, USA) following the manufacturer's instructions.
Ecl chemiluminescence reaction
Manufactured by Cytiva
Sourced in United States
ECL-chemiluminescence reaction is a laboratory technique used to detect and quantify specific proteins in a sample. It employs a chemical reaction that produces light, which can be measured and used to determine the presence and amount of the target protein. This method is commonly used in Western blotting and other immunoassays.
Automatically generated - may contain errors
2 protocols using ecl chemiluminescence reaction
1
Immunoblotting Assay for Protein Expression
2
Immunoblotting of MCF7 Cell Extracts
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Preparation of whole
cell extracts from MCF7 cells and immunoblotting were carried out
as described.6 (link),40 (link),41 (link) Immunoreactivity was detected using ECL chemiluminescence reaction
(Amersham) under ChemiDoc MP system (Bio-Rad, USA).
cell extracts from MCF7 cells and immunoblotting were carried out
as described.6 (link),40 (link),41 (link) Immunoreactivity was detected using ECL chemiluminescence reaction
(Amersham) under ChemiDoc MP system (Bio-Rad, USA).
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