The HPLC was used on a Shimadzu LC-20AVP system with two LC-20AT solvent delivery units, an SPD-20A UV-vis detector, a CTO-10ASVP column oven (Shimadzu, Kyoto, Japan), a T2000P workstation (Shenyang, China) and a reversed-phase C18 column (250 × 4.6mm, 5μm, DiamodsilTM). The conditions for the HPLC detection of silymarin were as follows: solvent A, methanol; solvent B, water (1 ‰ formic acid); gradient (A%), initial 4 min 43 %, 4-25 min 43-70 %, 25-30 min 70 %, 30.01 min 43 %, 40 min, stop; flow rate, 1mL/min; injection volume, 10μL, wavelength, 288 nm; column temperature,40°C. A sample chromatogram from the extraction of silymarin is shown in Figure 1(Fig. 1) .
Lc 20avp system
Manufactured by Shimadzu
Sourced in Japan
The Shimadzu LC-20AVP system is a high-performance liquid chromatography (HPLC) instrument designed for analytical applications. It features a quaternary pump, an autosampler, a column oven, and a variety of detectors to provide comprehensive analytical capabilities. The system is capable of performing diverse chromatographic separations and analyses, but a detailed description of its intended use would require more specific information.
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Lab products found in correlation
Lc 20at solvent delivery units, by Shimadzu (1 mentions)
Cto 10asvp column oven, by Shimadzu (1 mentions)
Spd 20a uv vis detector, by Shimadzu (1 mentions)
Hypersil goldtm column, by Thermo Fisher Scientific (1 mentions)
Lc 20ad solvent, by Shimadzu (1 mentions)
Spd 20a uv, by Shimadzu (1 mentions)
Cbm 20a, by Shimadzu (1 mentions)
4 protocols using lc 20avp system
1
HPLC Analysis of Silymarin
2
HPLC Analysis of Bioactive Compounds
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Column: Hypersil GOLDTM column (5 μm, 250 mm × 4.6 mm) (Thermo Scientific, California, USA); equipment: LC-20AVP system (SHIMADZU, Kyoto, Japan); wavelength: 273 nm; column oven: 40 °C; mobile phase A: 0.1% (v/v, mL/mL) acetic acid in ultrapure water; mobile phase B: methanol; injection volume: 4 μL; elution velocity: 0.8 mL min−1; elution program: 80% A (0–9 min), 80-5% A (9–10 min), 5% A (10–30 min), 5-1% A (30–31 min), 1% A (31–35 min), 1–80% A (35–36 min) and 80% A (36–45 min).
3
Nanoparticle Encapsulation Efficiency Determination
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The encapsulation efficiency was determined by ultrafiltration. The samples were placed in an ultrafiltration device (100 kMWCO; Sartorius, Göettingen, Germany) and centrifuged at 3,500 rpm for 10 min at room temperature to isolate the free drug. The same volume of each sample was dissolved in acetonitrile to obtain the total amount of drug. The concentrations of rotigotine and coumarin-6 were measured with a high-performance liqid chromatography (HPLC) system (LC-20A VP system; Shimadzu, Kyoto, Japan), while the concentration of DiR was measured by UV spectrophotometry. The encapsulation efficiency and drug loading capacity of the NPs were calculated using the following equation:
4
HPLC Analysis of Que Compound
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The HPLC equipment consisted of a Shimadzu LC-20Avp system including two LC-20AD solvent delivery units, and SPD-20 A UV–vis photodiode array detector, and CBM-20 A system controller, a CTO-20 A column oven, a DGU-20A3 degaser, and injection valve with 20 µL loop. Data were processed using the Shimadzu Class VP 5.3 HPLC data system on a Pentium II 400 PC compatible computer. The column was an Inertsil ODS-4 (50 mm × 2.1 mm ×3 µm). The column temperature was 30 °C. Elution was performed using a mobile phase made up of pure water:acetonitrile:2-propanol (70:22:8) at a flow rate of 0.05 mL/min. Chromatograms were recorded at 370 nm. Que was identified by comparing retention times and UV–vis spectra, with an authentic compound (Carari et al. 2003 (link)).
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