Rabbit anti arl13b antibody

Manufactured by Proteintech

Sourced in United States

Rabbit anti-ARL13B antibody is a primary antibody that specifically targets the ARL13B protein. ARL13B is a small GTPase that plays a role in the formation and function of primary cilia. This antibody can be used for the detection and analysis of ARL13B expression in various experimental applications.

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Lab products found in correlation

Goat serum, by Thermo Fisher Scientific (1 mentions) Vectashield mounting medium, by Vector Laboratories (1 mentions) Fv1000 confocal microscope, by Olympus (1 mentions) 510 meta, by Zeiss (1 mentions) Vectashield containing dapi, by Vector Laboratories (1 mentions) Dimethyl sulfoxide (dmso), by Thermo Fisher Scientific (1 mentions) Rabbit anti pcm1, by Fortis Life Sciences (1 mentions) Rabbit anti hdac6, by Cell Signaling Technology (1 mentions) Mouse anti tuj1, by R&D Systems (1 mentions) Acy 738, by MedChemExpress (1 mentions) Chicken anti gfp, by Abcam (1 mentions)

Close spelling variants of this product

ARL13B (41 citations) Anti-Arl13b (22 citations) Rabbit anti-ARL13B (13 citations) Anti-Arl13b antibody (4 citations) ARL13B antibody (3 citations) Anti-TM (2 citations)

3 protocols using rabbit anti arl13b antibody

Cilia Labeling in Transfected NIH-3T3 Cells

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NIH-3T3 cells transiently transfected with GFP-tagged constructs were grown on collagen I-coated culture slides (BD Biosciences), and 48 h after transfection cells were fixed with 4% PFA at room temperature (RT) for 10 minutes. Following one wash with PBS+Ca²⁺, cells were blocked in blocking buffer (PBS+Ca²⁺ + 1% goat serum (Invitrogen) and 0.1% Triton-X-100) for 30 minutes at RT. Cilia were labeled with rabbit anti-Arl13b antibody (1:500; ProteinTech) overnight in blocking buffer. Following primary antibody incubation, cells were washed three times in blocking buffer and incubated with Alexa 546 fluorophore (red) conjugated anti-rabbit secondary antibody (Invitrogen) in blocking buffer for 1 h at RT. After a single rinse, cell nuclei were stained with DAPI (USB Affymetrix) for 10 min. Following two additional rinses, cells were mounted onto slides using Vectashield mounting medium (Vector Laboratories) and sealed. Images were captured using an Olympus FV1000 confocal microscope (Olympus).

Giddens M.M., Wong J.C., Schroeder J.P., Farrow E.G., Smith B.M., Owino S., Soden S.E., Meyer R.C., Saunders C., LePichon J.B., Weinshenker D., Escayg A, & Hall R.A. (2017). GPR37L1 Modulates Seizure Susceptibility: Evidence from Mouse Studies and Analyses of a Human GPR37L1 Variant. Neurobiology of disease, 106, 181-190.

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Cilia Analysis in Patient Cell Lines

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Antibodies that were used in this study were: rabbit anti-ARL13B antibody (Proteintech Group, Chicago, Illinois, USA), mouse anti-gamma tubulin (clone GTU-88, Sigma), Alexa Fluor secondary donkey antirabbit and donkey antimouse antibodies (Invitrogen).
For cilia analysis, cells were grown in coverslips until 70% confluent, serum-starved for 24 h and fixed using ice-cold methanol for 10 min. After three washes in PBS, cells were blocked with 2% donkey serum and 2% BSA in PBS, and incubated in primary antibodies overnight at 4°C. After washing, samples were incubated in appropriate secondary antibodies, washed and mounted in Vectashield containing DAPI (Vector Laboratories). Cells were imaged with a Zeiss 510 META confocal laser-scanning microscope. Optical sections were collected from the xy plane and merged into maximum projection images. A total of 200 cells were analysed per cell line. Non-parametric t test was used to compare control and patient cells.

Malicdan M.C., Vilboux T., Stephen J., Maglic D., Mian L., Konzman D., Guo J., Yildirimli D., Bryant J., Fischer R., Zein W.M., Snow J., Vemulapalli M., Mullikin J.C., Toro C., Solomon B.D., Niederhuber J.E., Gahl W.A, & Gunay-Aygun M. (2015). Mutations in human homologue of chicken talpid3 gene (KIAA0586) cause a hybrid ciliopathy with overlapping features of Jeune and Joubert syndromes. Journal of medical genetics, 52(12), 830-839.

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Antibody Validation for Cellular Imaging and WB

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Primary antibodies used for immunocytochemistry (ICC) and WB included mouse anti-aaTub (1:10,000; Sigma, St. Louis, MO, USA; Cat #T6793), rabbit anti-ARL13B antibody (1:5000; Proteintech, Rosemont, IL, USA; Cat #17711-1-AP), mouse anti-TUJ1 (1:2000; R&D Systems, Minneapolis, MN, USA; Cat #NL1195V), chicken anti-Ki-67 (1:5000; Encor, Gainesville, FL, USA; Cat# CPCA-Ki67), chicken anti-GFAP (1:1000; Encor, Gainesville, FL, USA; Cat# CPCA-GFAP), mouse anti-GAPDH (1:10,000; Encor, Gainesville, FL, USA; Cat# MCA-1D4), chicken anti-GFP (1:5000; Abcam, Cambridge, UK; #ab13970), rabbit anti-HDAC6 (1:1000; Sigma, St. Louis, MO, USA; Cat #H2287), rabbit anti-HDAC6 (1:1000; Cell Signaling, Danvers, MA, USA; Cat #7558S), mouse anti-KIF3A (BD Biosciences, San Jose, CA, USA; Cat #611508), and rabbit anti-PCM1 (1:1000; Bethyl Laboratories, Montgomery, TX, USA; Cat# A301-150A). ACY-1215 (APEXbio, Houston, TX, USA; Cat #A4083) and ACY-738 (MedChemExpress, Monmouth Junction, NJ, USA; Cat #1375465-91-0) were dissolved in 100% DMSO (Fisher Scientific, Waltham, MA, USA; Cat # D128-500) to produce a stock concentration of 10 mM. cDNA vectors pCMV-EGFP or pCMV-mycEGFP:HDAC6[#NM_001321225.2] were designed and obtained from Vectorbuilder (Vectorbuilder.com (accessed on 29 March 2021)).

Shi P., Hoang-Minh L.B., Tian J., Cheng A., Basrai R., Kalaria N., Lebowitz J.J., Khoshbouei H., Deleyrolle L.P, & Sarkisian M.R. (2021). HDAC6 Signaling at Primary Cilia Promotes Proliferation and Restricts Differentiation of Glioma Cells. Cancers, 13(7), 1644.

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