Electroporation was carried out to introduce synthetic MiRNA mimics or inhibitors (90 pmol/test) to C2C12 cells by a Nucleofector™ 2b (Lonza, Basel, Switzerland) with an Amaxa Cell Line Nucleofector Kit V (Lonza), according to the manufacturer’s instructions. After electroporation, the cells were cultured in differentiation medium at 30 °C. MiRNA mimics purchased from Sigma-Aldrich are as follows (catalog numbers are indicated in parentheses):
miR-1 (HMI0046), miR-133a (HMI0196), miR-133b (HMI0198), miR-26a (HMI0415), miR-125b (HMI0112), miR-199a-3p (HMI0338), miR-214 (HMI0379), let-7e (HMI0013), and negative control 1 (HMC0002).
Inhibitors purchased from QIAGEN are as follows:
miR-199a-3p inhibitor (YI04100433) and inhibitor control (negative control A; YI00199006).
miR-1 (HMI0046), miR-133a (HMI0196), miR-133b (HMI0198), miR-26a (HMI0415), miR-125b (HMI0112), miR-199a-3p (HMI0338), miR-214 (HMI0379), let-7e (HMI0013), and negative control 1 (HMC0002).
Inhibitors purchased from QIAGEN are as follows:
miR-199a-3p inhibitor (YI04100433) and inhibitor control (negative control A; YI00199006).