Cells or cells growing on collagen were fixed by 4% PFA and then washed three times with PBS. The samples were permeabilized and blocked by PBS containing 0.3% Triton X‐100 (Sigma, T8787) and 3% bovine serum albumin (BSA) (Amresco) for 1 h. The following primary antibodies were used: anti‐c‐Fos (Abcam, ab190289), anti‐MMP9 (Thermo, MA515886), anti‐Paxillin (Abcam, ab32115), anti‐collagen IV (Abcam, ab6586), anti‐CD31 (Abcam, ab9498), anti‐CD32 (Abcam, ab131051), anti‐LYVE1 (Abcam, ab14917), anti‐FVIII (Abcam, ab275376), and anti‐STAB2 (Abcam, ab121893). Cells were washed three times with PBS. The fluorescent secondary antibody was incubated for 1 h. Cells were washed three times with PBS.
Ab121893
Manufactured by Abcam
Sourced in United Kingdom
Ab121893 is a laboratory product offered by Abcam. It is a piece of equipment designed for use in scientific research and laboratory settings. The core function of this product is to perform a specific task or procedure, but without further details on its intended use or capabilities.
Automatically generated - may contain errors
Lab products found in correlation
A10042, by Thermo Fisher Scientific (2 mentions)
Powerix70, by Olympus (2 mentions)
Ab150107, by Abcam (2 mentions)
Anti mmp9, by Thermo Fisher Scientific (1 mentions)
Ab14917, by Abcam (1 mentions)
Ab32115, by Abcam (1 mentions)
Ab275376, by Abcam (1 mentions)
Ab9498, by Abcam (1 mentions)
Ab6586, by Abcam (1 mentions)
A 21424, by Thermo Fisher Scientific (1 mentions)
Ls b2138, by LifeSpan BioSciences (1 mentions)
Ab24590, by Abcam (1 mentions)
Ab150129, by Abcam (1 mentions)
Ab135813, by Abcam (1 mentions)
Fluorescence mounting medium, by Agilent Technologies (1 mentions)
Ethylene glycol tetraacetic acid (egta), by Merck Group (1 mentions)
Hepes, by Merck Group (1 mentions)
Mgcl2, by Merck Group (1 mentions)
Pipes, by Merck Group (1 mentions)
Jc70a, by Agilent Technologies (1 mentions)
4 protocols using ab121893
1
Immunofluorescence Staining of Cells
2
Immunofluorescence Staining of Endothelial Markers
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Samples were stained following the protocol previously described [14] . The antibodies used were: CD144 (rabbit, LS-B2138, LifeSpan Biosciences), Stabilin-2 (rabbit, ab121893, abcam), Stabilin-1 (mouse, H00023166-M05, Abnova), CD31-PE (mouse, 555446,BD Bioscience), anti-rabbit Alexa Fluor 568 (donkey, A10042, Thermofisher), anti-mouse Alexa Fluor 647 (donkey, ab150107, abcam) and antimouse (goat, A21424, Life Technologies). All fluorescent images were taken using a confocal microscope (PowerIX70, Olympus).
3
Multicolor Immunofluorescence Staining
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Staining was performed following the protocol detailed in supplementary material File 1. The antibodies used in this study were goat anti-albumin (A80–129A, Bethyl), rabbit anti-CYP3A4 (ab135813, abcam), rabbit anti-Stabilin-2 (ab121893, abcam), mouse anti-PECAM-1 (ab24590, abcam), donkey anti-goat Alexa Fluor 488 (ab150129, abcam), donkey anti-rabbit Alexa Fluor 568 (A10042, Thermofisher), and donkey anti-mouse Alexa Fluor 647 (ab150107, abcam). Fluorescent images were captured using a confocal microscope (PowerIX70, Olympus).
4
Multi-marker Immunofluorescence Staining
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Cells were fixed with 4% paraformaldehyde in PBS, and permeabilised with 0.3% Triton-X detergent in PHEM buffer (10 mM PIPES, 25 mM HEPES, 10 mM EGTA, 2 mM MgCl2, pH 6.9, all from Sigma-Aldrich). Primary antibodies against CD31 (1:50, JC70A, Dako, Glostrup, Denmark), LYVE-1 (1:100, AF2089, R&D Systems, MN, USA) CD32B (1:100, ab45143, Abcam, Cambridge, UK), Stabilin-2 (1:100, ab121893, Abcam), and Factor VIII (1:100, SAF8C-AP, Affinity Biologicals, ONT, Canada) were applied for 1 h, followed by secondary antibody for 30 min (either Alexa-Fluor 488 or 594 conjugated goat anti-mouse, goat anti-rabbit, or goat anti-sheep, all at 1:200, Thermo Fisher Scientific, MA, USA). After nuclear staining with DAPI, slides were mounted with fluorescence mounting medium (Dako) and a glass coverslip. Cells were imaged using a fluorescence microscope (Olympus BX61, Olympus, Tokyo, Japan).
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