Gold (III) chloride trihydrate (HAuCl4·3H2O, ≥99.9% trace metals basis), tri-sodium citrate (for molecular biology, ≥99%), tannic acid (ACS reagent), SH-PEG2k-NH2, Foetal Bovine Serum (FBS), Amphotericin-β, Penicillin-Streptomycin, and Dulbecco’s Modified Eagle’s Medium (D5796) were all acquired from Sigma-Aldrich, Johannesburg, South Africa. TrypLE™ Select Enzyme (1X) (12563-029) ThermoFisher, Johannesburg, South Africa). Hoechst 33258, Caspase 3, and 9 Multiplex Assay kit (Ab219915) as well as 96-well ultra-low attachment plates (174929) were purchased from ThermoFisher, Johannesburg, South Africa. Annexin V/PI apoptosis detection kit (556570) was procured from BD Biosciences, The Scientific Group, Johannesburg, South Africa).
Sh peg2k nh2
Manufactured by Merck Group
SH-PEG2k-NH2 is a heterobifunctional polyethylene glycol (PEG) derivative with a thiol (SH) group on one end and a primary amine (NH2) group on the other end. It is commonly used as a linker or spacer molecule in various bioconjugation and surface functionalization applications.
Automatically generated - may contain errors
Lab products found in correlation
Tannic acid, by Merck Group (3 mentions)
96 well ultra low attachment plate, by Thermo Fisher Scientific (2 mentions)
Dulbecco s modified eagle s medium d5796, by Merck Group (2 mentions)
Penicillin streptomycin, by Merck Group (2 mentions)
Trisodium citrate, by Merck Group (2 mentions)
Gold 3 chloride trihydrate, by Merck Group (2 mentions)
Annexin 5 pi apoptosis detection kit, by BD (2 mentions)
Foetal bovine serum (fbs), by Merck Group (1 mentions)
Tryple select enzyme 1x, by Thermo Fisher Scientific (1 mentions)
Amphotericin b, by Merck Group (1 mentions)
Hoechst 33258, by Thermo Fisher Scientific (1 mentions)
Accumax solution, by Merck Group (1 mentions)
Triton x 100, by Merck Group (1 mentions)
Caco 2 cellonex cat ss1402 ccac fl ccac c, by American Type Culture Collection (1 mentions)
3 protocols using sh peg2k nh2
1
Gold Nanoparticles Synthesis and Characterization
2
Nanoparticle-Mediated Cell Apoptosis Analysis
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Gold (III) chloride trihydrate (HAuCl4·3H2O, ≥99.9% trace metals basis), tri-sodium citrate (for molecular biology, ≥99%), tannic acid (ACS reagent), SH-PEG2k-NH2, Triton X-100 (T9284), Accumax™ solution (A7089), Foetal Bovine Serum (FBS), Amphotericin-β, Penicillin-Streptomycin, and Dulbecco’s Modified Eagle’s Medium (D5796) were all procured from Sigma-Aldrich, Johannesburg, South Africa. TrypLETM Select Enzyme (1X) (12563-029) ThermoFisher, Johannesburg, South Africa). Caspase 3, 8, and 9 Multiplex Assay kit (Ab219915) and 96-well ultra-low attachment plates (174929) were also purchased from ThermoFisher, Johannesburg, South Africa. Annexin V/PI apoptosis detection kit (556570) was procured from BD Biosciences, The Scientific Group, Johannesburg, South Africa).
3
Synthesis and Characterization of PEGylated Gold Nanoparticles
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Gold (III) chloride trihydrate (HAuCl 4 .3H 2 O, ≥99.9% trace metals basis, Mw = 393.83 g/mol), tri-sodium citrate (for molecular biology, ≥99%), tannic acid (ACS reagent), SH-PEG2k-NH 2 (HCl salt, average Mn: 2000) and phosphate-buffered saline (PBS, 10× concentrate, BioPerformance Certified, suitable for cell culture) were purchased from Sigma Aldrich. Zinc phthalocyanine tetrasulfonate (ZnPcS 4 PS) was purchased from Santa Cruz ® Biotechnology sc-264509A). Human colon cancer cells (CaCo-2 Cellonex Cat SS1402 CCAC-FL; CCAC-C) were purchased from the (American Type Culture Collection, Manassas, VA, USA). Monoclonal Anti-GCC Ab (Abcam: ab122404). 96-well ultra-low attachment plates (174,929) were acquired from ThermoFisher, Johannesburg, South Africa. All chemicals and reagents utilised in the study were of analytical grade. Millipore water with a resistance of 18.25 MΩ cm was utilised to prepare aqueous solutions. All glassware were autoclaved and rinsed with aqua regia solution before synthesis.
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