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Alexafluor 647 c2 maleimide free af

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AlexaFluor™ 647 C2 maleimide (Free AF) is a fluorescent labeling reagent used for the covalent attachment of fluorescent dyes to proteins and other biomolecules. The maleimide functional group allows for specific labeling of free sulfhydryl groups (cysteine residues) on target molecules.

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Lab products found in correlation

Molecular biology grade water, by Merck Group (2 mentions) Ssdna oligonucleotide, by Integrated DNA Technologies (2 mentions) Alexafluor 647 labeled ssdna oligonucleotides, by Integrated DNA Technologies (2 mentions) Ultrapure equimolar deoxynucleotide triphosphate dntp sodium salt solution, by New England Biolabs (2 mentions) Sodium chloride nacl magnesium chloride mgcl2, by Merck Group (2 mentions)

2 protocols using alexafluor 647 c2 maleimide free af

1

Fluorescent DNA Oligonucleotide Synthesis

Check if the same lab product or an alternative is used in the 5 most similar protocols
ssDNA oligonucleotides and AlexaFluor™ 647-labeled ssDNA oligonucleotides were purchased from Integrated DNA Technologies Inc. Full oligonucleotide sequences can be found in the accompanying Supporting Information (SI) file. Ultrapure equimolar deoxynucleotide triphosphate (dNTP) sodium salt solution was purchased from New England Biolabs Inc. (Catalog #N0447S) as an equimolar mixture of 10 mM dATP, dCTP, dGTP and dTTP giving a final dNTP concentration of 40 mM. AlexaFluor™ 647 C2 maleimide (Free AF) was purchased from ThermoFisher Scientific (Catalog #A20347). All other chemicals including 97% 1, 1′-diethyl-2, 2′-cyanine iodide (PIC, CAS #:977-96-8); molecular biology-grade water; sodium chloride (NaCl); magnesium chloride (MgCl2); and TRIS (2-Amino-2-(hydroxymethyl)propane-1, 3-diol; pH 7.0) were sourced from Sigma-Aldrich.
Chiriboga M., Green C.M., Mathur D., Hastman D.A., Melinger J.S., Veneziano R., Medintz I.L, & Díaz S.A. (2023). Structural and optical variation of pseudoisocyanine aggregates nucleated on DNA substrates. Methods and applications in fluorescence, 11(1), 10.1088/2050-6120/acb2b4.
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2

Fluorescent DNA Oligonucleotide Synthesis

Check if the same lab product or an alternative is used in the 5 most similar protocols
ssDNA oligonucleotides and AlexaFluor™ 647-labeled ssDNA oligonucleotides were purchased from Integrated DNA Technologies Inc. Full oligonucleotide sequences can be found in the accompanying Supporting Information (SI) file. Ultrapure equimolar deoxynucleotide triphosphate (dNTP) sodium salt solution was purchased from New England Biolabs Inc. (Catalog #N0447S) as an equimolar mixture of 10 mM dATP, dCTP, dGTP and dTTP giving a final dNTP concentration of 40 mM. AlexaFluor™ 647 C2 maleimide (Free AF) was purchased from ThermoFisher Scientific (Catalog #A20347). All other chemicals including 97% 1, 1′-diethyl-2, 2′-cyanine iodide (PIC, CAS #:977-96-8); molecular biology-grade water; sodium chloride (NaCl); magnesium chloride (MgCl2); and TRIS (2-Amino-2-(hydroxymethyl)propane-1, 3-diol; pH 7.0) were sourced from Sigma-Aldrich.
Chiriboga M., Green C.M., Mathur D., Hastman D.A., Melinger J.S., Veneziano R., Medintz I.L, & Díaz S.A. (2023). Structural and optical variation of pseudoisocyanine aggregates nucleated on DNA substrates. Methods and applications in fluorescence, 11(1), 10.1088/2050-6120/acb2b4.
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