Sisrc

Predesigned Src-targeting siRNA (siSrc) and control siRNA (siCon) were purchased from Santa Cruz. The cells were transfected with 100 nM siRNA while using Lipofectamine RNAiMAX (Invitrogen, Waltham, MA, USA) for 5 h, cultured in medium containing 0.5% FBS, and harvested ~48 h after transfection.

Dasatinib and Rapamycin were purchased from Selleck Chemical (Shanghai, China). Primary antibodies against phospho-Src (Tyr416), Src, phospho-mTOR (Ser2448), mTOR, phospho-PI3K (Tyr458), PI3K p85, phospho-Akt (Thr308), Akt, phospho-FoxO1 (Ser256), phospho-FoxO3a (Ser253), phospho-4E-BP1 (Thr37/46), 4E-BP1, Cdk2, Cdk4, Cdk6, and Alexa Fluor 555 conjugated antibody were obtained from Cell Signal Technologies (Shanghai, China). Primary antibodies against CDK inhibitor proteins including p16, p19, p21, p27, and β-tubulin were purchased from Santa Cruz Biotechnology (Shanghai, China). Antibodies against phospho-p70S6K (Thr389/412), p70S6K, were purchased from SAB Signalway (College Park, MD, USA). The Secondary HRP- or FITC- conjugated antibodies, si-mTOR, si-Src, and transfection reagents were purchased from Santa Cruz Biotechnology (Shanghai, China).