Porcine pancreatic α amylase

The solvents used for extraction of plant material were of HPLC grade. Tris buffer were purchased from Merck (Darmstadt, Germany), porcine pancreatic lipase, ascorbic acid, starch, acarbose, DPPH (2,2-diphenyl-1-picrylhydrazyl), PNPP (p-nitrophenyl palmitate) and orlistat were bought from Sigma-Aldrich (USA) and porcine pancreatic α-amylase from MP Biochemicals (Illkirch, France). All the chemicals used in the experiments were of analytical grade.

2,2-Diphenyl-1-picrylhydrazyl (DPPH) (Sigma Aldrich, Germany) and ((s)-(-)-6 hydroxy-2,5,7,8-tetramethychroman-2-carboxylic acid) (Sigma Aldrich, Denmark). n-hexane (Frutarom Ltd, Israel). Methanol (Lobachemie, India), The solvents used for the extraction of plant material were of HPLC grade. Sigma-Aldrich (USA) provided porcine pancreatic lipase, starch, acarbose, DPPH (2,2-diphenyl-1-picrylhydrazyl), PNPP (p-nitrophenyl palmitate), and orlistat, while MP Biochemicals (Illkirch, France) provided porcine pancreatic α-amylase. All the chemicals used in the experiments were of analytical grade. O. basilicum seeds were obtained from different regions in Palestine. The plant seeds were characterized in the Pharmacy Department at An-Najah National University. The plant material was collected in accordance with the WHO Guidelines for the Assessment of Herbal Medicines and Legislation.
The rotary evaporator (Heidolph OB2000, VV2000, Germany), the grinder (Moulinex model, Uno, China), the balance (Radwag, AS 220/c/2, Poland), and the filter papers (Machrery-Nagel, MN 617, Whatman no. 1, USA) were all used.