Anti gapdh ab

Manufactured by Cell Signaling Technology

Sourced in United States

Anti-GAPDH Ab is a primary antibody product that specifically recognizes the GAPDH (Glyceraldehyde-3-Phosphate Dehydrogenase) protein. GAPDH is a well-established reference protein commonly used as a loading control in various biochemical and cell biology applications.

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Lab products found in correlation

Anti akt ab, by Cell Signaling Technology (2 mentions) Ab32351, by Abcam (1 mentions) Ab181469, by Abcam (1 mentions) Ab215191, by Proteintech (1 mentions) Ab51608, by Abcam (1 mentions) Lipofectamine rnaimax reagent, by Thermo Fisher Scientific (1 mentions) Opti mem medium, by Thermo Fisher Scientific (1 mentions) Si gsdme, by Abcam (1 mentions) Anti mlkl, by Abcam (1 mentions) Rabbit anti rip3 ab, by Abcam (1 mentions) Rabbit anti caspase 8, by Abcam (1 mentions) Anti caspase 1 p20, by Adipogen (1 mentions) Mouse anti nlrp3, by Adipogen (1 mentions) Polyvinylidene fluoride membrane, by Merck Group (1 mentions) Anti α sma bm0002, by Boster Bio (1 mentions) Ikk 16, by Selleck Chemicals (1 mentions) Anti erk ab, by Cell Signaling Technology (1 mentions) Anti p38 ab, by Cell Signaling Technology (1 mentions) Anti nf κb p65 ab, by Cell Signaling Technology (1 mentions) Anti phospho nf κb p65 ab, by Cell Signaling Technology (1 mentions)

5 protocols using anti gapdh ab

Targeting GSDME for Cell Fate Regulation

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Lipofectamine RNAiMAX reagent and opti-MEM medium were purchased from Thermo Fisher Scientific (United States). Small interfering RNAs (siRNAs) including GSDME (si-GSDME#1, stB0006132A; si-GSDME#2, stB0006132B; si-GSDME#3, stB0006132C) and control siRNAs (si-NC, siN0000001-1-10) were obtained from RiboBio (Guangzhou, China). The corresponding target sequences for GSDME silencing were si-GSDME#1, CCCACTGCTTCTTTGTATA; si-GSDME#2, CAAGCAGCTGTTTATGACA and si-GSDME#3, GGTCCTATTTGATGATGAA. Primary antibodies including anti-human GSDME monoclonal ab (Abcam, ab215191), anti-human GSDME polyclonal ab (Proteintech, 13075-1-AP), anti-human-Thy1/CD90 ab (Abcam, ab181469), anti-human HIF-1α ab (Abcam, ab51608), anti-human Caspase-3 ab (Abcam, ab32351), anti-human IL-6 ab (Abcam ab9324) and anti-GAPDH ab (Cell Signaling Technology, #5174) were used in this study.

Wu T., Zhang X.P., Zhang Q., Zou Y.Y., Ma J.D., Chen L.F., Zou Y.W., Xue J.M., Ma R.F., Chen Z, & Dai L. (2022). Gasdermin-E Mediated Pyroptosis—A Novel Mechanism Regulating Migration, Invasion and Release of Inflammatory Cytokines in Rheumatoid Arthritis Fibroblast-like Synoviocytes. Frontiers in Cell and Developmental Biology, 9, 810635.

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Immunoblotting Analysis of Kidney and Podocyte Proteins

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For immunoblotting analysis, electrophoresis of kidney cell lysates or cell lysates from the NZM2328 podocyte cell line was conducted with 10% running gels and 5% stacking gels. Proteins from the cell lysates were then transferred onto a polyvinylidene fluoride membrane. After blockade with 5% non-fat dry milk in Tris buffered saline with 0.1% Tween 20 (TBST), membranes were incubated overnight at 4°C with the following primary antibodies: rabbit anti-RIP3 Ab (Abcam), anti-p-RIP3 Ab (Abcam), anti-MLKL (Abcam), anti-p-MLKL Ab (Abcam), mouse anti-NLRP3 (Adipogen), anti-caspase-1 p20 (Adipogen), rabbit anti-caspase-8 (Abcam) and anti-GAPDH Ab (Cell Signaling Technology). After washing 3 times with TBST, membranes were incubated with their corresponding secondary antibodies. The signals on the membranes were detected by a chemiluminescence analysis kit (Millipore).

Guo C., Fu R., Zhou M., Wang S., Huang Y., Hu H., Zhao J., Gaskin F., Yang N, & Fu S.M. (2019). Pathogenesis of Lupus Nephritis: RIP3 Dependent Necroptosis and NLRP3 Inflammasome Activation. Journal of autoimmunity, 103, 102286.

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Western Blot Analysis of Mouse Liver Proteins

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Fresh mouse livers were ground into powder in liquid nitrogen, and moderate protein lysis solution (RIPA : PMSF = 100 : 1) was added. After incubation on ice for 30 min, tissue debris was removed by centrifugation (15 min, 4°C). Protein concentrations were assayed by using the Bradford assay (BIO-RAD). Total protein was resolved by SDS-PAGE and then transferred to a polyvinylidene fluoride membrane (0.2 μm, Millipore). After blocking with 5% skimmed milk, membranes were probed with the appropriate antibody. Protein bands were detected with ECL reagents. The antibodies used were as follows: anti-GAPDH Ab (Cell Signaling Technology), anti-α-SMA (BM0002, BOSTER), anti-α-SMA (BM0002, BOSTER), and anti-tTG Ab (sc-20621, Santa Cruz Biotechnology).

Tang J., Huang H., Ji X., Zhu X., Li Y., She M., Yan S., Fung M, & Li Z. (2014). Involvement of IL-13 and Tissue Transglutaminase in Liver Granuloma and Fibrosis after Schistosoma japonicum Infection. Mediators of Inflammation, 2014, 753483.

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Signaling Pathways Regulation in Cellular Processes

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The primary Abs used included: anti‐TRAF6 Ab (Abcam), anti‐TRAF2 Ab (Abcam, Cambridge, MA, USA), anti‐AKT Ab (Cell Signaling Technology, Danvers, MA, USA), anti‐phospho‐AKT Ab (Thr308; Cell Signaling Technology), anti‐IκB kinase (IKK) Ab (Cell Signaling Technology), anti‐phospho‐IKKα/β Ab (Ser176/180; Cell Signaling Technology), anti‐NF‐κB p65 Ab (Cell Signaling Technology), anti‐phospho‐NF‐κB p65 Ab (Ser536; Cell Signaling Technology), anti‐IκBα Ab (Cell Signaling Technology), anti‐ phospho‐IκBα Ab (Ser32; Cell Signaling Technology), anti‐JNK Ab (Cell Signaling Technology), anti‐phospho‐JNK Ab (Thr183/Tyr185; Cell signaling), anti‐p38 Ab (Cell Signaling Technology), anti‐phospho‐p38 Ab (Thr180/Tyr182; Cell Signaling Technology), anti‐ERK Ab (Cell Signaling Technology), anti‐phospho‐ERK Ab (Thr202/Tyr204; Cell signaling), anti‐ubiquitin K63 Ab (Cell signaling), and anti‐GAPDH Ab (Cell signaling). The AKT inhibitor MK‐2206, the IKK inhibitor IKK‐16, and the proteasome inhibitor MG132 were purchased from Selleckchem (Houston, TX, USA).

Shi J., Liu Z, & Xu Q. (2019). Tumor necrosis factor receptor‐associated factor 6 contributes to malignant behavior of human cancers through promoting AKT ubiquitination and phosphorylation. Cancer Science, 110(6), 1909-1920.

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Western Blot Analysis of Key Proteins

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Western blot analysis was performed as previously described.^[⁹
^] Primary antibodies included: mouse monoclonal anti‐CD133 (W6B3C1 clone) (Miltenyi Biotec), rabbit monoclonal anti‐GAPDH Ab (Cell signaling), anti‐Akt Ab (Cell signaling), anti‐p‐Akt (Thr308) Ab (Cell signaling), anti‐PI3‐kinase p85 Ab (Millipore), and anti‐SLC1A5 antibody (Cell signaling.

Wei Y., Geng S., Si Y., Yang Y., Chen Q., Huang S., Chen X., Xu W., Liu Y, & Jiang J. (2023). The Interaction between Collagen 1 and High Mannose Type CD133 Up‐Regulates Glutamine Transporter SLC1A5 to Promote the Tumorigenesis of Glioblastoma Stem Cells. Advanced Science, 11(3), 2306715.

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