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2 protocols using transfer buffer

1

Evaluating Cytotoxicity and Apoptosis in Cancer Cells

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ALA (463-40-1) (0.914gm/ml) was purchased from TCI Chemicals (India) Pvt. Ltd. RPMI 1640 (Gibco, USA); fetal bovine serum (FBS) (Gibco-10270); trypsin (Gibco, USA); eagle balanced salt solution (EBSS)(Gibco, 2018-11); hank’s balanced salt solution (HBSS) (Himedia, TL1190); EtBr (Himedia, MB071); AO(Himedia, MB116); JC-1 assay kit (Thermo Scientific, M34152); PI (SC-3541); tamoxifen citrate (TMX) (Tammodex 20, Biochem Pharmaceuticals India); penicillin- streptomycin (Thermo Scientific, 15410-163); gentamycin (Thermo Scientific, 15710-049);3-(4, 5-Dimethyl-2-thiazolyl)-2, 5-diphenyl-2H-tetrazolium bromide (MTT) (Himedia, TC191); RNase (SRL, 58895); dimethyl sulfoxide (DMSO) (Merck, 1.16743.0521); DMBA (Sigma Aldrich, 57-97-6); ponceau S (Himedia, ML045); sodium cacodylate (Sigma Aldrich, C0250); collagenase type 4 (Himedia, TC-214); hyaluronidase (Himedia, TC331); hematoxylin (Himedia, S058); eosin (Himedia, S007); RIPA lysis buffer (Amresco, N653); protein assay kit (Amresco, M173); bovine serum albumin (BSA) (Genetix, PG-2330); transfer buffer (Genetix, GX-9411AR), trizol reagent (Sigma-T9424), cDNA synthesis kit (Genetix-K1612). Caspase 3 (SC-4263) and caspase 8 (SC-4267) assay kits were procured from Santacruz Biotechnology Inc., California, Delaware. All others chemicals were of molecular biology grade and purchased from Genetix Biotech Asia Pvt. Ltd, New Delhi.
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2

Comprehensive Molecular Characterization Protocol

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VOA (NCI-3375-85-5) was obtained from National Cancer Institute (NCI), United States as a drug sample. TMX (Tamodex20-Zydus) was purchased from the local market. DMSO (Merk), ponceauS (Himedia.ML045), hematoxyline (Himedia, So58),), Sodium Lauryl Sulfate (SLS) (Loba chemieS56971301), MNU (Sigma Aldrich,57–97-6), TEMED (Amresco-0761), acrylamide (Genetix-1443c196), Protein assay kit (Amresco, M173), bovine serum albumin (BSA) (Genetix, PG-2330), eosin (Himedia, S007), ammonium persulfate (APS) (Lobachemie-LB2282a09), Glycine (Amresco-0167-kg), TrisUlrapure (DuchefaBiochemie), RIPA lysis buffer (Amresco, N653), transfer buffer (Genetix, GX-9411AR), All other chemicals used in this study were purchased from Genetix Asia Pvt. Ltd. New Delhi, India and all the chemicals were molecular grade.
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