Chicken anti lacz

Pcdh19 antibody was raised by immunizing a rabbit with the GST-fused cytoplasmic region of Pcdh19 (Val1041-Leu1140 a.a.) and subsequently affinity-purified with the antigen. A GST-reactive population was also removed. The following antibodies were purchased: rat anti-Ctip2, chicken anti-LacZ and chicken anti-GFP (Abcam); mouse anti-Homer1 and guinea pig anti-Synapsin1/2 (Synaptic systems); rabbit anti-MAP2, mouse anti-Tau1 and guinea pig anti-Vglut2 (Millipore); rabbit anti-FLAG and mouse anti-MAP2 (Sigma-Aldrich); and mouse anti-Abi-1 and rabbit anti-RFP (MBL).

Fixation and antibody staining in imaginal discs were performed as described [70 (link)]. Fixation and antibody staining in cultured cells were performed as described [71 (link)]. Fixation and antibody staining in midguts were performed as described [37 (link)]. Primary antibodies used for the immunostaining were: mouse anti-Wdp (1:1000), chicken anti-lacZ (Abcam, 1:1000), mouse anti-Dl (DSHB, 1:50), mouse anti-Pros (DSHB, 1:200), rabbit anti-PH3 (Millipore, 1: 2000), mouse anti Brdu (DSHB, 1:200), rabbit anti Pdm1(1:1000, gift from Xiaohang Yang), mouse anti-V5 (Invitrogen, 1:3000), mouse anti-HA (Abmart, 1:500), rabbit anti-GM130 (Abcam, 1:200), rabbit anti-Rab5 (Abcam, 1:200), Gp anti-Sens (1:200), rabbit anti-Sal (1:100), and Rat anti-Ci (DSHB, 1:5). The primary antibodies were detected by fluorescent-conjugated secondary antibodies from Jackson ImmunoResearch Laboratories, Inc. The primary antibodies used for IP and western blot were: rabbit anti-V5 (Sigma, 1:1000), rabbit anti-HA (Santa Cruz, 1:1000), mouse anti-Wdp (1:500), rabbit anti-GFP (Abmart, 1:1000) and mouse anti-tubulin (Abmart, 1:1000).