Anti-Sox9 antibody was purchased from Millipore (AB5535, Billerica, MA). Anti-β-catenin and glycogen synthase kinase 3β (GSK3β) antibodies were obtained from BD Transduction Laboratories (#610153 & #610201, San Jose, CA). Anti-Oct4, Sox2 and FZD7 antibodies were purchased from Abcam (ab19857, ab97959 & ab51049, Cambridge, UK). Anti-E-cadherin, vimentin and phospho-GSK3β (Ser9) antibodies were obtained from Cell Signaling Technology (#3195, #5741 & #9336, Beverly, MA, USA). Anti-β-actin antibody was purchased from Sigma-Aldrich (A5316, St. Louis, MO, USA). Anti-CD24 (ab30350, Abcam), E-cadherin (#610181, BD Transduction Laboratories) and vimentin antibodies (M0725, Dako, Glostrup, Denmark) were used for immunofluorescent staining. Anti-CK19 (ab52625, Abcam), anti-AFP (A0008, Dako) and anti-EpCAM (M0804, Dako) were used for immunohistochemistry.
Ab51049
Manufactured by Abcam
Sourced in United Kingdom, United States
Ab51049 is a lab equipment product. It is a recombinant protein that serves as an antigen. The core function of this product is to be used as a tool in research applications.
Automatically generated - may contain errors
Lab products found in correlation
Ab52625, by Abcam (1 mentions)
Ab97959, by Abcam (1 mentions)
Anti β catenin, by BD (1 mentions)
Phospho gsk 3β ser9, by Cell Signaling Technology (1 mentions)
Ab19857, by Abcam (1 mentions)
Anti e cadherin, by Cell Signaling Technology (1 mentions)
Anti β actin antibody, by Merck Group (1 mentions)
E cadherin, by BD (1 mentions)
Anti sox9 antibody, by Merck Group (1 mentions)
Gsk3β, by BD (1 mentions)
A0008, by Agilent Technologies (1 mentions)
M0804, by Agilent Technologies (1 mentions)
Vimentin, by Cell Signaling Technology (1 mentions)
Polyvinylidene difluoride membrane, by Merck Group (1 mentions)
A5316, by Merck Group (1 mentions)
Hrp conjugated anti mouse or anti rabbit igg, by Santa Cruz Biotechnology (1 mentions)
2 protocols using ab51049
1
Immunodetection of Stem Cell Markers
2
Westerns Blotting Assay for Wnt Signaling
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Whole cell lysates were extracted using RIPA buffer supplemented with protease inhibitor cocktail. Protein lysate were separated by SDS-polyacrylaminde gel electrophoresis (SDS-PAGE) and transferred to polyvinylidene difluoride membrane (Millipore, MA, USA) for western blotting analyses. Primary antibodies against Cripto-1 (1:1000, ab108391, Abcam), total β-catenin (1:2500, 610153, BD Transduction Laboratories, San Jose, CA), Dishevelled-3 (DVL3) (1:1000, sc-8027, Santa Cruz Biotechnology), Frizzled-7 (FZD7) (1:1000, ab51049, Abcam), LRP6 (1:1000, #2560, Cell Signaling) and β-actin (1:80,000, A5316, Sigma-Aldrich, St. Louis, MO, USA) were incubated at 4 °C overnight. HRP-conjugated antirabbit or antimouse IgG were used as secondary antibodies at 1:5000 where appropriate (Santa Cruz Biotechnology, Texas, USA). The signals were visualized using the enhanced chemiluminescence method.
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