Ethanol (≥99.8%), poly(ethylene glycol) (PEG, Mn = 8000 g mol−1); poly(ethylene glycol) diacrylate (PEG-DA, Mn = 700 g mol−1); 1,6-hexanedithiol (HT); triethylamine; tetrahydrofuran; toluene; pentaerythritol tetrakis (3-mercaptopropionate) (QT); Irgacure 2959; phosphate-buffered saline without calcium and magnesium (PBS) were purchased from Sigma-Aldrich. Thioglycerol (≥98%, MT) was purchased from TCI. Tetraethoxysilane (TEOS, 98%) and calcium nitrate tetrahydrate (99.0–103%) were purchased from Alfa Aesar. For preparing the Simulated Body Fluid (SBF): sodium chloride, sodium hydrogenocarbonate, dipotassium hydrogenophosphate trihydrate and calcium chloride dihydrate were purchased from Sigma-Aldrich; magnesium chloride hexahydrate from Acros Organics; sodium disulfate, potassium chloride and tris(hydroxymethylaminomethane) from Alfa Aesar. All chemicals were used as purchased.
Phosphate buffered saline without calcium and magnesium
Manufactured by Merck Group
Sourced in United States
Phosphate-buffered saline (PBS) without calcium and magnesium is a commonly used buffer solution that maintains a physiological pH and osmolarity. It is a salt solution composed of sodium phosphate and sodium chloride in water. The absence of calcium and magnesium ions makes this solution suitable for specific applications where their presence is not desired.
Automatically generated - may contain errors
Lab products found in correlation
Calcium nitrate tetrahydrate, by Thermo Fisher Scientific (1 mentions)
Ethanol, by Merck Group (1 mentions)
Sodium chloride (nacl), by Merck Group (1 mentions)
Potassium chloride (kcl), by Thermo Fisher Scientific (1 mentions)
Magnesium chloride hexahydrate, by Thermo Fisher Scientific (1 mentions)
Toluene, by Merck Group (1 mentions)
Triethylamine, by Merck Group (1 mentions)
Tetrahydrofuran, by Merck Group (1 mentions)
Calcium chloride dihydrate, by Merck Group (1 mentions)
Tetraethoxysilane, by Thermo Fisher Scientific (1 mentions)
Irgacure 2959, by Merck Group (1 mentions)
Sodium hydrogenocarbonate, by Merck Group (1 mentions)
1 6 hexanedithiol, by Merck Group (1 mentions)
Pentaerythritol tetrakis 3 mercaptopropionate qt, by Merck Group (1 mentions)
Crystal violet, by Merck Group (1 mentions)
Glutaraldehyde, by Merck Group (1 mentions)
Cdcl2, by Merck Group (1 mentions)
Penicillin streptomycin antibiotic solution 100, by Merck Group (1 mentions)
F12k medium, by Merck Group (1 mentions)
2 protocols using phosphate buffered saline without calcium and magnesium
1
Bioinspired Tissue Engineering Scaffolds
2
Cadmium Cytokine Response Assay
Check if the same lab product or an alternative is used in the 5 most similar protocols
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The F12 K medium, penicillin/streptomycin antibiotic solution (100×), fetal bovine serum (FBS), trypsin-EDTA solution (1X), amphotericin B (1,000×), phosphate-buffered saline without calcium and magnesium, CdCl2, 25% glutaraldehyde and crystal violet were purchased from Sigma-Aldrich (St. Louis, MO, USA). The human IL-10 (cat. no. ELH-IL10–001) and human IL-1α (cat. no. ELH-IL1alpha-001) enzyme-linked immunosorbent assay (ELISA) kits were purchased from Ray Biotech, Inc. (Norcross, GA, USA).
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