Pluronic f 127 p2443

Antibodies for JNK1/2 (sc-571), phosphor-JNK1/2 (sc-6254), ERK1/2 (sc-153), phosphor-ERK1/2 (sc-7383), p38 (sc-535), Tubulin (sc-69969), and VEGF (sc-152) were purchased from Santa Cruz Biotechnology (Dallas, TX, USA). Antibodies for goat anti-rabbit IgG Alexa fluor 488 (A-11034) and goat anti-mouse IgG Alexa fluor 488 (A-11029) secondary antibody were purchased from Invitrogen (Carlsbad, CA, USA). Antibodies for phospho-p38 (#9216), Rabbit-HRP (#7074) and Mouse-HRP (#7076) were purchased from Cell Signaling (Danvers, MA, USA). Recombinant human visfatin (10990-H20B) was purchased from Sino Biological (Beijing, China). Pluronic^®F-127 (P2443) for the preparation of hydrogel (22%) to delivered EGF or visfatin was purchased from Sigma Aldrich (Kenilworth, Merck, Germany).

The stock solution containing monoclonal antibodies type IgG1 at a concentration of 19.5 mg/mL was provided by Lek Pharmaceuticals d.d. (member of Novartis), and the other reagents were purchased from Sigma (St. Louis, MI, USA): urea (51456), Pluronic F 127 (P2443), HEPES (H3375), BSA (A3294), and PBS (P4417). The mAb particles were prepared by stirring the mAb stock solution on a magnetic stirrer for 2 h @400 RPM at room temperature. The pH of the stock solutions was 7. The isoelectric point of mAb was approximately 8.

Anti-TRPV1 (1:100, ab6166), anti-LC3 (1:1000, ab192890), anti-P62 (1:1000, ab109012), anti-LAMP2 (1:100, ab13524), anti-H2 Db/H2-D1(1:100, ab25244), and Alexa Fluor 568 (1:500), Alexa Fluor 488 (1:500) secondary antibody were purchased from Abcam. Anti-CGRP (1:100, sc-57053) was purchased from Santa cruze. Anti-mouse CD45-PE-cy7 (103114), anti-mouse CD11c-APC-cy7 (117323), anti-mouse CD3-APC-cy7 (100221), anti-mouse CD8a-APC (100711), anti-mouse CD16/32-TruStain FcX™ (101320), anti-mouse CD86-APC (105011) antibodies were purchased from BioLegend. APC anti-mouse H-2 (E-AB-F1216E) and Anti-mouse CD80-FITC (E-AB-F0992C) were purchased from Elabscience. HRP-conjugated anti-rabbit antibody (W4011) and HRP-conjugated anti-mouse antibody (W4021) were purchased from Promega. Enhanced chemiluminescence (ECL) kit was purchased from Biological Industries. One Step TUNEL Apoptosis Assay Kit (Green Fluorescence) (C1088) and Ad-GFP-LC3B (C3006) were purchased from Beyotime. Lyso-Tracker Red (L12492) was purchased from Invitrogen. Imiquimod (HY-B0180A) was purchased from MCE. Indocyanine Green (3599-32-4) was purchased from Aladdin. Morphine (161007-2) was purchased from NORTHEAST PHARM. Ropivacaine (R413090) was purchased from Mackline. Pluronic F127 (P2443) was purchased from Sigma.