Liver tissue was fixed in 10% buffered formalin and embedded in paraffin sections. Four millimeter sections were cut and mounted on glass slides. After dehydration, the sections were stained with hematoxylin and eosin. An IX71 system biological microscope (Olympus) was used for morphological observation.
Ix71 system biological microscope
Manufactured by Olympus
The IX71 system biological microscope is a high-performance microscope designed for use in life science research and applications. It features a modular design that allows for customization and integration of various accessories and imaging modules. The core function of the IX71 is to provide a stable and reliable platform for magnified observation and analysis of biological samples.
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Lab products found in correlation
2 protocols using ix71 system biological microscope
1
Histological Analysis of Liver Tissue
2
Intracellular ROS Measurement Protocol
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The level of intracellular ROS generation was assessed using the ELISA kit (Shanghai Yanjing Biological Research Technology Co, LTD, China) according to manufacturer's instruction. Furthermore, fluorescence microscopy was used to confirm the results of the microplate ROS assay. Briefly, after the indicated treatments, cells were washed twice with phosphate-buffered saline and then incubated with serum-free DMEM containing 10μmol/l dichlorofluorescein diacetate (DCFH-DA) at 37˚C for 30 min. After that, cells were washed with PBS three times in order to eliminate the residual DCFH-DA. Cells from each group were analyzed by measuring the excitation and emission spectrum at 488 and 525 nm, respectively. The fluorescence was visualized using a fluorescence microscope coupled with an image analysis system Olympus IX71 system biological microscope.
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