Adenosine 5 monophosphate disodium salt 5 amp

Example 1

Ethidium bromide (EB), sodium N,N-dimethyldithiocarbamate hydrate (DMDT), and sodium N,N-diethyldithiocarbamate hydrate (DEDT) were purchased from Sigma-Aldrich. Tris-(hydroxymethyl)aminomethane (Tris buffer) (Merck), adenosine-5′-monophosphate disodium salt (5′-AMP), cytidine-5′-monophosphate disodium salt hydrate (5′-CMP), guanosine-5′-monophosphate disodium salt (5′-GMP), thymine-5′-monophosphate (5′-TMP), and CH₃OH were obtained from Fluka Chemicals Co. and were stored at −20° C. Disodium salt of CT-DNA (calf thymus DNA) was purchased from Sigma Chem. Co. and was stored at 4° C. All reagents as well as solvents were used as received. Human breast cancer cell lines, MDA-MB231 and MCF7, were provided by American Type Culture Collection (ATCC). Cells were cultured in Dulbecco's Modified Eagle Medium (DMEM) supplemented with 10% fetal calf serum (FCS), penicillin (100 kU L⁻¹) and streptomycin (0.1 g L⁻¹) at 37° C. in a 5% CO₂-95% air atmosphere. MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide), a yellow tetrazole, was purchased from Sigma Chemical Co, St. Louis, Mo., USA.

Example 1

Ethidium bromide (EB), sodium N,N-dimethyldithiocarbamate hydrate (DMDT), and sodium N,N-diethyldithiocarbamate hydrate (DEDT) were purchased from Sigma-Aldrich. Tris-(hydroxymethyl)aminomethane (Tris buffer) (Merck), adenosine-5′-monophosphate disodium salt (5′-AMP), cytidine-5′-monophosphate disodium salt hydrate (5′-CMP), guanosine-5′-monophosphate disodium salt (5′-GMP), thymine-5′-monophosphate (5′-TMP), and CH₃OH were obtained from Fluka Chemicals Co. and were stored at −20° C. Disodium salt of CT-DNA (calf thymus DNA) was purchased from Sigma Chem. Co. and was stored at 4° C. All reagents as well as solvents were used as received. Human breast cancer cell lines, MDA-MB231 and MCF7, were provided by American Type Culture Collection (ATCC). Cells were cultured in Dulbecco's Modified Eagle Medium (DMEM) supplemented with 10% fetal calf serum (FCS), penicillin (100 kU L⁻¹) and streptomycin (0.1 g L⁻¹) at 37° C. in a 5% CO₂-95% air atmosphere. MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide), a yellow tetrazole, was purchased from Sigma Chemical Co, St. Louis, Mo., USA.

Example 1

Ethidium bromide (EB), sodium N,N-dimethyldithiocarbamate hydrate (DMDT), and sodium N,N-diethyldithiocarbamate hydrate (DEDT) were purchased from Sigma-Aldrich. Tris-(hydroxymethyl)aminomethane (Tris buffer) (Merck), adenosine-5′-monophosphate disodium salt (5′-AMP), cytidine-5′-monophosphate disodium salt hydrate (5′-CMP), guanosine-5′-monophosphate disodium salt (5′-GMP), thymine-5′-monophosphate (5′-TMP), and CH₃OH were obtained from Fluka Chemicals Co. and were stored at −20° C. Disodium salt of CT-DNA (calf thymus DNA) was purchased from Sigma Chem. Co. and was stored at 4° C. All reagents as well as solvents were used as received. Human breast cancer cell lines, MDA-MB231 and MCF7, were provided by American Type Culture Collection (ATCC). Cells were cultured in Dulbecco's Modified Eagle Medium (DMEM) supplemented with 10% fetal calf serum (FCS), penicillin (100 kU L⁻¹) and streptomycin (0.1 g L⁻¹) at 37° C. in a 5% CO₂—95% air atmosphere. MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide), a yellow tetrazole, was purchased from Sigma Chemical Co, St. Louis, Mo., USA.