Carvedilol was kindly supplied from Global Napi Pharmaceuticals, 6th of October, Egypt. BSA was purchased from Sigma-Aldrich, St. Louis, MO, USA. Acetone was purchased from Scharlau, Barcelona, Spain. Triethyl amine and glutaraldehyde (25%) were purchased from Loba Chemie, Mumbai, India. Disodium hydrogen orthophosphate and potassium dihydrogen orthophosphate were purchased from El-Nasr Pharmaceutical Chemicals, Cairo, Egypt. Acetonitrile and methanol (HPLC grade) were purchased from Fisher Scientific, Loughborough, UK. The other chemicals were of analytical grade.
Glutaraldehyde
Manufactured by Loba Chemie
Sourced in India
Glutaraldehyde is a colorless, pungent-smelling chemical compound. It is a five-carbon dialdehyde that is commonly used as a fixative in electron microscopy and as a disinfectant in medical and laboratory settings.
Automatically generated - may contain errors
Lab products found in correlation
Triethylamine, by Loba Chemie (1 mentions)
Bovine serum albumin (bsa), by Merck Group (1 mentions)
Methanol, by Thermo Fisher Scientific (1 mentions)
Acetonitrile, by Thermo Fisher Scientific (1 mentions)
Acetone, by Scharlab (1 mentions)
Fecl3 6h2o, by Loba Chemie (1 mentions)
Toluene, by Merck Group (1 mentions)
Casein, by Merck Group (1 mentions)
Ammonium persulphate aps, by Merck Group (1 mentions)
N isopropylacrylamide, by Thermo Fisher Scientific (1 mentions)
Chitosan, by Merck Group (1 mentions)
N n n n tetramethylethylenediamine temed, by Loba Chemie (1 mentions)
Sodium hydroxide (naoh), by Loba Chemie (1 mentions)
Gelatin, by Loba Chemie (1 mentions)
Sodium borohydride, by Loba Chemie (1 mentions)
Ethidium bromide, by Loba Chemie (1 mentions)
Silver nitrate, by Loba Chemie (1 mentions)
Rhodamine 123, by Merck Group (1 mentions)
Dulbecco s phosphate buffered saline dpbs, by Merck Group (1 mentions)
Mcf 7 cell line, by HiMedia (1 mentions)
7 protocols using glutaraldehyde
1
Carvedilol Analytical Method Development
2
Casein-based Iron Oxide Nanocomposite
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Casein was purchased from Merck, Mumbai, India and used without any pretreatment. FeCl2. H2O, FeCl3.6H2O, glutaraldehyde (used as a crosslinker) were obtained from Loba Chemie, Mumbai, India. Toluene was obtained from Sigma Aldrich Co., USA, and used for preparing oil phase. Other chemicals like acetone, NaOH etc. were of analytical reagent (AR) grade and double distilled water was used throughout the experiments.
3
Synthesis of Poly(N-isopropylacrylamide)-Chitosan Cryogels
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Poly(N-isopropylacrylamide)-chitosan cryogels were synthesized using a method previously described20 (link). A solution of 2% chitosan (low viscosity, Sigma, USA) was prepared in 5 mL of 0.1 M acetic acid. Solutions of N-isopropylacrylamide (Acros Organic, NJ, USA) and methyl-bisacrylamide (MBAAm) (Sisco Research Labs, Mumbai, India) (NiPAAm:MBAAm ratio 3:1) were prepared in 4 mL degassed water. Both the solutions were mixed and ammonium persulphate (APS) (0.2% w/v) (Merck, Mumbai, India) added. Further glutaraldehyde (chitosan:glutaraldehyde ratio 15:1) (Loba Chemie, Mumbai, India) and N,N,N’,N’-tetramethylethylenediamine (TEMED) (Loba Chemie, Mumbai, India) (0.15% w/v) were added and mixed thoroughly. The solutions were immediately poured in appropriate moulds (2.5 mL syringes or non-treated 6 well plates) and frozen at −12 °C for 16 h. Post incubation, cryogels were thawed at room temperature and washed with distilled water followed by air drying.
4
Dentin Treatment Efficacy Comparison
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All teeth were randomly divided according to the dentin treatment into five groups as follows: control group (with no treatment done), 5% glutaraldehyde (Loba Chemie Pvt Ltd., Mumbai, India), 6.5% grape seed extract (Herbal Bio Solutions, Delhi, India), 0.5% hesperidin (Herbal Bio Solutions, Delhi, India), and CPP-ACP (GC Corporation, Alsip, IL, USA).
5
Composite Hydrogel Synthesis Protocol
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Cellulose, gelatin, silver nitrate (AgNO3), acrylic acid (AA), APS, glutaraldehyde, sodium borohydride, sodium hydroxide, ethidium bromide (EtBr), and eosin were procured from Loba Chemie and were of analytical grade.
6
Silk Fibroin-Based Biomaterials for Cancer Therapeutics
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Silk cocoons
of B. mori (silkworm) were procured
from Central Silk Board, Dehradun, Uttarakhand, India. Poly(vinyl
alcohol) (PVA) (HiMedia, cat. no. GRM6170), sodium carbonate (HiMedia,
cat.no. GRM851), sodium bicarbonate (HiMedia, cat. no. MB045), lithium
bromide (SRL, cat. no. 31665), dialysis membrane (MWCO 12 kDa, HiMedia,
cat. no. LA401), ethanol (CSS, cat.no. 1170), glutaraldehyde (25%,
for synthesis, LOBA CHEMIE Pvt. Ltd., cat no. 03965), and formic acid,
98% AR (LOBA CHEMIE Pvt. Ltd., cat no. 00153) were purchased and used
as received. MCF-7 cell line (breast cancer cell line) was obtained
from NCCS, Pune, and cultured in Dulbecco’s modified eagle
medium (DMEM) (HiMedia, cat.no. AT007) containing 10% fetal bovine
serum (FBS, Gibco, cat. no. 10270–106) and 1% penicillin–streptomycin
(Gibco, cat. no. 10378-016). For cell culture experiments, Dulbecco’s
phosphate-buffered saline (DPBS, Ca2+- and Mg2+-free, Sigma-Aldrich, cat.no. D5652), trypsin-ethylenediaminetetraacetic
acid (EDTA) (0.25%, Sigma-Aldrich, cat. no. 25200-070), MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium
bromide, Amresco, cat.no. 0793), Hoechst 33342 (Sigma-Aldrich, cat.
no. 14533), and Rhodamine 123 (Sigma-Aldrich, cat. no. R8004) were
used.
of B. mori (silkworm) were procured
from Central Silk Board, Dehradun, Uttarakhand, India. Poly(vinyl
alcohol) (PVA) (HiMedia, cat. no. GRM6170), sodium carbonate (HiMedia,
cat.no. GRM851), sodium bicarbonate (HiMedia, cat. no. MB045), lithium
bromide (SRL, cat. no. 31665), dialysis membrane (MWCO 12 kDa, HiMedia,
cat. no. LA401), ethanol (CSS, cat.no. 1170), glutaraldehyde (25%,
for synthesis, LOBA CHEMIE Pvt. Ltd., cat no. 03965), and formic acid,
98% AR (LOBA CHEMIE Pvt. Ltd., cat no. 00153) were purchased and used
as received. MCF-7 cell line (breast cancer cell line) was obtained
from NCCS, Pune, and cultured in Dulbecco’s modified eagle
medium (DMEM) (HiMedia, cat.no. AT007) containing 10% fetal bovine
serum (FBS, Gibco, cat. no. 10270–106) and 1% penicillin–streptomycin
(Gibco, cat. no. 10378-016). For cell culture experiments, Dulbecco’s
phosphate-buffered saline (DPBS, Ca2+- and Mg2+-free, Sigma-Aldrich, cat.no. D5652), trypsin-ethylenediaminetetraacetic
acid (EDTA) (0.25%, Sigma-Aldrich, cat. no. 25200-070), MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium
bromide, Amresco, cat.no. 0793), Hoechst 33342 (Sigma-Aldrich, cat.
no. 14533), and Rhodamine 123 (Sigma-Aldrich, cat. no. R8004) were
used.
7
Gelatin-based Hydrogel for Cell Culture
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Gelatin (from cold water fish skin), HEMA, 97%, poly(ethylene glycol) diacrylate (PEGDA), Dulbecco’s modified Eagle’s medium (DMEM), 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT, 98%), TE, 4′-6-diamidino-2-phenylindole (DAPI), and β-endothelial cell growth factor (human) were purchased from Sigma Chemical Co. (St. Louis, MO, USA). Agarose (low EEO) and HEPES were obtained from Qualigens Fine Chemicals (Mumbai, India). Glutaraldehyde (25% in aqueous solution) and DMSO were purchased from Loba Chemie (Mumbai, India). Fetal bovine serum (FBS) and streptomycin-penicillin antibiotic solutions were bought from HyClone (Utah, USA). N,N,N′,N′-tetramethyleneethylenediamine (TEMED) was obtained from Bio-Rad Labs (Hercules, CA, United States) and ammonium persulfate (APS) was from Merck Limited (Mumbai, India). All other chemicals used were of analytical grade.
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