Amylase

Manufactured by MP Biomedicals

Amylase is an enzyme that catalyzes the breakdown of starch into smaller carbohydrate molecules. It is commonly used in various laboratory applications to hydrolyze starch and measure its degradation.

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Lab products found in correlation

Pectinase, by Merck Group (2 mentions) Zymolyase 20t, by AMS Biotechnology (1 mentions) Dnase 1, by Zymo Research (1 mentions) Pepsin, by Merck Group (1 mentions) Zymolyase 100t, by US Biological (1 mentions)

2 protocols using amylase

Enzymatic Biomolecule Extraction and Processing

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Enzymes used in this study were sourced from various companies; cellulase (Aspergillus niger) MP Biomedicals, catalog number 150583; amylase (Bacillus sp.), MP Biomedicals, catalog number 100447; pectinase (Rhizopus sp.), Sigma-Aldrich, catalog number P2401; zymolyase 20T (Arthrobacter luteus), Amsbio, catalog number 120491–1; zymolyase 100T (Arthrobacter luteus), United States Biological, catalog number Z1004; pepsin (Porcine gastric mucosa), Sigma-Aldrich, catalog number P7000; DNase I, Zymo Research, catalog number E1010. All enzymes were suspended in phosphate-buffered saline at pH 7.4 to the desired concentration.

Ellis J.R, & Rowley P.A. (2023). An apparent lack of synergy between degradative enzymes against Staphylococcus aureus biofilms. bioRxiv.

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Biofilm Experiments with S. aureus

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The bacterial strain S. aureus SA31 was used for all biofilm experiments. P. pastoris strains were obtained from the Fungal Genome Stock Center (FGSC) and were maintained on yeast extract, peptone, and dextrose (YPD) media. All recombinant enzymes from the collection of Bauer et al. are listed in Supplementary Table S1 [26 (link)]. Commercial amylase and cellulase were sourced from MP Biomedicals (catalog numbers 02100447-CF and 02150583-CF, respectively). Commercial pectinase was sourced from Sigma Aldrich (catalog number P2401). All commercial enzymes were dissolved in phosphate buffered saline at a pH 7.0 (PBS).

Ellis J.R., Bull J.J, & Rowley P.A. (2023). Fungal Glycoside Hydrolases Display Unique Specificities for Polysaccharides and Staphylococcus aureus Biofilms. Microorganisms, 11(2), 293.

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