Cortactin

KM12C colon carcinoma cells (Morikawa et al., 1988 (link)) were cultured in MEM (Cellgro) supplemented with 10% fetal bovine serum (FBS; Cellgro), non-essential amino acids, MEM vitamin solution and sodium pyruvate, as described previously (Espejo et al., 2010 (link)). HCT116 colon carcinoma cells were maintained in McCoy's 5A (Cellgro) medium supplemented with 10% FBS. HEK 293T cells were maintained in DMEM (Sigma) containing 10% FBS. Epidermal growth factor was purchased from Sigma. Rat-tail collagen type I was purchased from BD Biosciences. Monoclonal antibodies to p120, E-cadherin, α-catenin, p190 Rho GAP, cortactin, Living Colors™ anti-YFP antibody, JL-8, and Rac1 were purchased from BD Biosciences. Anti-E-cadherin antibody, DECMA-1, was purchased from Abcam. Mouse-specific p120 antibody, 8D11, was a generous gift from A. Reynolds (Vanderbilt University, Nashville, TN). Polyclonal rabbit anti-p120 antibody was obtained from Santa Cruz and rabbit antiserum to β-catenin was purchased from Sigma. Rabbit anti-PTPN12 (PTP-PEST) was purchased from Sigma. Anti-phosphotyrosine antibody, 4G10, was purchased from Millipore. VAV2 antibodies were used as described previously (Liu and Burridge, 2000 (link)) or purchased from Cell Signaling. Anti-phospho-Y172-VAV2 antibody was purchased from Abcam. RhoA antibody was purchased from Santa Cruz.