Kinases inhibitors library consisting of 93 chemical compounds was purchased from Selleck (Houston, TX, USA). The compounds were dissolved in DMSO as 30 mM stock solution and kept at −20°C until use. KYSE-150 cells were seeded into 384-wells plate (5×103 cells per well) and cultured until adherent growth. After 4-hr incubation with 30 μM kinase inhibitors, the cells were treated with 6-Gy radiation, and the expression of γ-H2AX, a marker of DNA double-strand breaks (DSBs) was detected 24 h later. We chose the kinase inhibitors which can induce high γ-H2AX expression in KYSE-150 cells following irradiation for further studies.
Kinases inhibitors library
Manufactured by Selleck Chemicals
Sourced in United States
Kinases inhibitors library is a collection of chemical compounds designed to selectively inhibit the activity of various kinase enzymes. Kinases play a crucial role in cellular signaling pathways and are involved in a wide range of biological processes. This library provides researchers with a diverse set of kinase inhibitors to aid in the investigation of kinase function and the development of potential therapeutic agents.
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Lab products found in correlation
2 protocols using kinases inhibitors library
1
Kinase Inhibitor Screening for Radiation Sensitization
2
Kinase Inhibitors Impact on Radiation-Induced DNA Damage
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Kinases inhibitors library shown in Supplementary Table S5 was purchased from Selleck (Houston, TX, USA). The compounds were dissolved in DMSO to make 30 mM of stock solution and kept in −20 °C until use. The tested cells were seeded into 384-wells plate (5×103 per well) and cultured for adherent growth. After 4 h of incubation with 30 μM of various kinases inhibitors, cells were treated with 6 Gy of radiation, and detection of γ-H2AX expression was performed 30 min later as described above.
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