D gluconolactone

Manufactured by Merck Group

Sourced in Germany, United States

D-gluconolactone is a chemical compound that is commonly used in laboratory settings. It is a cyclic ester of gluconic acid and is a white crystalline solid. D-gluconolactone is a versatile compound that can be used in various applications, such as in the production of gluconic acid and as a buffer in biochemical assays.

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Gluconolactone (6 citations)

4 protocols using d gluconolactone

Colorimetric Assay for β-Glucosidase Activity

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p-nitrophenyl-β-D-glucopyranoside (PNPG), pure sodium taurocholate (6 mg/ml), Triton X-100, glucose, and D-gluconolactone (D-glucono-1,5-lactone), were obtained from Sigma-Aldrich (Germany). Additionally, we used pure sodium acetate buffer (50 mM; 0.4355% w/v CH₃COO Na/, 0.1089% w/v CH₃COOH, pH 5), and lysis buffer (155 mmol/L NH₄Cl; 10 mmol/L NaHCO₃; 0.1 mmol/L EDTA) obtained from Semikem (Bosnia and Herzegovina). Blood samples were acquired using the guidelines and approval of the University of Burch Research Ethics Committee (30052016). Written informed consent was obtained from two healthy adult male donors. Whole EDTA blood (10 ml) was collected aseptically.

Karatas M., Dogan S., Spahiu E., Ašić A., Bešić L, & Turan Y. (2020). Enzyme kinetics and inhibition parameters of human leukocyte glucosylceramidase. Heliyon, 6(11), e05191.

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Methylglyoxal-Induced Glycation Assay

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Aminoguanidine hydrochloride (AG), MGO (40% aqueous solution), bovine serum albumin (essentially fatty acid-free), acetic anhydride, pyridine, magnesium sulfate (MgSO₄), d-gluconolactone, N-acetyl-glycyl-lysine methyl ester acetate salt (G.K. peptide), d-ribose, d-glucose, sodium azide (NaN₃), sodium bicarbonate, methanol (MeOH), anhydrous acetone, prenyl bromide, potassium carbonate (K₂CO₃), Griess reagent system, and CS were purchased from Sigma-Aldrich (St. Louis, MO, USA). The 3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium, inner salt (MTS) assay kit was purchased from Promega (Promega Co., Madison, WI, USA). All solvents and other reagents used in this study, unless otherwise specified, were analytical grade and purchased from Sigma-Aldrich (St. Louis, MO, USA).

Hwang S.H., Kim H.Y., Zuo G., Wang Z., Lee J.Y, & Lim S.S. (2018). Anti-glycation, Carbonyl Trapping and Anti-inflammatory Activities of Chrysin Derivatives. Molecules : A Journal of Synthetic Chemistry and Natural Product Chemistry, 23(7), 1752.

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VOC Detection Protocol Reagents

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Chemicals used for the detection of VOCs including D-gluconolactone and 4-methyl-2-pentanol were purchased in Sigma-Aldrich (SHH, CHN), and PVPP, NaCl used for extraction of VOCs were purchased in Sangon Biotech (SHH, CHN). Moreover, TB Green^® Fast qPCR Mix used for qRT-PCR was purchased in TaKaRa (Dalian, China).

Li J., Quan Y., Wu Z., Han J., Zhang Y., Javed H.U., Ma C., Jiu S., Zhang C., Wang L, & Wang S. (2023). EBR and JA regulate aroma substance biosynthesis in ‘Ruidu Hongyu’ grapevine berries by transcriptome and metabolite combined analysis. Frontiers in Plant Science, 14, 1185049.

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Optimizing Cheese Curd pH Adjustment

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Each batch of SM and concentrates was divided into 2 batches: one was kept at the initial pH (pHi), whereas the second was adjusted to pH 6.50, which corresponds to the usual renneting pH for cheddar cheese (Ong et al., 2012) . For the pH-adjusted batches, the pH was adjusted by adding d-gluconolactone (Sigma-Aldrich, St. Louis, MO) or 1 M NaOH (Thermo Fisher Scientific, Waltham, MA) to reach approximately pH 6.45, and samples were equilibrated overnight at 4°C. Before analyses or coagulation tests, the pH was readjusted, if necessary, with 1 M NaOH to reach 6.50 and samples were warmed to 32°C and held for 1 h at this temperature.

Lauzin A., Bérubé A., Britten M, & Pouliot Y. (2019). Effect of pH adjustment on the composition and rennet-gelation properties of milk concentrates made from ultrafiltration and reverse osmosis. Journal of dairy science, 102(5).

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