Mini amp dna kit

Manufactured by Qiagen

The Mini-Amp DNA kit is a laboratory equipment designed for the rapid and efficient extraction of DNA from a variety of sample types. It utilizes a straightforward protocol to isolate high-quality DNA suitable for downstream applications such as PCR, sequencing, and other molecular biology techniques.

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Lab products found in correlation

Ficoll paque plus, by GE Healthcare (2 mentions) Ez 96 dna methylation gold kit, by Zymo Research (2 mentions) Humanmethylation450 beadchip, by Illumina (2 mentions) Humanmethylation450 array, by Illumina (1 mentions)

Close spelling variants of this product

Mini Kits (17 citations) Mini Amp kit (7 citations) DNA kits (7 citations) QIA amp DNA tissue & blood mini kit (2 citations)

3 protocols using mini amp dna kit

DNA Methylation Profiling of Colorectal Cancer

Cited 1 time

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The set of patients used in the current study consist of a subset of a larger collection of 2,203 samples from 2,101 unique donors (1,103 cases of CRC and 998 controls) that were profiled on the HumanMethylation450 array from Illumina^{15 (link)}. Lymphocyte pellets were extracted from whole blood using Ficoll-Paque PLUS (GE Healthcare). DNA was extracted from lymphocytes using phenol–chloroform or the Qiagen Mini-Amp DNA kit, except for 99 samples (90 cases, 9 controls), for which DNA was extracted from lymphoblastoid cell lines. We used 15 μl of DNA from all samples at concentrations averaging 90 ng/μl (20 ng/μl s.e.). DNA samples were bisulfite-converted using the EZ-96 DNA Methylation-Gold Kit (Zymo Research, Orange, CA); 4 μl of bisulfite-treated DNA was then analysed on the HumanMethylation450 BeadChip from Illumina according to the manufacturer’s protocol.
The OFCCR methylation data was deposited in dbGaP under the accession number phs000779.v1.p1.

Lemire M., Zaidi S.H., Zanke B.W., Gallinger S., Hudson T.J, & Cleary S.P. (2015). The effect of 5-fluorouracil/leucovorin chemotherapy on CpG methylation, or the confounding role of leukocyte heterogeneity: an illustration. Genomics, 106(6), 340-347.

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DNA Methylation Profiling of Colorectal Cancer

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We profiled 2,203 samples from 2,101 unique donors (1,103 cases of colorectal cancer and 998 controls). Lymphocyte pellets were extracted from whole blood using Ficoll-Paque PLUS (GE Healthcare). DNA was extracted from lymphocytes using phenol–chloroform or the Qiagen Mini-Amp DNA kit, except for 99 samples (90 cases, 9 controls), for which DNA was extracted from lymphoblastoid cell lines. We used 15 μl of DNA from all samples at concentrations averaging 90 ng μl⁻¹ (20 ng μl⁻¹ s.e.). DNA samples were bisulfite-converted using the EZ-96 DNA Methylation-Gold Kit (Zymo Research, Orange, CA); 4 μl of bisulfite-treated DNA was then analysed on the HumanMethylation450 BeadChip from Illumina according to the manufacturer’s protocol. Except for the case/control status, the plating of the DNA samples was not based on any specific characteristics that they might share (for example, gender and age), effectively randomizing these factors on the arrays. Most cases and most controls were plated and processed separately, except for 125 cases that were matched to 125 controls on 125 rows of 36 arrays; for these samples, batch and position effects are controlled for by design.

Lemire M., Zaidi S.H., Ban M., Ge B., Aïssi D., Germain M., Kassam I., Wang M., Zanke B.W., Gagnon F., Morange P.E., Trégouët D.A., Wells P.S., Sawcer S., Gallinger S., Pastinen T, & Hudson T.J. (2015). Long-range epigenetic regulation is conferred by genetic variation located at thousands of independent loci. Nature Communications, 6, 6326.

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Profiling HPV in Oral Cancer Samples

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The archival samples from the cases reported to the Dental College within the duration of 2 years were selected. The samples were grouped into three groups, Group I-patients with oral squamous cell carcinoma (OSCC), Group II-patients with dysplasia (oral epithelial dysplasia), and Group III-control group included patients who had reported for impaction, the surrounding tissue was used in the study. The ethical clearance was obtained from the college ethical board. The samples were fixed in 4% formalin and paraffin embedded. A section of 40 μ thick was utilized for DNA extraction for each specimen. Care was taken to prevent contamination during tissue sectioning. New blades were used for each block. The area was cleaned with xylene between each block.
DNA was extracted with the Qiagen Mini-AMP DNA kit. The proteinase K incubation was done overnight at 56°C after discussion with the technical team to increase the yield from paraffin sections. The samples with adequate quantity of DNA were then amplified with a house keeping gene glutamate dehydrogenase (GluDH) the primer was selected such that the final amplicon size was small. The samples which were positive for the PCR amplification of GluDH was amplified for the E7 and E1 genes of HPV 16 and 18, respectively. The PCR amplifications were carried out in separate labs to prevent contamination [Table 1].

Dhanapal R., Ranganathan K., Kondaiah P., Devi R.U., Joshua E, & Saraswathi T.R. (2015). High-risk human papilloma virus in archival tissues of oral pathosis and normal oral mucosa. Contemporary Clinical Dentistry, 6(2), 148-152.

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