CD11bCre transgenic mice were provided by G. Kollias (Biomedical Sciences Research Centre “Alexander Fleming,” Vari, Greece; Boillée et al., 2006 (link)). Tie2eCre transgenic mice were a gift of X.-Y. Fu (National University of Singapore, Singapore; Kano et al., 2003 (link)). MyD88 flox mice were provided by A. DeFranco (University of California, San Francisco, CA; Hou et al., 2008 (link)). All these transgenic mice used backcrossed 10 times to C57/BL6 background. ApoE−/− mice in the C57/BL6 background were purchased from The Jackson Laboratory. Mice were housed in a specific pathogen–free facility (12-h light/dark cycle) and were fed either standard rodent chow or a Western style HFD (Harlan Teklad 88137; Tordjman et al., 2001 (link); Chen et al., 2005 (link); Alkhouri et al., 2010 (link); Fernández-Real et al., 2011 (link)). Animals were fasted overnight and blood samples were taken for assessment of insulin (Crystal Chem), FFA(Cayman), cholesterol (Sigma-Aldrich), triglycerides (Sigma-Aldrich) and IL-1β, IL-6, TNF, CXCL1 (R&D Systems) levels according to manufacturer’s instructions. All experimental procedures were performed with the approval of the Institutional Animal Care and Use Committee of Cleveland Clinic (Cleveland, OH).
Triglyceride
Manufactured by Merck Group
Sourced in United States, Germany
Triglycerides are a type of lab equipment used for the measurement and analysis of triglyceride levels in biological samples. Triglycerides are the main form of fat stored in the body and play a crucial role in energy metabolism. This lab equipment is designed to accurately quantify the concentration of triglycerides in various specimens, such as blood or tissue samples, providing important information for clinical and research applications.
Automatically generated - may contain errors
Lab products found in correlation
Cholesterol, by Merck Group (5 mentions)
Glucose, by Merck Group (3 mentions)
Urea, by Merck Group (3 mentions)
Cobas pentra c400 analyzer, by Horiba (2 mentions)
Non esterified fatty acid, by Fujifilm (2 mentions)
Omegawax 250 column, by Merck Group (2 mentions)
Minispec lf90ii, by Bruker (2 mentions)
Hemoglobin, by Merck Group (2 mentions)
Ammonium acetate, by Merck Group (2 mentions)
Pyridine, by Merck Group (2 mentions)
Leptin, by Merck Group (2 mentions)
Hydrochloric acid (hcl), by Merck Group (2 mentions)
Chloroform, by Merck Group (2 mentions)
Formic acid, by Merck Group (2 mentions)
Insulin, by Crystal Chem (2 mentions)
Fetal bovine serum (fbs), by Thermo Fisher Scientific (2 mentions)
Uric acid, by Merck Group (2 mentions)
Trichloroacetic acid, by Merck Group (2 mentions)
Diglyceride, by Merck Group (2 mentions)
Potassium chloride (kcl), by Merck Group (2 mentions)
31 protocols using triglyceride
1
Transgenic Mice for Metabolic Studies
2
Quantifying Lipid Content via Sulfo-Phospho-Vanillin
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The lipid content was evaluated by the Sulfo-Phospho-vanillin assay. Briefly, samples were incubated with 95% sulfuric acid at 95 °C for 20 min, quickly cooled, and evaluated at 535 nm. Afterward, a solution of 0.2 mg/mL vanillin (#V1104, Sigma-Aldrich, St. Louis, MO, USA) in 17% aqueous phosphoric acid was added to the samples, incubated for 10 min in the dark, and reevaluated at 535 nm. A mix of triglycerides (#17810, Sigma-Aldrich, St. Louis, MO, USA) was used to obtain a standard curve [12 (link)].
3
Plasma and Serum Biomarker Quantification
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Blood collected in EDTA-coated tubes was immediately stored on ice, centrifuged, and plasma was stored at −80 °C until analyses Blood collected in serum tubes was stored at room temperature for at least 30 min to allow coagulation, followed by centrifugation and storage at −80 °C until analyses. Glucose (Horiba, Montpellier, France), FFA (WAKO, Neuss, Germany), and triglycerides (Sigma, St. Louis, MI, USA) levels were measured colorimetrically using a Cobas Pentra C400 analyzer (Horiba, Montpellier, France). All samples from one participant were analyzed within the same run.
4
Acute Exercise Metabolic Responses in Mice
5
Quantitative Liver Lipid Analysis
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Plasma concentrations of triglycerides (Sigma) and non-esterified fatty acid (Wako Diagnostics) were measured using commercial assay kits. Liver triglyceride was extracted and measured as previously described 58 (link). Body fat and lean mass were measured using an NMR analyzer (Minispec LF90II, Bruker Optics). For liver lipid analysis, lipids were extracted by the method of Bligh-Dyer in the presence of an internal standard (T21:0 TAG, 10 nmol/mg protein) and separated on silica gel 60-Å plates that were developed with a nonpolar acidic mobile phase (70:30:1, v/v/v, hexane/ethyl ether/acetic acid). Briefly, spots corresponding to TAG were visualized with 0.01% rhodamine 6G and identified with TAG standard. The bands were scraped, extracted, and treated with acidic methanol. Quantitative GC analysis of resulting FA methyl esters was conducted (Hewlett-Packard 5890 GC; Hewlett-Packard, Palo Alto, CA, USA) with a 30-m×0.32 mm Omegawax 250 column (Sigma) coupled with a flame ionization detector.
6
Evaluating Interference in COVID-19 Assays
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To evaluate interference by hemolytic, icteric, and lipemic serum conditions as well as high biotin and rheumatoid factor (RF) concentrations, assay mixtures were spiked with hemoglobin (Sigma), bilirubin (Sigma), triglycerides (Sigma), biotin (Sigma), and RF (Lee Biosolutions), respectively. Simulated serum concentrations were 5 mg/mL and 10 mg/mL for hemoglobin, 0.5 mg/mL for bilirubin, and 5 mg/mL for triglycerides. biotin interference was tested at simulated serum concentrations of between 0.1 ng/mL and 100,000 ng/mL; simulated RF serum concentrations were 50 IU/mL, 100 IU/mL, and 1,000 IU/mL. Assays were performed by employing three COVID-19 patient serum samples covering a range of A450-A620 readings (“low,” “medium,” and “high”) and at least three prepandemic negative-control serum samples.
7
Comprehensive Lipid and Catecholamine Analysis
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Paraformaldehyde, sucrose, Oil Red O (ORO), Sirius red, hematoxylin, pyridine, N,O-bis(trimethylsilyl)trifluoroacetamide (BSTFA), ammonium formate, ammonium acetate, dopamine, dopamine-d4, 3-methoxytyramine, 3-methoxytyramine-d4, norepinephrine, norepinephrine-d6, epinephrine, epinephrine-d6, normetanephrine, normetanephrine-d3, metanephrine, metanephrine-d3, nonadecanoic acid C19, triglycerides, and cholesterol standards were purchased from Sigma-Aldrich (St. Louis, MO, USA). Methanol, chloroform, hexane-acetonitrile, formic acid, and hydrochloric acid were acquired from Merck (Kenilworth, NJ, USA). Water was treated with a Purelab® ultra analytic purification system from ELGA LabWater (High Wycombe, UK). PBS was obtained from Gibco (New York, NY, USA).
8
Quantitative Liver Lipid Analysis
9
Milk Composition Analysis in Mice and Rats
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Milk samples from dams were collected by manual milking from anesthetized 15-day lactating mice and rats. FGF21 protein levels in milk and plasma were determined by ELISA (BioVendor). Milk analysis included measurement of triglycerides and lactose (both from Sigma-Aldrich), protein (Bio-Rad), individual fatty acid composition26 (link), adiponectin (Invitrogen), leptin (Millipore).
10
Plasma Lipid Profiling Procedure
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Blood samples were collected in EDTA-containing tubes, and centrifuged at 3000 rpm for 5 min to obtain plasma, which was stored at −20 °C for analysis. Plasma Total cholesterol and triglycerides were determined using enzymatic kits (Total cholesterol, Wako (LabAssay); triglycerides (Sigma)) according to the manufacturers’ instructions.
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