Silica gel 60 f254 coated plates

Solvents were procured from Merck. Thin layer chromatography (TLC), was performed on Silica gel 60 F₂₅₄ coated plates (Merck) to check the purity of compounds. For performing FT-IR, Perkin Elmer FT-IR spectrophotometer was used. Melting points were determined by Gallenkamp melting point apparatus. ¹H and ¹³C NMR were recorded in Bruker NMR 500/700 MHz and 125/176 MHz spectrophotometer. The samples were run in DMSO-d₆ with tetra methyl silane (TMS) as an internal standard. Molecular masses of compounds were determined in GC mass spectroscopy. The CHN Elementar (Analysensysteme GmbH, Germany) was used for elemental analysis of the compounds. Cisplatin was purchased from Sigma-Aldrich, USA. Antibodies against COX-2 and β-actin were purchased from Abcam (Cat Log No: ab15191).

The solvents were procured from Merck. Purity of the synthesized compounds was confirmed by thin layer chromatography (TLC), performed on Silica gel 60 F₂₅₄ coated plates (Merck, Kenilworth, NJ, USA). UV light was used for the visualization of TLC spots. Spectrum BX, Perkin Elmer FT-IR spectrophotometer (Perkin Elmer, Hopkinton, MA, USA) was used for performing FTIR. Gallenkamp melting point apparatus (Gallenkamp, Loughborough, UK) was used for performing melting points. Bruker NMR 500 MHz and 125 MHz spectrophotometer (Bruker, Billerica, MA, USA) were used for ¹H- and ¹³C-NMR. All the samples were processed in DMSO-d₆ with tetramethylsilane (TMS) as internal standard. Mass spectroscopy was used for the measurement of molecular masses of compounds. The elemental analysis of the compounds was performed on the CHN Elementar (Analysensysteme GmbH, Langenselbold, Germany). The elemental analyses were within the limit.

All the solvents were purchased from Merck (Kenilworth, NJ, USA). To check the purity of compounds, thin layer chromatography (TLC), was performed on silica gel 60 F₂₅₄ coated plates (Merck). For performing FTIR, Perkin Elmer (Waltham, MA, USA) FT-IR spectrophotometer was used. Melting points were measured by Gallenkamp melting point apparatus. ¹H and ¹³C NMR were recorded in Bruker (Billerica, MA, USA) NMR 500/700 MHz and 125/176 MHz spectrophotometer. The samples were run in DMSO-d₆ with tetramethyl silane (TMS) as an internal standard. Molecular weights of compounds were determined in mass spectroscopy. The elemental analysis of compounds was performed by CHN Elementar (Analysensysteme GmbH, Langenselbold, Germany). The compound (IIb) was obtained as single crystal by reported method. Data were collected on a Bruker APEX-II D8 Venture area diffractometer. SHELXT was used to solve structure [41 (link),42 ]. CCDC 1532829 contains the supplementary crystallographic data for the compound (IIb). These data can be obtained free of charge via http://www.ccdc.cam.ac.uk/conts/retrieving.html (or from the CCDC, 12 Union Road, Cambridge CB2 1EZ, UK; Fax: +44 1223 336033; E-mail: deposit@ccdc.cam.ac.uk).