β lactoglobulin β lg

Manufactured by Merck Group

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β-lactoglobulin (β-LG) is a protein found in the whey fraction of milk. It is a member of the lipocalin family of proteins and has the ability to bind and transport small hydrophobic molecules. β-LG is commonly used in research and biochemical applications as a model system for studying protein structure, function, and interactions.

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β-lactoglobulin (72 citations) β-lactoglobulin (β-LG) from bovinemilk (3 citations)

5 protocols using β lactoglobulin β lg

Immunoassay protocol for HPV detection

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3-Hydroxyphthalic anhydride (HP), 2,4,6-trinitrobenzenesulfonic acid (TNBS), human serum albumin (HSA), β-lactoglobulin (β-LG), chicken ovalbumin (OVA), and bovine serum albumin (BSA) were purchased from Sigma. Horseradish peroxidase (HRP)-conjugated goat anti-human IgA and IgE antibodies were purchased from Abcam (UK). HRP-conjugated rabbit anti-mouse IgG antibody was purchased from Dako (Denmark). The HPV16- and HPV-58-L1/L2-expressing plasmids and Luciferase pCLucf plasmid were kindly provided by Dr. John Schiller at the Laboratory of Cellular Oncology, National Cancer Institute, NIH, MD, USA.

Hua C., Zhu Y., Wu C., Si L., Wang Q., Sui L, & Jiang S. (2019). The Underlying Mechanism of 3-Hydroxyphthalic Anhydride-Modified Bovine Beta-Lactoglobulin to Block Human Papillomavirus Entry Into the Host Cell. Frontiers in Microbiology, 10, 2188.

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Purification and Analysis of β-Lactoglobulin

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β-Lactoglobulin (β-LG, ≥90% purity, lyophilized powder) was obtained from Sigma-Aldrich (St. Louis, MO, USA). The 3,3′-Diindolylmethane (>99% purity) compound was purchased from Luotian Pharmaceutical Co., Ltd. (Hubei, China). Potassium bromide was purchased from Sigma-Aldrich (St. Louis, MO, USA). All other chemicals were obtained from Beijing Chemical Works (Beijing, China).

Wang C., Zhou X., Wang H., Sun X, & Guo M. (2019). Interactions between β-Lactoglobulin and 3,3′-Diindolylmethane in Model System. Molecules, 24(11), 2151.

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Protein Characterization by GPC

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Analytical grade chemicals were obtained from Carlo Erba (Milano, Italy). Bromelain was obtained from Nutraceutica (Bologna, Italy) (2400 GDU g⁻¹) and from Enzyme Development Corporation (New York, NY, USA). Pancreatin from porcine pancreas (4 USP) was purchased from Sigma-Aldrich (St. Louis, MI, USA).
Synthetic peptides used for Gel Permeation Chromatography (GPC) calibration were bought from Sigma-Aldrich (St. Louis, MI, USA) (i.e., bovine serum albumin (BSA), β-Lactoglobulin (β-Lg), α-lactalbumin (α-La), aprotinin, bacitracin, tetrapeptide (Leu-Trp-Met-Arg), Asp-Glu, Tyr).

Cabizza R., Fancello F., Petretto G.L., Addis R., Pisanu S., Pagnozzi D., Piga A, & Urgeghe P.P. (2021). Exploring the DPP-IV Inhibitory, Antioxidant and Antibacterial Potential of Ovine “Scotta” Hydrolysates. Foods, 10(12), 3137.

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In-Vitro Cytotoxicity Assay Protocol

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Silver nitrate (≥ 98.0%), sodium sulfide nonahydrate (≥ 98.0%), sodium hydroxide (≥ 98.0%) and β-lactoglobulin (β-LG, MW: ~18.4 kDa) were purchased from Sigma-Aldrich. 3-(4,5-Dimethylthiazol-2yl)-2,5-diphenyltetrazolium bromide (MTT), DMEM, FBS, penicillin, streptomycin and trypsin-EDTA were purchased from commercial sources. Ultrapure Millipore water (resistivity: 18.2 mΩ•cm) was used make solutions and buffers. All other reagents were of at least analytical grade and used without further purification.

Chen J ., Kong Y ., Wo Y ., Fang H ., Li Y ., Zhang T ., Dong Y ., Ge Y ., Wu Z ., Zhou D , & Chen S . (2016). Facile synthesis of β-lactoglobulin capped Ag₂S quantum dots for in vivo imaging in the second near-infrared biological window. Journal of materials chemistry. B, 4(37).

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Analytical Characterization of Milk Proteins

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Pure α-lactalbumin (α-La) (code L6010) and β-lactoglobulin (β-Lg) (code L0130) and single amino acids were purchased from Sigma-Aldrich (Milan, Italy). Nynhydrin was purchased from Biochrom Ltd (Cambridge, UK). All chemicals were of analytical grade.

Pellegrino L., Rosi V., D'Incecco P., Stroppa A, & Hogenboom J.A. (2015). Changes in the soluble nitrogen fraction of milk throughout PDO Grana Padano cheese-making. International Dairy Journal., 47.

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