Anti alb

Frozen sections(7 µm‐thick) were fixed with 4% PFA for 30 min, and then washed by PBS for three times. The samples were permeabilized and blocked by PBS containing 0.3% Triton X‐100 (Sigma) and 3% bovine serum albumin (BSA) (Amresco) for 1 h. The primary antibody was incubated overnight at 4 °C. The following primary antibodies were used: anti‐COL‐1 (Abcam, ab6308), anti‐αSMA (eBioscience, 14‐9760‐82), anti‐F4/80 (Abcam, ab6640), anti‐CD80 (Biolegend, 104 705), anti‐Arg1 (Cell Signaling Technology, 93 668), anti‐CD31 (Abcam, ab9498), anti‐Ki67 (eBioscience, 12‐5698‐82), and anti‐ALB (Abcam, ab207327). The samples were incubated with fluorescent secondary antibody for 1 h at room temperature. The samples were washed by PBS for three times. Collagen was stained by Sirius Red staining (Huayueyang Bio‐Technology, GH6044s) according to manufacturer's instructions. In situ zymography was performed by using in situ zymography kit (Genmed, GMS80095.1) according to manufacturer's instructions. Histological images were taken using a microscope (3DHISTECH Pannoramic SCAN) or a confocal laser scanning microscope (Olympus FV3000). Image analysis was carried out using Imaris 9.6.0 with a consistent setup.

The following antibodies were used: anti-ALB (Abcam, ab8940, 1:400); anti-β-actin (Santa Cruz Biotechnology, sc-130301, 1:5 000); anti-caldesmon (Sigma-Aldrich, C4562, 1:300); anti-CD73 (BD Biosciences, 550741, 1:50); anti-CD90 (BD Biosciences, 555595, 1:100); anti-CD105 (eBioscience, 1-1057, 1:100); anti-CENPA (Abcam, ab13939, 1:400); anti-fibrillarin (Abcam, ab4566, 1:100); anti-FLAG (Sigma-Aldrich, M2, 1:2 000 for western blotting, 1:400 for immunofluorescence); anti-GAL4 (Abcam, ab14477, 1:1 000 for western blotting, 1:400 for immunofluorescence); anti-γH2AX (Millipore, 05-636, 1:400); anti-IgG-APC (eBioscience, 555751, 1:100); anti-IgG-FITC (eBioscience, 555748, 1:100); anti-IgG-PE (eBioscience, 555749, 1:100); anti-MAP2 (Sigma-Aldrich, m4403, 1:500); anti-NANOG (Abcam, ab21624, 1:250); anti-nestin (Millipore, MAB5326, 1:500); anti-NeuN (Millipore, ABN78 1:400); anti-OCT4 (Santa Cruz Biotechnology, sc-5279, 1:100); anti-Nucleolin (Abcam, ab22758, 1:200); anti-PAX6 (Covance, PRB-278P, 1:500); anti-SMA (Sigma-Aldrich, A5228, 1:100); anti-SM22 (Abcam, ab14106, 1:200); anti-SOX2 (Santa Cruz Biotechnology, sc-17320, 1:100); anti-Tuj1 (Sigma-Aldrich, T2200, 1:500); Alexa Fluor 555-conjugated wheat germ agglutinin (Thermo Fisher, W32464, 1:500). Other reagents, including deoxycytidine, hydroxyurea, NAC, nocodazole, and thymidine, were purchased from Sigma-Aldrich.