Plasma samples for pharmacokinetic characterization were collected at 5, 15, and 30 minutes as well as at 1, 2, 4, and 6 hours following i.v. bolus injection of TH1902 into normal CD‐1 mice with lithium heparin microvette tubes (Sarstedt). TH1902 and docetaxel concentrations were determined by UPLC/MS. Briefly, both TH1902 and docetaxel were extracted from 50 µL of plasma using 4 volumes of cold acetonitrile (87%):acetic acid (0.125%):water (12.875%) in micro centrifuge tubes. After 5 minutes on ice, the suspensions were centrifuged at 10 000 g for 5 minutes and supernatants (2‐10 µL) were injected into a Waters Acquity UPLC spectrometer coupled to a microTOF spectrometer from Bruker using electron spray ionization (ESI‐TOF). TH1902 and released docetaxel from the peptide were quantified by peak area ratio at a wavelength of 220 nm. Standard curves for TH1902 and docetaxel were performed in mouse plasma. A linear regression was carried out to determine the concentration of TH1902 and docetaxel. The limit of quantitation for TH1902 and for free docetaxel was around 8 µM.
Lithium heparin microvette tubes
Manufactured by Sarstedt
Lithium heparin microvette tubes are blood collection devices designed for the collection and storage of blood samples. They contain lithium heparin as an anticoagulant to prevent blood clotting. The tubes are compact and convenient for use in laboratory settings.
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Lab products found in correlation
2 protocols using lithium heparin microvette tubes
1
Pharmacokinetic Characterization of TH1902
2
Glucose Homeostasis Assessment Protocols
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Fed blood glucose was monitored regularly from small tail incisions after administration of a local anaesthetic (EMLA cream, Eutectic Mixture of Local Anesthetics, Lidocaine/Prilocaine, AstraZeneca), using Lithium-Heparin microvette tubes (Sarstedt). Plasma glucose was measured using an Analox Glucose Analyser GM9.
IPGTTs were conducted after an overnight fast. Blood glucose was measured by tail incision immediately before, and then 30, 60 and 120 minutes after injection with 2 g of glucose per kg body weight (20% glucose in 0.9% NaCl). An additional time point (15 minutes) was added for the IPGTT at 13 weeks of age.
IPITTs were conducted after a 4h fast. Blood glucose was measured by tail incision immediately before and 15, 30, 45, 60 and 90 minutes after intraperitoneal injection of insulin (0.75 U/kg
BW).
IPGTTs were conducted after an overnight fast. Blood glucose was measured by tail incision immediately before, and then 30, 60 and 120 minutes after injection with 2 g of glucose per kg body weight (20% glucose in 0.9% NaCl). An additional time point (15 minutes) was added for the IPGTT at 13 weeks of age.
IPITTs were conducted after a 4h fast. Blood glucose was measured by tail incision immediately before and 15, 30, 45, 60 and 90 minutes after intraperitoneal injection of insulin (0.75 U/kg
BW).
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