Waters 1525 binary hplc system

Manufactured by Waters Corporation

Sourced in United States

The Waters 1525 binary HPLC system is a high-performance liquid chromatography instrument designed for analytical and preparative separation and purification of chemical compounds. It features a binary solvent delivery system, an integrated column compartment, and a variety of detection options to meet the needs of diverse applications.

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Lab products found in correlation

Waters 2487 dual λ absorbance detector, by Waters Corporation (2 mentions) Waters 2478 dual λ absorbance detector, by Waters Corporation (1 mentions) Eurospher 100 5 c18, by Knauer (1 mentions) Eurospher 100 5 c18 column, by Knauer (1 mentions)

4 protocols using waters 1525 binary hplc system

HPLC Analysis of Triterpenes and Sterols

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In this study, the identification and quantitative determination of inotodiol, cholesterol, and lanosterol were performed using a Waters 1525 Binary HPLC system (Waters, Milford, MA, USA) equipped with an evaporative light-scattering detector (Alltech 2000, Deerfield, IL, USA) and a YMC-triart C18 column (4.6 mm × 250 mm, YMCKOREA, Seongnam, Republic of Korea). The mobile phase gradients and instrument conditions were presented in Table S1 of Supplementary Data S1. All samples were run in replicates, with each being repeated at least three times.

Nguyen P.C., Nguyen M.T., Truong B.T., Kim D.R., Shin S., Kim J.E., Park K.B., Park J.H., Tran P.L., Ban S.Y., Kim J, & Park J.T. (2023). Isolation, Physicochemical Characterization, and Biological Properties of Inotodiol, the Potent Pharmaceutical Oxysterol from Chaga Mushroom. Antioxidants, 12(2), 447.

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Ginsenoside Profiling and Oral Administration

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UGBE and GBE water solvent extracts were kindly provided by Professor Sung Kwon Ko of Semyung University. We analyzed the constituents of UGBE and GBE bythe Waters 1525 binary HPLC system (Waters, Milford, MA, USA). Separation of UGBE was performed on an analytical column (Eurospher, 100-5 C18, 250 mm × 3.0 mm, 5μm; Knauer, Berlin, Germany) by gradient elution at room temperature. The eluent was a mixture of acetonitrile for HPLC (A) and distilled water for HPLC (B). The elution process was performed according to the following conditions: 0 min, 17% of A; 25 min, 25% of A; 50 min, 40% of A; 105 min, 60% of A; 110 min, 100% of A. The flow rate was 0.8 mL/min, injection volume was 20μL, and chromatograms were acquired by a UV/VIS Waters 2478 Dual λ Absorbance Detector (Waters) at 203 nm. After 10 h of ultrasonification, ginsenosides Rb1, Rb2, Rd, Re, Rf, Rg1, Rg2, Rg3, Rg6, Rh1, Rh4, Rk1, Rk3, F1, and F4 were identified. UGBE solution was orally administered to the rats once/d at doses of 100 mg/kg body weight (b.w.), 250 mg/kg b.w., and 500mg/kg b.w.

Nam Y., Bae J., Jeong J.H., Ko S.K, & Sohn U.D. (2017). Protective effect of ultrasonication-processed ginseng berry extract on the D-galactosamine/lipopolysaccharide-induced liver injury model in rats. Journal of Ginseng Research, 42(4), 540-548.

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Helleramine Isolation via Cation Exchange

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Isolation of helleramine was achieved by one chromatographic step. Venom sample (30 mg) was dissolved in 1 mL of 20 mM Tris-HCl pH 8.0 buffer and filtered through a 0.45 µm filter. Two hundred microliters of clear supernatant were injected into a cationic exchange column (Sulfopropyl Waters Protein Pak 7.5 × 75 mm-10 µm, Waters, MA, USA) equilibrated with 20 mM Tris-HCl, pH 8.0 buffer at a 1 mL/min flow rate. The eluting buffer was integrated linearly from 0 to 100% using a 20 mM Tris-HCl, pH 8.0 buffer containing 0.5 M NaCl. The proteins were eluted at a 1 mL/min flow rate over 90 min using a Waters™ 1525 binary HPLC system (Waters, MA, USA), and the elution profile was monitored at 280 nm using a Waters™ 2487 Dual λ absorbance detector (Waters, MI, USA). The active fractions were pooled, lyophilized, and stored at −20°C until used.

Salazar E., Rodriguez-Acosta A., Lucena S., Gonzalez R., McLarty M.C., Sanchez O., Suntravat M., Garcia E., Finol H.J., Giron M.E., Fernandez I., Deba F., Bessac B.F, & Sánchez E.E. (2020). Biological activities of a new crotamine-like peptide from Crotalus oreganus helleri on C2C12 and CHO cell lines, and ultrastructural changes on motor endplate and striated muscle. Toxicon : official journal of the International Society on Toxinology, 188, 95-107.

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HPLC Analysis of Ginsenoside Composition

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The ginsenoside composition of the concentrate was analyzed by HPLC, as suggested by Ko and colleagues [13,21] . The total ginsenoside content and composition of each sample were analyzed three times. The 99% pure ginsenoside standards used in this experiment were purchased from Chromadex and the Ambo Institute. For the experiment, the Waters 1525 binary HPLC system (Waters, Milford, MA, USA) and the Eurospher 100-5 C 18 column (3 × 250 mm; Knauer, Berlin, Germany) were used. The mobile phase was a mixture of acetonitrile (HPLC grade) and distilled water (HPLC grade). The content of acetonitrile was sequentially increased from 17% to 30% (35 min), from 30% to 40% (60 min), from 40% to 60% (100 min), from 60% to 80% (110 min), from 80% to 80% (120 min), from 80% to 100% (125 min), from 100% to 100% (135 min), and finally from 100% back to 17% (140 min, lasting for 5 min). The operating temperature was at room temperature and the flow rate was 0.8 mL/min. The elution profile on the chromatogram was obtained by using a UV/VIS detector at 203 nm (Waters 2487 dual λ absorbance detector; Waters) (Fig. 1A).

Kim S.J, & Kim A.K. (2014). Anti-breast cancer activity of Fine Black ginseng (Panax ginseng Meyer) and ginsenoside Rg5. Journal of Ginseng Research, 39(2), 125-134.

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